Candida-Candida and Candida-Staphylococcus species interactions in in-vitro dual-species biofilms

Bibi Khadija; Ayesha Siddiqa; Tanveer Tara; Sahar Zafar; Ujala Saba; Roohullah; Uzma Rubab; Rani Faryal

doi:10.53730/ijhs.v6nS10.14076

Authors

Bibi Khadija Department of Medical Laboratory Technology, National Skill University, Islamabad, Pakistan
Ayesha Siddiqa Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Tanveer Tara Department of Medical Laboratory Technology, National Skill University, Islamabad, Pakistan
Sahar Zafar Department of Medical Laboratory Technology, National Skill University, Islamabad, Pakistan
Ujala Saba Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Roohullah Health Laboratory, Mafraq Hospital, Abu Dhabi, United Arab Emirates | Centre of Postgraduate Studies, Lincoln University, Malaysia
Uzma Rubab Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan
Rani Faryal
ranifaryal@qau.edu.pk
Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan

Abstract

Objective: In present study, interaction between different Candida species and also their interaction with Staphylococcus species were investigated in mono and dual-species biofilm model. Methods: Resistant and weak biofilms former Candida(C. albicans, C. glabrata, C. krusi) and Staphylococcus species (S. aureus, S. epidermidis, S. saprophyticus) alongwith ATCC isolates were used in mono and different dual-species combinations for estimation of biofilms by crystal violet (CV) biofilm biomass assay and XTT reduction assay. Aspartyl proteinase activity of Candida species was also measured in these developed biofilms. Results: CV assay showed increased in biofilm biomass after 48 h of incubation in developed mono and dual-species biofilms. XTT reduction assay showed overall decreasing trend in metabolic rate in biofilms after 48 h of incubation. All three Candida species with each other in dual-species in-vitro biofilms showed antagonistic behaviour. Biofilm biomass production was raised for C. albicans and C. glabrata dual-specie biofilm with allStaphylococcus species except with clinical isolate of S. aureus and in C. glabrata-S. epidermidis dual-specie biofilm. ATCC and clinical isolate of Candida showed markedly decrease aspartyle proteinase activity when co-cultured withStaphylococcuscompared to when it was cultured alone.

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