Homology modeling – a step towards vaccine development by analyzing structure of haemophilus influenza protein, transcriptional regulator H10994

Anila Farid; Madeeha Jadoon; Sofia Shoukat; Uzma Faryal; Bilal Karim; Anwar Shahzad

doi:10.53730/ijhs.v7nS1.14495

Homology modeling – a step towards vaccine development by analyzing structure of haemophilus influenza protein, transcriptional regulator H10994

https://doi.org/10.53730/ijhs.v7nS1.14495

Authors

Anila Farid Lecturer, Department of Biochemistry, Abbottabad International Medical College, Abbottabad
Madeeha Jadoon
Dr.jadoon73@gmail.com
Assistant Professor, Department of Biochemistry, Women Medical and Dental College, Abbottabad
Sofia Shoukat Assistant Professor, Department of Biochemistry, Ayub Medical College, Abbottabad
Uzma Faryal Professor and HOD, Department of Biochemistry, Women Medical and Dental College, Abbottabad
Bilal Karim Assistant Professor Biochemistry, Islamabad Medical and Dental College, Islamabad
Anwar Shahzad Associate Professor, Community Medicine and Public Health AIMI, Abbottabad

Keywords:

Haemophilus influenza, Homology modeling, vaccine development

Abstract

Introduction: Haemophilus influenza is a type of bacterium that is non motile, gram negative and causes poisoning and infection including pneumonia,bronchitis etc. In order to study the resistivity of H.influenza protein, transcription regulator: HI0433, homology modeling is an important step to predict structure. Material and methods: Bioinformatics such as CMR, BLAST, modeller Prcheck and Prosa was carried out to find 3D structure of protein. Results and Discussion: H.influenza has 1792 proteins. Out of these, 456 hypothetical proteins were found. Homology modeling of transcriptional regulator H10994 was done it consists of 8 helices and 7 beta sheets. Ramachandran plot has shown that it consists of 95.2% particles in maximum allowed regions, 2.9 % particles in fewer allowed region, 1.4% particles in inadequate allowed region, 5% particles in disallowed region. Conclusion: By homology modeling of H. influenza, transcriptional regulator protein (HI0433), structure was designed which has provided enough information for vaccine development to control its transcription for causing disease.

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Published

13-08-2023

How to Cite

Farid, A., Jadoon, M., Shoukat, S., Faryal, U., Karim, B., & Shahzad, A. (2023). Homology modeling – a step towards vaccine development by analyzing structure of haemophilus influenza protein, transcriptional regulator H10994. International Journal of Health Sciences, 7(S1), 2344–2349. https://doi.org/10.53730/ijhs.v7nS1.14495

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Issue

Vol. 7 No. S1 (2023)

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Peer Review Articles

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