Preparation, characterization and pharmacological evaluation of topical hyalurosomes nanogel formulation of Calotropis gigantea for the treatment of Vitiligo
Keywords:
hyalurosomes, calotropis gigantea, HPLC, B16F10 cell line, cell uptakeAbstract
Objective: The objective of the paper is to preparation, characterization and pharmacological evaluation of topical hyalurosomes nanogel formulation of Calotropis Gigantea for the treatment of Vitiligo. Material and methods: Fresh milky white latex of Calotropis Gigantea was collected by cutting the stem twigs and identified by the HPLC method for phytoconstituents. Hyalurosomes as nanogel formulation was prepared by the solvent evaporation-based ethanol injection method. The nanogel was characterized for the particle size, Zeta-potential, poly dispersity index, transmission electron microscope, viscosity, entrapment efficiency and In-vitro release. The Carbopol based gel formulation was prepared and compare with the nanogel formulation. Fluorescent Rhodamine loaded hyalurosomes nanogel formulation (CGHGF) was prepared and utilized for cell uptake studies. The nanogel for anti-vitiligo activity was evaluated by cell uptake studies, cell viability assay, melanin content assay, tyrosinase activity assay and Fontana-Masson silver staining on B16F10 cell line. Result: The compounds present in the latex of Calotropis Gigantea includes lupeol, beta-amyrin, alpha-calotropeol, and beta-calotropeol, and tetracyclic compounds, including uscharin and 20-epi-uscharin, were characterized using HPLC method. The prepared hyalurosomes nanogel having the particle size 154.90±1.7, Poly-dispersity index was found to be 0.28 with zeta potential -17.00.
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