RPA-Based colorimetric detection of SARS-Cov-2 (Covid-19) and its physiological effects
Keywords:
Recombinase Polymerase Amplification, COVID-19, real time quantitative polymerase chain reaction, physiological problemsAbstract
The SARS-CoV-2 coronavirus outbreak is extremely concerning and poses a threat to the public health system. SARS-CoV-2 is a pathogen that affects people and caused fever, dry cough, dizziness and severe respiratory disease. By media time, greater than 662 million people were infected and 6.6 million people were died globally. The pandemic coronavirus was hurt the third world countries due to weak health infrastructure. A timely response is crucial for commercially and easily accessible resources since an actual pandemic emergency does not provide the necessary timescale for the test of innovative ways. Coronavirus SARS-CoV-2 was infected individuals without any indication and could still transfer the virus to others. The success of the quarantine effort during the SARS-CoV-2 eruption depended heavily on the identification of the infectious agent. It is thus urgent to develop a rapid and accurate detection method for coronavirus SARS-CoV-2 and control the disease spreading. Here demonstrated an isothermal based SARS-CoV-2 amplification with quick colorimetric detection. The inorganic phosphate (Pi) was detected via colorimetric technique from SARS-CoV-2 after amplification by using the basic recombinase polymerase amplification (RPA) technique.
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