Characterization of clinical and community acquired staphylococcus aureus, local isolates

Ahmed J. Obaid; Melad K. Mohammed; Ahmed D. Jabbar

doi:10.53730/ijhs.v6nS7.13687

Authors

Ahmed J. Obaid Ministry of Health , Health Department, Wasit, Kut, Iraq
Melad K. Mohammed Department of Biology, College of Sciences, University of Wasit, Kut, Iraq
Ahmed D. Jabbar Department of Biology, College of Sciences, University of of Wasit, Kut, Iraq

Keywords:

Staphylococcus aureus, antibiotic resistance, virulence genes, pathogenicity

Abstract

It was found that 46.6% of S. aureus were collected from nose swaps followed by ear (41.6%) and catheter tubes (11.8%). Results showed the highest resistance 100% for both classes of Aminopenicillins (Amoxicillin), cephalosporins 3Gr (Cefotaxime, Ceftriaxone, and Cefexime, and Carboxypencillin, as well as one group as 93.5% for class the β-lactam combination agents (Amoxicillin/ clavulanic acid). Many virulence factors and antibiotics resistance genes also being considered in this study, including erm, luk, hlg, fbl, Sea, MecA, seb, vat and tet. The highest frequency was in MecA genes 53 (88%) followed by sea genes 20 (33%), hlg and seb genes 18 (30%) for each one, erm genes 14 (23.3%) and luk genes 2 (3.3%), genes fbI, vat and tet were not detected in any isolate. Results shows no correlation concerning age, gender or the site of infection with either antimicrobial resistance or virulence factors genes.

Downloads

Download data is not yet available.

References

Achek, R.; El-Adawy, H.;Hotzel, H.; Hendam, A.; Tomaso, H.;Ehricht, R.; Neubauer, H.; Nabi, I.;Hamdi, T.M. and Monecke, S.(2021). MolecularCharacterization of Staphylococcusaureus Isolated from Human andFood Samples in Northern Algeria.Pathogens . 10, 1276. https:// doi.org/10.3390/pathogens10101276.

Al-Talib ,H.; Yean,C.Y.; Al-Khateeb,A.; Hassan,H.; Banga Singh,K. ; Al-Jashamy,K. and Ravichandran.M.(2009). A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin.BMC Microbiol. 2009 May 28;9:113. doi: 10.1186/1471-2180-9-113.

Belthur, M.V.; Birchansky, S.B.; Verdugo, A.A.; Mason, E.O.; Hulten, K.G.; Kaplan, S.L.; Smith, E.O.; Phillips, W.A. and Weinberg, J.(2012). Pathologic fractures in children with acute Staphylococcus aureus osteomyelitis. J Bone Joint Surg Am 2012;94:34-42.

Diep, BA., Otto, M.( 2008). The role of virulence determinants in community-associated MRSA pathogenesis, Trends in Microbiol. 16: 361-369.

Gordon, Y. C.; Cheung, J. S. Bae and Michael O. (2021). Pathogenicity and virulence of Staphylococcus aureus, Virulence, 12:1, 547-569, DOI: 10.1080/21505594.2021.1878688.

https://doi.org/10.1111/j.13652672.2011.05017.x.

Lowy FD (2003) Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Invest 111(9):1265–1273.

Makinde, T. M., Ako-Nai, K. A., & Shittu, A. O. (2019). A study on the

antibiotic susceptibility of Staphylococcus aureus from nasal samples of

female students at the Obafemi Awolowo university campus. J Microbiol

Exp, 7(2), 78–83.

Montanaro, L.; Testoni, F.; Poggi, A.; Visai, L.; Speziale, P.and Arciola, C.R.(2011). Emerging pathogenetic mechanisms of the implant-related osteomyelitis by Staphylococcus aureus. Int J Artif Organs 2011;34:781-8.

O’Hara, F., Guex, N., Word, J., Miller, L., Becker, J., Walsh, S., Scangarella, N., West, J., Shawar, R., Amrine-Madsen, H. (2008). A Geographic Variant of the Staphyloccus aureus PantonValentine Leukocidin Toxin and the Origin of CommunityAssociated Methicillin-Resistant S. aureus USA300, J. Infect. Dis. 197: 187-94.

Roberts, M.C.; Soge, O.O.; No, D.;Helgeson, S.E. and Meschke, J.S.(2011). Characterization of Methicillin-resistant Staphylococcus aureus isolated from public surfaces on a University Campus, Student Homes and Local Community.

Roemer, T.; Schneider, T.and Pinho, M.G.(2013). Auxiliary factors: a chink in the armor of MRSA resistance to beta-lactam antibiotics. Curr Opin Microbiol 2013;16:538-48.

Shariati, A., Dadashi, M., Moghadam, M. T., van Belkum, A., Yaslianifard, S., & Darban-Sarokhalil, D. (2020). Global prevalence and distribution of vancomycin resistant, vancomycin intermediate and heterogeneously vancomycin intermediate Staphylococcus aureus clinical isolates: a systematic review and meta-analysis. Scientific reports, 10(1), 1-16.

Strommenger ,B.; Kettlitz,C.; Werner,G. and Wolfgang Witte,W.(2003). Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J Clin Microbiol. Sep;41(9):4089-94. doi: 10.1128/JCM.41.9.4089-4094.

Voyich, JM., Otto, M., Mathema, B., Braughton, KR., Whitney, AR., Welty, D., Long, RD., Dorward, DW., Gardner, DJ., Lina, G., Kreiswirth, BN., DeLeo, FR. (2006). Is Panton-Valentine Leukocidin the Major Virulence Determinant in Community-Associated Methicillin-Resistant Staphylococcus aureus Disease, J. Infect. Dis. 194: 1761-1770.