A study on the serum arginase and nitrate levels in association with Fev1/Fvc ratio in chronic obstructive pulmonary disease

D S S K Raju; Anil Kumar Munta; D Lakshmi Lalitha

doi:10.53730/ijhs.v6nS9.14067

Authors

D S S K Raju
dsskraju@gmail.com
Assistant Professor, Department of Biochemistry, Maharajah’s Institute of Medical Sciences Nellimarla, Vizianagaram Andhra Pradesh – India – 535217
Anil Kumar Munta Professor, Department of Biochemistry, Madhubani Medical College, Madhubani, Bihar (India) – 847212
D Lakshmi Lalitha Principal & Professor of Biochemistry, Great Eastern Medical School Ragolu, Srikakulam Andhra Pradesh, India - 532484

Keywords:

Chronic Obstructive Pulmonary Disease, FEV1/FVC ratio, Arginase, nitrate and Neutrophil Lymphocyte Ratio

Abstract

COPD is generally associated with chronic inflammation in peripheral airway and lung parenchyma which finally leads to obstruction of the airways. The diagnostic criteria for COPD is FEV1/FVC ratio which is less than 0.7. Arginase being the key enzyme of urea cycle, is also expressed in extra hepatic tissue including lung in the bronchial epithelial cells and endothelial cells. The main function of arginase here, is to regulate the formation of nitric oxide, indirectly competing for the common substrate L- arginine. The NO forms peroxynitrite on reaction with reactive oxygen species due to oxidative stress. Peoxynitrite is unstable molecule and breaks down to nitrite and nitrate. The present research work was approved by Institutional Ethical committee. Informed consent was obtained from the patients and controls prior to the study. It is a case control study and it included 45 COPD patients between the age group of 40-60 years who attended the outpatient department of pulmonary medicine, Maharajah’s Institute of Medical Sciences, Nellimarla, Vizianagaram. These are labeled cases. 45 normal healthy age and sex matched individuals with normal FEV1/FVC ratio are considered as controls.

Downloads

Download data is not yet available.

References

Vogelmeier CF, Criner GJ, Martinez FJ, Anzueto A, Barnes PJ, Bourbeau J, Celli BR, Chen R, Decramer M, Fabbri LM, Frith P. Global Strategy for the Diagnosis, Management and Prevention of Chronic Obstructive Lung Disease 2017 Report. Respirology. 2017 Apr 1; 22(3):575-601.

Soriano JB, Rodríguez-Roisin R. Chronic obstructive pulmonary disease overview: epidemiology, risk factors, and clinical presentation. Proceedings of the American Thoracic Society. 2011 Aug 1; 8(4):363-7.

Agusti A, Soriano JB, COPD as a Systemic Disease. Journal of Chronic Obstructive Pulmonary Disease. 2008: 5, 133-138.

Munder M, Mollinedo F, Calafat J, Canchado J, Gil-Lamaignere C, Fuentes JM, Luckner C, Doschko G, Soler G, Eichmann K, Müller FM. Arginase I is constitutively expressed in human granulocytes and participates in fungicidal activity. Blood. 2005 Mar 15; 105(6):2549-56.

Bergeron C, Boulet LP, Page N, Laviolette M, Zimmermann N, Rothenberg ME, Hamid Q. Influence of cigarette smoke on the arginine pathway in asthmatic airways: increased expression of arginase I. Journal of Allergy and Clinical Immunology. 2007 Feb 28; 119(2):391-7.

Xia Y, Roman LJ, Masters BS, Zweier JL. Inducible nitric-oxide synthase generates superoxide from the reductase domain. Journal of Biological Chemistry. 1998 Aug 28; 273(35):22635-9.

Maarsingh H, Pera T, Meurs H. Arginase and pulmonary diseases. Naunyn- Schmiedeberg's archives of pharmacology. 2008 Aug 1; 378(2):171.

British Thoracic Society. Guidelines for the management of COPD. Thorax 1997; 52: 5, 1–28.

Moshage H, Kok B, Huizenga JR, Jansen PL. Nitrite and nitrate determinations in plasma: a critical evaluation. Clinical chemistry. 1995 Jun 1; 41(6):892-6.

American Thoracic Society. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 1995; 152:77S-120S.

Ahsen A, Ulu MS, Yuksel S, Demir K, Uysal M, Erdogan M, Acarturk G. As a new inflammatory marker for familial Mediterranean fever: neutrophil-to-lymphocyte ratio. Inflammation. 2013 Dec 1; 36(6):1357-62.

Aaron SD, Angel JB, Lunau M, Wright K, Fex C, Le Saux N, Dales RE. Granulocyte inflammatory markers and airway infection during acute exacerbation of chronic obstructive pulmonary disease. American journal of respiratory and critical care medicine. 2001 Feb 1; 163(2):349-55.

Torun S, Tunc BD, Suvak B, Yildiz H, Tas A, Sayilir A, Ozderin YO, Beyazit Y, Kayacetin E. Assessment of neutrophil-lymphocyte ratio in ulcerative colitis: a promising marker in predicting disease severity. Clinics and research in hepatology and gastroenterology. 2012 Oct 1; 36(5):491-7.

Azab B, Jaglall N, Atallah JP, Lamet A, Raja-Surya V, Farah B, Lesser M, Widmann WD. Neutrophil-lymphocyte ratio as a predictor of adverse outcomes of acute pancreatitis. Pancreatology. 2011; 11(4):445-52.

Cockayne DA, Cheng DT, Waschki B, Sridhar S, Ravindran P, Hilton H, Kourteva G, Bitter H, Pillai SG, Visvanathan S, Müller KC. Systemic biomarkers of neutrophilic inflammation, tissue injury and repair in COPD patients with differing levels of disease severity. PloS one. 2012 Jun 12; 7(6):e38629.

Barnes PJ. The cytokine network in chronic obstructive pulmonary disease. American journal of respiratory cell and molecular biology. 2009 Dec; 41(6):631-8.

Weathington NM, van Houwelingen AH, Noerager BD, Jackson PL, Kraneveld AD, Galin FS, Folkerts G, Nijkamp FP, Blalock JE. A novel peptide CXCR ligand derived from extracellular matrix degradation during airway inflammation. Nature medicine. 2006 Mar; 12(3):317.

Hoenderdos K, Condliffe A. The neutrophil in chronic obstructive pulmonary disease. Too little, too late or too much, too soon?. American journal of respiratory cell and molecular biology. 2013 May; 48(5):531-9.

Hodge S, Hodge G, Scicchitano R, Reynolds PN, Holmes M. Alveolar macrophages from subjects with chronic obstructive pulmonary disease are deficient in their ability to phagocytose apoptotic airway epithelial cells. Immunology and cell biology. 2003 Aug; 81(4):289.

Borges VM, Vandivier RW, McPhillips KA, Kench JA, Morimoto K, Groshong SD, Richens TR, Graham BB, Muldrow AM, Heule LV, Henson PM. TNFα inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation. American Journal of Physiology-Lung Cellular and Molecular Physiology. 2009 Oct; 297(4):L586-95.

Oudijk ED, Nijhuis EH, Zwank MD, Van de Graaf EA, Mager HJ, Coffer PJ, Lammers JJ, Koenderman L. Systemic inflammation in COPD visualised by gene profiling in peripheral blood neutrophils. Thorax. 2005 Jul 1; 60(7):538-44.

Juss JK, Hayhoe RP, Owen CE, Bruce I, Walmsley SR, Cowburn AS, Kulkarni S, Boyle KB, Stephens L, Hawkins PT, Chilvers ER. Functional redundancy of class I phosphoinositide 3-kinase (PI3K) isoforms in signaling growth factor-mediated human neutrophil survival. PLoS One. 2012 Sep 24; 7(9):e45933.

Munder M, Mollinedo F, Calafat J, Canchado J, Gil-Lamaignere C, Fuentes JM, Luckner C, Doschko G, Soler G, Eichmann K, Müller FM. Arginase I is constitutively expressed in human granulocytes and participates in fungicidal activity. Blood. 2005 Mar 15; 105(6):2549-56.

Renkema TE, Postma DS, Noordhoek JA, Sluiter HJ, Kauffman HF. In vitro release of neutrophil elastase, myeloperoxidase and beta-glucuronidase in patients with emphysema and healthy subjects. European Respiratory Journal. 1991 Nov 1; 4(10):1237-44.

Kharitonov SA, Barnes PJ. Exhaled markers of pulmonary disease. Am J Respir Crit Care Med. 2001; 163:1693–1722

Van den Berg MP, Meurs H, Gosens R. Targeting arginase and nitric oxide metabolism in chronic airway diseases and their co-morbidities. Current opinion in pharmacology. 2018 Jun 30; 40:126-33.

Ricciardolo FL, Sterk PJ, Gaston B, Folkerts G. Nitric oxide in health and disease of the respiratory system. Physiological reviews. 2004 Jul; 84(3):731-65.

Rengasamy AP, Johns RA. Regulation of nitric oxide synthase by nitric oxide. Molecular Pharmacology. 1993 Jul 1; 44(1):124-8.

Rahman I. Antioxidant therapies in COPD. International journal of chronic obstructive pulmonary disease. 2006 Mar; 1(1):15.

Persson MG, Gustafsson LE, Zetterström O, Agrenius V, Ihre E. Single-breath nitric oxide measurements in asthmatic patients and smokers. The Lancet. 1994 Jan 15; 343(8890):146-7.

Jain BH, Rubinstein I, Robbins RA, Leise KL, Sisson JH. Modulation of airway epithelial cell ciliary beat frequency by nitric oxide. Biochemical and biophysical research communications. 1993 Feb 26; 191(1):83-8.

Moncada S, Higgs A. The L-arginine-nitric oxide pathway. New England Journal of Medicine. 1993 Dec 30; 329(27):2002-12.

Saleh D, Ernst P, Lim S, Barnes PJ, Giaid A. Increased formation of the potent oxidant peroxynitrite in the airways of asthmatic patients is associated with induction of nitric oxide synthase: effect of inhaled glucocorticoid. The FASEB journal. 1998 Aug;12(11):929-37.

Hamid Q, Springall DR, Polak JM, Riveros-Moreno V, Chanez P, Bousquet J, Godard P, Holgate S, Howarth P, Redington A. Induction of nitric oxide synthase in asthma. The Lancet. 1993 Dec 18; 342(8886-8887):1510-3.

Okamoto T, Akaike T, Nagano T, Miyajima S, Suga M, Ando M, Ichimori K, Maeda H. Activation of human neutrophil procollagenase by nitrogen dioxide and peroxynitrite: a novel mechanism for procollagenase activation involving nitric oxide. Archives of biochemistry and biophysics. 1997 Jun 15; 342(2):261-74.

Ischiropoulos H, Zhu L, Chen J, Tsai M, Martin JC, Smith CD, Beckman JS. Peroxynitrite-mediated tyrosine nitration catalyzed by superoxide dismutase. Archives of biochemistry and biophysics. 1992 Nov 1; 298(2):431-7.

Beckman JS, Ischiropoulos H, Zhu L, van der Woerd M, Smith C, Chen J, Harrison J, Martin JC, Tsai M. Kinetics of superoxide dismutase-and iron-catalyzed nitration of phenolics by peroxynitrite. Archives of Biochemistry and Biophysics. 1992 Nov 1; 298(2):438-45.

Van der Vliet A, Eiserich J. P., Shigenaga M. K., and. Cross C. E. Reactive nitrogen species and tyrosine nitration in the respiratory tract. Am. J. Respir. Crit. Care Med. 1999; 160:1–9.