Evaluation and comparison of efficacy of different storage media in maintaining the viability of periodontal ligament cells-in vitro study

Patel Mahendra; Batra Manali; Kesharani Pooja; Mistry Kunjal; Trivedi Vaidehi; Makwana Sawan

doi:10.53730/ijhs.v6nS1.5297

Authors

Patel Mahendra
drmahenpatel@yahoo.com
Professor and Head, Department of Conservative dentistry and Endodontics, College of Dental Sciences and Research Centre
Batra Manali M.D.S Conservative Dentistry and Endodontics
Kesharani Pooja Reader, Conservative dentistry and Endodontics, College of Dental Sciences and Research Centre
Mistry Kunjal Associate Professor, Conservative dentistry and Endodontics, Dharamsinh Desai University, Nadiad, Gujarat
Trivedi Vaidehi Post graduate student, Department of Conservative dentistry and Endodontics, College of Dental Sciences and Research Centre
Makwana Sawan Post graduate student, Department of Conservative dentistry and Endodontics, College of Dental Sciences and Research Centre

Keywords:

Avulsion, Aloe Vera Gel, Green Tea Extract, HBSS, Viaspan Periodontal ligament

Abstract

Aim: Aim of the study is evaluate the efficacy of HBSS, Green tea extract and Aloe Vera gel as storage media in maintaining the viability of periodontal ligament cell. Material and Method: Fifty- five human teeth with closed apices and with apparently normal periodontium were extracted as atraumatically as possible for orthodontic reasons were collected for study. Teeth were randomly divided into positive control group, negative control group, three experimental group. Teeth in experimental groups were dried for 30 minutes (including time taken for curetting coronal PDL cells), followed by a 45-minute immersion in one of the three storage solution groups. The teeth in positive control group after extraction was immediately treated with collagenase. The teeth in negative control group were bench- dried for 8 hours, with no follow- up storage solution time, and then placed in the collagenase. Result: HBSS is the best medium for the maintaining the viability of the PDL Cells.But easier availability and cost effectiveness, GTE and Aloe Vera Gel can be advocated as a viable storage medium. Conclusion: It can be concluded that GTE and Aloe Vera Gel can be advocated as a viable storage medium.

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