Anti-diabetic potential of water-soluble polysaccharide from okra pods mucilage diabetes
Keywords:
Okra, abelmoschus esculentus, Polysaccharides, antidiabeticAbstract
Plant derived mucilage, due its role in promoting human health, has been extensively used as active constituent for the preparation of different pharmaceuticals, functional and nutraceutical products. Mucilage mainly consists of complex carbohydrates with extremely branched structure. Abelmoschus esculentus L. (Moench), belongs to family Malvaceae, is an annual herb commonly known as okra. The purpose of this study was the identification of bioactive polysaccharides present in the mucilage of okra pods and evaluation of their biological activities. Mucilage was extracted by soaking okra pods in water. The dried mucilage was fractionated in 75% ethanol, which resulted in soluble and insoluble fractions. Each fraction was screened which confirmed the presence of galacturonic acid, saturated and unsaturated polysaccharides. Crude, soluble and insoluble fractions were processed for investigating their biological activities. The results indicated that insoluble fraction was able to inhibit the growth of five human pathogenic bacterial strains i.e. Staphylococcus aureus (1.9 mm ± 0.27mm), Escherichia coli (2.8mm ± 0.44mm) and Shigella (2.9mm ± 0.85mm). However, no significant inhibition was observed against Klebsiella pneumonia and Salmonella typhi. Antioxidant capacity was determined through DPPH assay where the soluble fraction showed IC50 value 785.5 μg/mL while the insoluble fraction showed IC50 value 987.21 μg/mL.
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References
Agregán, R., M. Pateiro, et al. (2022). "Biological activity and development of functional foods fortified with okra (Abelmoschus esculentus)." Critical Reviews in Food Science and Nutrition: 1-16.
Aloysius, M., K. N. Felekkis, et al. (2022). "Chitosan Nanogel with Mixed Food Plants and Its Relation to Blood Glucose in Type 2 Diabetes: A Systematic and Meta-Analysis Review of Observational Studies." Nutrients 14(22): 4710.
Ameena, K., C. Dilip, et al. (2010). "Isolation of the mucilages from Hibiscus rosasinensis linn. and Okra (Abelmoschus esculentus linn.) and studies of the binding effects of the mucilages." 3(7): 539-543.
Dantas, T. L., F. C. Alonso Buriti, et al. (2021). "Okra (Abelmoschus esculentus L.) as a potential functional food source of mucilage and bioactive compounds with technological applications and health benefits." Plants 10(8): 1683.
Farooq, U., R. Malviya, et al. (2013). "Extraction and characterization of okra mucilage as pharmaceutical excipient." 6(4): 168-172.
Gemede, H. F., N. Ratta, et al. (2015). "Nutritional quality and health benefits of okra (Abelmoschus esculentus): A review." 6(458): 2.
Hakeem, R. and A. J. J. o. d. Fawwad (2010). "Diabetes in Pakistan: epidemiology, determinants and prevention." 1(3): 3.
Haque, M. A., M. S. Hossain, et al. (2022). "Abelmoschus esculentus (L.) Moench Pod Extract Revealed Antagonistic Effect against the Synergistic Antidiabetic Activity of Metformin and Acarbose upon Concomitant Administration in Glucose-Induced Hyperglycemic Mice." 2(2): 128-138.
Kaul, K., J. Tarr, et al. Introduction to diabetes mellitus. InDiabetes 2013 (pp. 1-11), Springer, New York, NY.
Liu, Y., J. Qi, et al. (2021). "Okra in food field: Nutritional value, health benefits and effects of processing methods on quality." Food Reviews International 37(1): 67-90.
Pasha, A. Z., S. A. Bukhari, et al. (2022). "Evaluation of Modified Date Palm (Phoenix dactylifera L.) Mucilage as a Potential Pharmaceutical Excipient." 2022.
Raj, V., J.-J. Shim, et al. (2020). "Grafting modification of okra mucilage: Recent findings, applications, and future directions." Carbohydrate polymers 246: 116653.
Selvarasu, K. and A. Maiyappan "DIABETES AND CHRONIC DISEASES: A CORRELATIONAL STUDY."
Tosif, M. M., A. Najda, et al. (2021). "A comprehensive review on plant-derived mucilage: Characterization, functional properties, applications, and its utilization for nanocarrier fabrication." Polymers 13(7): 1066.
Zhao, Z., X. Dai, et al. (2020). "Pyrazolone structural motif in medicinal chemistry: Retrospect and prospect." European Journal of Medicinal Chemistry 186: 111893.
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