In-vitro evaluation of antimicrobial efficacy of triple antibiotic paste, metapex and newly introduced iodine based asphaline temp as intracanal medicament against enterococcus faecalis

Gaurav Jain; Srinkhala Sharma; Shatakshi Rastogi; Balakrishnan Rajkumar; Lalit C. Boruah

doi:10.53730/ijhs.v6nS1.4807

Authors

Gaurav Jain
gauravjs23@yahoo.com
Associate Professor, Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow, India– 227105
Srinkhala Sharma MDS, Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow, India
Shatakshi Rastogi MDS, Department of Conservative Dentistry and Endodontics, Saraswati Dental College, Lucknow, India
Balakrishnan Rajkumar Principal, Professor and Head of Department, Department of Conservative Dentistry and Endodontics, BBD College of Dental Sciences, Lucknow, India
Lalit C. Boruah Reader, Department of Conservative Dentistry and Endodontics, Government Dental College, Dibrugarh, India

Keywords:

asphaline temp, calcium hydroxide, colony-forming units, intra-canal medicament, triple antibiotic paste

Abstract

Background: Microorganisms and their products are considered as the etiological agents of pulp necrosis and peri-radicular lesions. They may survive during endodontic procedures due to anatomical structural complexities and limitations of access by instrumentation and irrigants. The present study was conducted to assess antimicrobial efficacy of newly introduced iodine based Asphaline Temp, Triple antibiotic paste and Calcium hydroxide based Metapex against Enterococcus faecalis. Materials & Methods: 90 extracted, single-rooted human permanent teeth were instrumented and autoclaved. Samples were inoculated with the pure culture of E. faecalis and incubated. After incubation, colony-forming units (CFUs) were recorded before medication. Each group was further divided into 3 subgroups based on time duration of testing for days – 1 day, 5 days, and 7 days, containing 10 samples each. Group I – Asphaline Temp; Group II – Triple antibiotic paste (TAP); Group III – Metapex. The various time interval bacterial CFUs within the group were compared. Results: Percentage reduction in bacteria count on 1 day, 5 day and 7 day in Group I (Asphaline Temp) was 77.2, 96.5 and 98.3; in Group II (TAP) was 70.5, 90.4 and 93.2 and in Group III (Metapex ) was 25.8, 46.2 and 58.2.

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