Antibacterial efficacy of aloevera peel extract against streptococcus mutans and P. gingivalis
A preclinical experimental study
Keywords:
Antibacterial efficacy, aloevera peel extract, Streptococcus mutans, P. gingivalisAbstract
Background: The anti-inflammatory, antioxidant, antiviral, antibacterial, antitumor, antiarthritic, and anti-diabetic properties of aloe vera has been well documented in the literature. Considering its antibacterial properties evaluating its benefits as mouth rinse in the oral diseases may facilitate the patients in getting cheaper and safer herbal remedy to clean their oral cavities. Objective: To evaluate the Antibacterial efficacy of aloevera peel extract against Streptococcus mutans and P. gingivalis. Methodology: It was a preclinical experimental study conducted at Baqai Medical and Dental College Karachi, from February to March 2023. Calculated sample size was n =60. The participants were given an envelope for group randomization. S. mutant samples and P. gingivalis were incubated on appropriate media. The Aloe Vera leaves were washed and gel was removed the peels were shed dried and lastly was grinded to powder form. Ethanol (70%) were used for soaking for 15 days with intermittent shaking. The extract was diluted in distilled water in 1:4 (Extract: Distilled water) concentration.
Downloads
References
Saleem A, Naureen I, Naeem M, Murad HS, Maqsood S, Tasleem G. Aloe vera gel effect on skin and pharmacological properties. Sch Int J Anat Physiol. 2022;5(1):1-8.
Kumar A, Zechariah J, Ramchandra S, Tripathi P. Study on Aloe vera leaves farming in Rajasthan. Pharma Innov J. 2022;11(5S):289-91.
Otieno PO. A review on antiplasmodial potential and quantification of aloin and aloe-emodin in aloe vera. 2022.
Akbari B, Baghaei‐Yazdi N, Bahmaie M, Mahdavi Abhari F. The role of plant‐derived natural antioxidants in reduction of oxidative stress. BioFactors. 2022;48(3):611-33.
Prasad S, Srivastava SK. Oxidative stress and cancer: antioxidative role of Ayurvedic plants. Cancer: Elsevier; 2021. p. 301-10.
Qadeer M, Itrat N, Iftikhar N, Ahmed U. Evaluation of Hypoglycemic Impact of Aloe Vera in Diabetic Subjects. vol. 2019;4:835-8.
Martínez-Burgos WJ, Serra JL, MarsigliaF RM, Montoya P, Sarmiento-Vásquez Z, Marin O, et al. Aloe vera: From ancient knowledge to the patent and innovation landscape–A review. South African Journal of Botany. 2022.
Ebrahim AA, Elnesr SS, Abdel-Mageed M, Aly M. Nutritional significance of aloe vera (Aloe barbadensis Miller) and its beneficial impact on poultry. World's Poultry Science Journal. 2020;76(4):803-14.
Hagar S. Effect of the Infestation by Myzus persicae (Sulzer) and Tetranychus urticae Koch on the Internal Components of Aloe Vera Cactus, Aloe barbadensis (Miller). Egyptian Academic Journal of Biological Sciences A, Entomology. 2021;14(4):117-22.
Said F, Rahmawati I, Ningsih NS. Analysis of Metabolite Levels, Secondary Minerals and Aloe Vera Formulation from Kalimantan Indonesia. Medical Laboratory Technology Journal. 2022;8(1):27-34.
Hugo FN, Bailey JA, Stein C, Cunha ARd, Iser BPM, Malta DC, et al. Prevalence, incidence, and years-lived with disability due to oral disorders in Brazil: an analysis of the Global Burden of Disease Study 2019. Revista da Sociedade Brasileira de Medicina Tropical. 2022;55:e0284-2021.
Nyvad B, Takahashi N. Integrated hypothesis of dental caries and periodontal diseases. Journal of oral microbiology. 2020;12(1):1710953.
Mallya P, Mallya S. Microbiology and clinical implications of dental caries-a review. J Evol Med Dent Sci. 2020;9:3670-5.
Ji S, Kook J-K, Park S-N, Lim YK, Choi GH, Jung J-S. Characteristics of the salivary microbiota in periodontal diseases and potential roles of individual bacterial species to predict the severity of periodontal disease. Microbiology Spectrum. 2023;11(3):e04327-22.
Adelfio M, Martin‐Moldes Z, Erndt‐Marino J, Tozzi L, Duncan MJ, Hasturk H, et al. Three‐Dimensional Humanized Model of the Periodontal Gingival Pocket to Study Oral Microbiome. Advanced Science. 2023;10(12):2205473.
Amissah F, Andey T, Ahlschwede KM. Nanotechnology-based therapies for the prevention and treatment of Streptococcus mutans-derived dental caries. Journal of oral biosciences. 2021;63(4):327-36.
Kugaji MS, Kumbar VM, Peram MR, Patil S, Bhat KG, Diwan PV. Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease. Apmis. 2019;127(4):187-95.
Patil SS, Yadav AR, Chopade A, Mohite S. Design, development and evaluation of herbal mouthwash for antibacterial potency against oral bacteria. Journal of University of Shanghai for Science and Technology. 2020;22(11):881-98.1137.
Bescos R, Ashworth A, Cutler C, Brookes ZL, Belfield L, Rodiles A, et al. Effects of Chlorhexidine mouthwash on the oral microbiome. Scientific reports. 2020;10(1):5254.
Lorusso F, Tartaglia G, Inchingolo F, Scarano A. Early Response and Clinical Efficacy of a Mouthwash Containing Chlorhexidine, Anti Discoloration System, Polyvinylpyrrolidone/Vinyl Acetate and Sodium DNA in Periodontitis Model: A Triple-Blind Randomized Controlled Clinical Trial. Dentistry Journal. 2022;10(6):101.
Bagaria A, Garg V, Kumar K. Oral Hygiene The Pulse of the Practice: DENTOMED PUBLICATION HOUSE; 2022.
Deus FP, Ouanounou A. Chlorhexidine in dentistry: pharmacology, uses, and adverse effects. International dental journal. 2022;72(3):269-77.
Sonawane SK, Gokhale JS, Mulla MZ, Kandu VR, Patil S. A comprehensive overview of functional and rheological properties of aloe vera and its application in foods. Journal of Food Science and Technology. 2021;58:1217-26.
Sadiq U, Gill H, Chandrapala J. Temperature and pH Stability of Anthraquinones from Native Aloe vera Gel, Spray-Dried and Freeze-Dried Aloe vera Powders during Storage. Foods. 2022;11(11):1613.
Jangra A, Sharma G, Sihag S, Chhokar V. The dark side of miracle plant-Aloe vera: A review. Molecular Biology Reports. 2022;49(6):5029-40.
Wu J, Zhang Y, Lv Z, Yu P, Shi W. Safety evaluation of Aloe vera soft capsule in acute, subacute toxicity and genotoxicity study. PloS one. 2021;16(3):e0249356.
Abderrahmane F, Addou S, Labadie I, Kheroua O. Acute toxicity of the aqueous extract of Aloe barbadensis miller gel. Bangladesh Journal of Scientific and Industrial Research. 2023;58(1):19-34.
Polizzi E, Tetà G, Bova F, Pantaleo G, Gastaldi G, Capparà P, et al. Antibacterial properties and side effects of chlorhexidine-based mouthwashes. A prospective, randomized clinical study. Journal of Osseointegration. 2020;12(1):2-7.
Malvania EA, Sharma AS, Sheth SA, Rathod S, Chovatia NR, Kachwala MS. In vitro analysis of licorice (glycyrrhiza glabra) root extract activity on streptococcus mutans in comparison to chlorhexidine and fluoride mouthwash. J Contemp Dent Pract. 2019;20(12):1389-94.
Badri SM, Felemban EH, Alnajjar GK, Alotaibi FM, Aljahdali ST, Maher YA, et al. Effectiveness of probiotic lozenges and Chlorhexidine mouthwash on plaque index, salivary pH, and Streptococcus mutans count among school children in Makkah, Saudi Arabia. The Saudi Dental Journal. 2021;33(7):635-41.
Mensitieri F, Caggiano M, Gaudino G, Charlier B, Coglianese A, Amato A, et al. In Vitro Evaluation of Antibacterial and Antibiofilm Activity of Different Chlorhexidine-Containing Mouthwash Formulations against Streptococcus mutans. Applied Sciences. 2023;13(13):7531.
Gowtham A, Badakar C, Hugar S, Gokhale N, Davalbhakta R, Joshi R. Comparative evaluation of antibacterial efficacy of green coffee bean extract mouthwash and chlorhexidine mouthwash against Streptococcus mutans and Lactobacilli spp.-An in vitro study. Indian Journal of Health Sciences and Biomedical Research. 2020;13(2):147-.
Mehdipour A, Ehsani A, Samadi N, Ehsani M, Sharifinejad N. The antimicrobial and antibiofilm effects of three herbal extracts on Streptococcus mutans compared with Chlorhexidine 0.2%(in vitro study). Journal of Medicine and Life. 2022;15(4):526.
Joseph RA, Sabarish R, Muthukumar S, Bhat K, Balaji S. Comparative Evaluation on the effect of Herbal mouthwash on Putative Periodontal Pathogens–In vitro study. Research Journal of Pharmacy and Technology. 2023;16(1):97-102.
Yavagal PC, Rajeshwar SS. Antibacterial Efficacy of Trachyspermum ammi Oil Against Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans: An: In vitro: Study. Journal of Indian Association of Public Health Dentistry. 2022;20(4):398-402.
Barrak I, Baráth Z, Tián T, Venkei A, Gajdács M, Urbán E, et al. Effects of different decontaminating solutions used for the treatment of peri-implantitis on the growth of Porphyromonas gingivalis-an in vitro study. Acta Microbiologica et Immunologica Hungarica. 2021;68(1):40-7.
Danish P, Ali Q, Hafeez M, Malik A. Antifungal and antibacterial activity of aloe vera plant extract. Biological and Clinical Sciences Research Journal. 2020;2020(1).
Jokhio U, Buriro RS, Bughio S, Soomro SA, Arain MB, Soomro AG, et al. 4. An in-vitro antibacterial activity of Aloe vera and gentamicin against Escherichia coli and Klebsiella pneumoniae isolates from mastitis milk samples, from Tandojam, Sindh Pakistan. Pure and Applied Biology (PAB). 2021;11(2):411-20.
Naghsh N, Moghareabed A, Nematnejad M, Yaghini J, Sadeghi SM. A comparative evaluation of the antimicrobial effect of chamomile, Aloe vera-green tea, and chlorhexidine mouthwashes on some oral bacterial species. Dental Research Journal. 2023;20(1):70.
Hajiahmadi M, Faghri J, Salehi Z, Heidari F. Comparative evaluation of antibacterial effect of propolis and aloe vera, xylitol, and Cpp-Acp gels on Streptococcus mutans and Lactobacillus in vitro. International journal of dentistry. 2021;2021.
Figueiredo LC, Figueiredo NF, Cruz DFd, Baccelli GT, Sarachini GE, Bueno MR, et al. Propolis, Aloe Vera, Green Tea, Cranberry, Calendula, Myrrha and Salvia Properties against Periodontal Microorganisms. Microorganisms. 2022;10(11):2172.
Mahboub F, Nourizadeh A, Izadpanah A. The Comparison of Color Stability of Aloe Vera Gel and Chlorhexidine Solution on Acrylic Teeth. International Journal of Dentistry. 2022;2022.
Kamath DG, Nadimpalli H, Nayak SU, Rajendran V, Natarajan S. Comparison of antiplaque and anti‐gingivitis effects of aloe vera mouthwash with chlorhexidine in fixed orthodontic patients—A randomized controlled trial. International Journal of Dental Hygiene. 2023;21(1):211-8.
Published
How to Cite
Issue
Section
Copyright (c) 2023 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
