A study of the effects of variety and season of collection on the amounts of bioactive compounds in Algerian olive leaves
Keywords:
Algerian cultivars, bioactive compounds, DPPH scavenger activity, ferric reducing power, Olea europaea L.Abstract
This study explored the bioactive contents (including chlorophyll, carotenoid, phenolic and flavonoid compounds) of olive leaves (Olea europaea L.) from three Algerian cultivars (Aberkane, Aharoun, and Akerma) across four distinct seasonal periods (autumn, winter, spring, and summer). The investigation involved performing antioxidant potential tests. The findings indicated that all cultivars are rich in bioactive compounds and the Aberkane olive leaves exhibited the highest levels of chlorophyll a (2518.79 ± 19.32 µg/g), chlorophyll b (816.22 µg/g), carotenoid (120.22 ± 6.72 µg/g) phenolic (596.97 ± 29.07 mg GAE/g DE) and flavonoid (374.05 ± 12.54 mg EQ/g DE) compounds. Aberkane olive leaves presented also the best results of DPPH scavenger activity (IC50: 38.40 ± 1.75 µg/ml) and ferric reducing power (A0.5: 22.34 ± 1.98 µg/ml). In general, leaves collected during summer demonstrated the highest contents of bioactive compounds and the best antioxidant activities, compared to other seasons. Consequently, it can be deduced that both the cultivar type and the season of collection exert significant influences on the bioactive content and antioxidant potential of olive leaves.
Downloads
References
Aramrueang, N., Asavasanti, S., & Khanunthong A. (2019). Leafy Vegetables. In Integrated Processing Technologies for Food and Agricultural By-Products; Pan, Z., Zhang, R., Zicari, S., Eds.; Elsevier Inc.: Amsterdam, The Netherlands, 245–272.
Arslan, D., Karabekir, Y., & Schreiner, M. (2013). Variations of phenolic compounds, fatty acids and some qualitative characteristics of Sarıulak olive oil as induced by growing area. Food Research International, 54(2), 1897-1906. https://doi.org/10.1016/j.foodres.2013.06.016
Bahloul, N., Kechaou, N., & Mihoubi, N. B. (2014). Comparative investigation of minerals, chlorophylls contents, fatty acid composition and thermal profiles of olive leaves (Olea europeae L.) as by-product. Grasas y Aceites, 65(3), e035-e035.
Benjeddou, H., Ahmed, C. B., & Rouina, B. B. (2019). Influence of antioxidative enzymes, phytohormones and pigments in alternate bearing of three olive cultivars. Scientia Horticulturae, 253, 17-23. https://doi.org/10.1016/j.scienta.2019.04.036
Brahmi, F., Mechri, B., Dabbou, S., Dhibi, M., & Hammami, M. (2012). The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations. Industrial Crops and Products, 38, 146-152. https://doi.org/10.1016/j.indcrop.2012.01.023
Bulda, O. V., Rassadina, V. V., Alekseichuk, H. N., & Laman, N. A. (2008). Spectrophotometric measurement of carotenes, xanthophylls, and chlorophylls in extracts from plant seeds. Russian Journal of Plant Physiology, 55, 544-551.’
Burits, M., & Bucar, F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy research, 14(5), 323-328.
Carchi, J. A. Y. ., Catagua, T. C. M. ., Rivera, D. G. B. ., Mera, V. B. ., & Rosario, M. del . (2021). From beginner to expert, experience of the rotating nursing intern in pre-professional practice. International Journal of Health Sciences, 5(2), 111–117. https://doi.org/10.29332/ijhs.v5n2.1291
Casida, J. E. (2009). Pest toxicology: the primary mechanisms of pesticide action. Chemical research in toxicology, 22(4), 609-619.
Chinnici, F., Bendini, A., Gaiani, A., & Riponi, C. (2004). Radical scavenging activities of peels and pulps from cv. Golden Delicious apples as related to their phenolic composition. Journal of Agricultural and food chemistry, 52(15), 4684-4689.
Domonkos, I., Kis, M., Gombos, Z., & Ughy, B. (2013). Carotenoids, versatile components of oxygenic photosynthesis. Progress in lipid research, 52(4), 539-561. https://doi.org/10.1016/j.plipres.2013.07.001
Garcia-Vaquero, M., & Rajauria, G. (2018). Analytical techniques for phytochemical estimation in fruit juices. In Fruit Juices (pp. 669-692). Academic Press. https://doi.org/10.1016/B978-0-12-802230-6.00033-3
Goulas, V., Papoti, V. T., Exarchou, V., Tsimidou, M. Z., & Gerothanassis, I. P. (2010). Contribution of flavonoids to the overall radical scavenging activity of olive (Olea europaea L.) leaf polar extracts. Journal of Agricultural and Food Chemistry, 58(6), 3303-3308.
Gullon, B., Eibes, G., Moreira, M. T., Herrera, R., Labidi, J., & Gullon, P. (2018). Yerba mate waste: A sustainable resource of antioxidant compounds. Industrial crops and products, 113, 398-405. https://doi.org/10.1016/j.indcrop.2018.01.064
Halliwel, B. (1992). Free radicals, antioxidants and human disease. Where are we now?. J Lab Clin Med, 119, 598-620.
Hayes, J. E., Allen, P., Brunton, N., O’grady, M. N., & Kerry, J. P. (2011). Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical products: Olive leaf extract (Olea europaea L.), lutein, sesamol and ellagic acid. Food Chemistry, 126(3), 948-955. https://doi.org/10.1016/j.foodchem.2010.11.092
Indrasti, D., Andarwulan, N., Purnomo, E. H., & Wulandari, N. U. R. (2018). Stability of chlorophyll as natural colorant: A review for suji (Dracaena Angustifolia Roxb.) leaves’ case. Current Research in Nutrition and Food Science Journal, 6(3), 609-625.
Jain, D. P., Pancholi, S. S., & Patel, R. (2011). Synergistic antioxidant activity of green tea with some herbs. Journal of advanced pharmaceutical technology & research, 2(3), 177.
Khaliq, A., Sabir, S. M., Ahmad, S. D., Boligon, A. A., Athayde, M. L., Jabbar, A., ... & Khan, A. (2015). Antioxidant activities and phenolic composition of Olive (Olea europaea) leaves. J Appl Bot Food Qual, 88, 16-21.
Lanfer-Marquez, U. M., Barros, R. M., & Sinnecker, P. (2005). Antioxidant activity of chlorophylls and their derivatives. Food research international, 38(8-9), 885-891. https://doi.org/10.1016/j.foodres.2005.02.012
Lee, O. H., & Lee, B. Y. (2010). Antioxidant and antimicrobial activities of individual and combined phenolics in Olea europaea leaf extract. Bioresource technology, 101(10), 3751-3754. https://doi.org/10.1016/j.biortech.2009.12.052
Medina, E., Romero, C., Brenes, M., García, P., de Castro, A., & García, A. (2008). Profile of anti-lactic acid bacteria compounds during the storage of olives which are not treated with alkali. European Food Research and Technology, 228, 133-138.
Middleton Jr, E. (1998). Effect of plant flavonoids on immune and inflammatory cell function. Flavonoids in the living system, 175-182.
Mokrani, A., & Madani, K. (2016). Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology, 162, 68-76. https://doi.org/10.1016/j.seppur.2016.01.043
Moudache, M., Colon, M., Nerín, C., & Zaidi, F. (2016). Phenolic content and antioxidant activity of olive by-products and antioxidant film containing olive leaf extract. Food Chemistry, 212, 521-527. https://doi.org/10.1016/j.foodchem.2016.06.001
Nagata, M., & Yamashita, I. (1992). Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Nippon shokuhin kogyo gakkaishi, 39(10), 925-928.
Oyaizu, M. (1986). Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese journal of nutrition and dietetics, 44(6), 307-315.
Papoti, V. T., Papageorgiou, M., Dervisi, K., Alexopoulos, E., Apostolidis, K., & Petridis, D. (2018). Screening olive leaves from unexploited traditional Greek cultivars for their phenolic antioxidant dynamic. Foods, 7(12), 197.
Rahmanian, N., Jafari, S. M., & Wani, T. A. (2015). Bioactive profile, dehydration, extraction and application of the bioactive components of olive leaves. Trends in Food Science & Technology, 42(2), 150-172. https://doi.org/10.1016/j.tifs.2014.12.009
Rietjens, S. J., Bast, A., & Haenen, G. R. (2007). New insights into controversies on the antioxidant potential of the olive oil antioxidant hydroxytyrosol. Journal of agricultural and food chemistry, 55(18), 7609-7614.
Romero, C., Medina, E., Mateo, M. A., & Brenes, M. (2017). Quantification of bioactive compounds in Picual and Arbequina olive leaves and fruit. Journal of the Science of Food and Agriculture, 97(6), 1725-1732.
Sarbishegi, M., Gorgich, E. A. C., & Khajavi, O. (2017). Olive leaves extract improved sperm quality and antioxidant status in the testis of rat exposed to rotenone. Nephro-Urology Monthly, 9(3).
Sass-Kiss, A., Kiss, J., Milotay, P., Kerek, M. M., & Toth-Markus, M. (2005). Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International, 38(8-9), 1023-1029. https://doi.org/10.1016/j.foodres.2005.03.014
Schoefs, B. (2003). Chlorophyll and carotenoid analysis in food products. A practical case-by-case view. TrAC Trends in Analytical Chemistry, 22(6), 335-339. https://doi.org/10.1016/S0165-9936(03)00602-2
Sevim, D., & Tuncay, Ö. (2012). Ayvalik ve Memecik zeytin Çeşitlerinin yapraği ve meyvelerinin toplam fenolik madde miktarı ve antioksidan Aktiviteleri. Gıda, 37(4), 219-226.
Shahidi, F. (Ed.). (1997). Natural antioxidants: chemistry, health effects, and applications. The American Oil Chemists Society.
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.
Somogyi, A., Rosta, K., Pusztai, P., Tulassay, Z., & Nagy, G. (2007). Antioxidant measurements. Physiological measurement, 28(4), R41.
Sudhakar, P., Latha, P., & Reddy, P. V. (2016). Phenotyping crop plants for physiological and biochemical traits. Academic Press.
Talhaoui, N., Taamalli, A., Gómez-Caravaca, A. M., Fernández-Gutiérrez, A., & Segura-Carretero, A. (2015). Phenolic compounds in olive leaves: Analytical determination, biotic and abiotic influence, and health benefits. Food Research International, 77, 92-108. https://doi.org/10.1016/j.foodres.2015.09.011
Tarchoune, I., Sgherri, C., Eddouzi, J., Zinnai, A., Quartacci, M. F., & Zarrouk, M. (2019). Olive leaf addition increases olive oil nutraceutical properties. Molecules, 24(3), 545.
Trees, C. C., Clark, D. K., Bidigare, R. R., Ondrusek, M. E., & Mueller, J. L. (2000). Accessory pigments versus chlorophyll a concentrations within the euphotic zone: A ubiquitous relationship. Limnology and Oceanography, 45(5), 1130-1143.
Wainstein, J., Ganz, T., Boaz, M., Bar Dayan, Y., Dolev, E., Kerem, Z., & Madar, Z. (2012). Olive leaf extract as a hypoglycemic agent in both human diabetic subjects and in rats. Journal of medicinal food, 15(7), 605-610.
Wang, X., Li, C., Liu, Y., Li, H., & Di, D. (2011). Efficient method for screening and identification of radical scavengers in the leaves of Olea europaea L. Biomedical Chromatography, 25(3), 373-380.
Published
How to Cite
Issue
Section
Copyright (c) 2024 International journal of health sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.