Comparing the effectiveness of aqueous and alcoholic rubia cordifolia extracts against streptococcus mutans and lactobacillus acidophilus with chlorhexidine: An in vitro study

Sathya Kumaresan; Pradeep Kumar Rathinavelu; Arthi Balasubramaniam

doi:10.53730/ijhs.v6nS1.5310

Authors

Sathya Kumaresan Post graduate, Department of Public Health Dentistry,Saveetha Dental college, Saveetha Institute of Medical and Technical Sciences, Saveetha University No.162, Poonamallee high road,Chennai 600077, Tamil Nadu, India
Pradeep Kumar Rathinavelu
pradeepkumarr.sdc@saveetha.com
Professor and Head, Department of Public Health Dentistry, Saveetha Dental college, Saveetha Institute of Medical and Technical Sciences, Saveetha University No.162, Poonamallee high road,Chennai 600077, Tamil Nadu, India
Arthi Balasubramaniam Senior Lecturer, Department of Public Health Dentistry, Saveetha Dental college, Saveetha Institute of Medical and Technical Sciences,No.162, Poonamallee high road, Chennai 600077, Tamil Nadu, India

Keywords:

alcoholic extract, aqueous extract, chlorhexidine, r.cordifolia, streptococcus mutans

Abstract

Introduction: Because of the majorly reported undesirable effects of excessive antibiotic usage like drug hypersensitivity and drug virulence, herbal extracts serve as alternatives with potent therapeutic effects and lesser side effects. The aim of the current study was to evaluate the effectiveness of aqueous and alcoholic Rubia cordifolia extracts against S.mutans and L.acidophilus. Materials and Methods: The antibacterial activity of aqueous and alcoholic extracts of R.cordifolia was tested on S.mutans and L.acidophilus and compared with the gold standard Chlorhexidine. Aqueous extract and alcoholic extracts of R.cordifolia was formulated.The antibacterial activity of R.cordifolia was determined by agar well diffusion method. Results: The aqueous extract of R.cordifolia showed a higher zone of inhibition for L.acidophilus at 100 μl measuring 21mm. The alcoholic extract of R.cordifolia showed a higher zone of inhibition for S. mutans which was the maximum at 100 μl measuring 21mm. The zone of inhibition of Aqueous and Alcoholic extracts of R.cordifolia was much superior to chlorhexidine against L.acidophilus and S.mutans respectively. Conclusion: The aqueous and alcoholic Rubia cordifolia extracts were effective against S.mutans and L.acidophilus. Aqueous extract of R.cordifolia is superior in inhibiting l.acidophilus than chlorhexidine. Alcoholic extracts of R.cordifolia is superior in inhibiting s.mutans than chlorhexidine.

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References

Ajagannanavar SL, Battur H, Shamarao S, Sivakumar V, Patil PU, Shanavas P.(2014). Effect of aqueous and alcoholic licorice (glycyrrhiza glabra) root extract against streptococcus mutans and lactobacillus acidophilus in comparison to chlorhexidine: an in vitro study. J Int Oral Health. Jul;6(4):29–34.

Abdollahzadeh S, Mashouf R, Mortazavi H, Moghaddam M, Roozbahani N, Vahedi M.( 2011 ). Antibacterial and antifungal activities of punica granatum peel extracts against oral pathogens. J Dent . Mar 31;8(1):1–6.

Kshirsagar MM, Dodamani AS, Karibasappa GN, Vishwakarma PK, Vathar JB, Sonawane KR, et al.(2018). Antibacterial activity of garlic extract on cariogenic bacteria: An study. Ayu. Jul;39(3):165–8.

Bowden GHW. (2000).The Microbial Ecology of Dental Caries. Vol. 12, Microbial Ecology in Health & Disease. doi.org/10.3402/mehd.v12i3.8194

Chismirina S, Sungkar S, Andayani R, Rezeki S, Darmawi.(2019). Existence of Streptococcus Mutans and Streptococcus Sobrinus in Oral Cavity as Main Cariogenc Bacteria of Dental Caries Proceedings of the 1st Aceh International Dental Meeting (AIDEM 2019), Oral Health International Conference On Art, Nature And Material Science Development.doi.org/10.2991/ahsr.k.210201.020

Grigalauskienė R, Slabšinskienė E, Vasiliauskienė I.(2015). Biological approach of dental caries management. Stomatologija.17(4):107–12.

Sharma M, Tandon S, Aggarwal V, Bhat KG, Kappadi D, Chandrashekhar P, et al. (2015).Evaluation of antibacterial activity of Calotropis gigentica against Streptococcus mutans and Lactobacillus acidophilus: An in vitro comparative study. J Conserv Dent.Nov;18(6):457–60.

Kamble S, Humbare R, Sarkar J, Kulkarni A. (2020).Assessment of Phytochemicals and Antioxidant Properties of Root Extracts of Rubia cordifolia L. in Different Solvent Systems. Proceedings of The 1st International Electronic Conference on Plant Science. doi.org/10.3390/iecps2020-08625

Ramesh A, Varghese SS, Doraiswamy JN, Malaiappan S.(2016). Herbs as an antioxidant arsenal for periodontal diseases. J Intercult Ethnopharmacol. Jan;5(1):92–6.

Menezes SMS, Cordeiro LN, Viana GSB. (2006).Punica granatum(Pomegranate) Extract Is Active Against Dental Plaque Vol. 6, Journal of Herbal Pharmacotherapy. p. 79–92. doi.org/10.1080/j157v06n02_07

Chowdhury U, Baruah PK.(2020). Betelvine (Piper betle L.): A potential source for oral care [Internet]. Current Botany. p. 87–92. doi.org/10.25081/cb.2020.v11.6130

Mehta VV, Rajesh G, Rao A, Shenoy R, B H MP.(2014). Antimicrobial Efficacy of Punica granatum mesocarp, Nelumbo nucifera Leaf, Psidium guajava Leaf and Coffea Canephora Extract on Common Oral Pathogens: An In-vitro Study. J Clin Diagn Res. Jul;8(7):ZC65–8.

Ellepola ANB, Samaranayake LP. (2001).Adjunctive use of chlorhexidine in oral candidoses: a review [Internet]. Vol. 7, Oral Diseases. 2001. p. 11–7. doi.org/10.1034/j.1601-0825.2001.70103.x

Ajagannanavar SL, Shamarao S, Battur H, Tikare S, Al-Kheraif AA, Al Sayed MSAE.(2014). Effect of aqueous and alcoholic Stevia (Stevia rebaudiana) extracts against Streptococcus mutans and Lactobacillus acidophilus in comparison to chlorhexidine: An in vitro study. J Int Soc Prev Community Dent. Dec;4(Suppl 2):S116–21.

Basu S, Ghosh A, Hazra B.(2005). Evaluation of the antibacterial activity of Ventilago madraspatana Gaertn., Rubia cordifolia Linn. and Lantana camara Linn.: isolation of emodin and physcion as active antibacterial agents. Phytother Res. Oct;19(10):888–94.