Cytotoxicity and antimicrobial activity of black cumin reinforced with hydroxyapatite crystals by green synthesis process
An invitro study
Keywords:
black cumin, green synthesis, hydroxyapatite crystals nigella sativaAbstract
Introduction: Nigella sativa L. is an herb belonging to the Ranunculaceae family that grows in countries bordering the Mediterranean Sea. They are traditional Arab herbal medicines. The seed of N. sativa (black cumin or Habbatul Barakah) reported to have antibacterial, antifungal, antitumor, antihypertensive, antioxidant, hepatoprotective histamine release inhibitors. The recent development in the field of nanotechnology is by the imminent application of nano-hydroxyapatite in dentistry for remineralization of enamel. The aim of the study is to incorporate the black cumin seed extract with hydroxyapatite crystals into a new formulation for the first time to evaluate the antimicrobial and cytotoxic properties and to characterize their structural and morphological properties. Materials and method: The organisms used were Streptococcus mutans, Staphylococcus aureus, E. Faecalis and Candida albicans. Agar well diffusion method was used to assess the antimicrobial efficacy of the nanoparticles at various concentrations that ranges from 25 µL, 50 µL and 100 µL. The synthesized nanoparticles were characterized by using ultraviolet double beam spectrophotometers in the wavelength range of 250-750nm. Results: Zone of inhibition was found to be highest at 100 µL concentration against Staphylococcus mutans and Candida albicans.
Downloads
References
Moghadam ET, Yazdanian M, Tahmasebi E, Tebyanian H, Ranjbar R, Yazdanian A, et al. Current herbal medicine as an alternative treatment in dentistry: In vitro, in vivo and clinical studies. Eur J Pharmacol. 2020 Oct 21;173665.
Kouidhi B, Zmantar T, Jrah H, Souiden Y, Chaieb K, Mahdouani K, et al. Antibacterial and resistance-modifying activities of thymoquinone against oral pathogens. Ann Clin Microbiol Antimicrob. 2011 Jun 27;10:29.
Al-Attass SA, Zahran FM, Turkistany SA. Nigella sativa and its active constituent thymoquinone in oral health. Saudi Med J. 2016 Mar;37(3):235–44.
Ozdemir H, Kara MI, Erciyas K, Ozer H, Ay S. Preventive effects of thymoquinone in a rat periodontitis model: a morphometric and histopathological study [Internet]. Vol. 47, Journal of Periodontal Research. 2012. p. 74–80. Available from: http://dx.doi.org/10.1111/j.1600-0765.2011.01406.x
Pepla E, Besharat LK, Palaia G, Tenore G, Migliau G. Nano-hydroxyapatite and its applications in preventive, restorative and regenerative dentistry: a review of literature. Ann Stomatol . 2014 Jul;5(3):108–14.
Coelho CC, Grenho L, Gomes PS, Quadros PA, Fernandes MH. Nano-hydroxyapatite in oral care cosmetics: characterization and cytotoxicity assessment. Sci Rep. 2019 Jul 30;9(1):11050.
Garazzino S, Lutsar I, Bertaina C, Tovo P-A, Sharland M. New antibiotics for paediatric use: A review of a decade of regulatory trials submitted to the European Medicines Agency from 2000—Why aren’t we doing better? [Internet]. Vol. 42, International Journal of Antimicrobial Agents. 2013. p. 99–118. Available from: http://dx.doi.org/10.1016/j.ijantimicag.2013.05.001
Singh M, Manikandan S, Kumaraguru AK. Nanoparticles: A New Technology with Wide Applications [Internet]. Vol. 1, Research Journal of Nanoscience and Nanotechnology. 2011. p. 1–11. Available from: http://dx.doi.org/10.3923/rjnn.2011.1.11
Safithri F, Andriani K. Black Cummin Extract (Nigella sativa Linn) on Spatial Memory Performance in Menopausal Model of Rat [Internet]. Proceedings of the Health Science International Conference (HSIC 2017). 2017. Available from: http://dx.doi.org/10.2991/hsic-17.2017.40
Fathiyah S, Taat PS, Ferdiansyah F. Nigella sativa L seed’s Extract Modulates Liver Regeneration by Affecting Endogenous Stem Cells in Liver Fibrosis Model of Rat [Internet]. Proceedings of the 1st International Conference in One Health (ICOH 2017). 2018. Available from: http://dx.doi.org/10.2991/icoh-17.2018.11
Nasuti C, Bordoni L, Fedeli D, Gabbianelli R. Effect of Nigella sativa Oil in a Rat Model of Adjuvant-Induced Arthritis [Internet]. Vol. 11, Proceedings. 2019. p. 16. Available from: http://dx.doi.org/10.3390/proceedings2019011016
Harzallah HJ, Kouidhi B, Flamini G, Bakhrouf A, Mahjoub T. Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone [Internet]. Vol. 129, Food Chemistry. 2011. p. 1469–74. Available from: http://dx.doi.org/10.1016/j.foodchem.2011.05.117
Rahman M, Wadud M, Islam T, Hussain M, Bristy E, Tuhin A. Evaluation of Antibacterial Activity of Piper betel Leaves and Nigella sativa Seeds against Multidrug Resistant Food and Water Borne Pathogenic Bacteria: An in vitro Study Model [Internet]. Vol. 22, Microbiology Research Journal International. 2018. p. 1–11. Available from: http://dx.doi.org/10.9734/mrji/2017/37796
Epand RM. Host Defense Peptides and Their Potential as Therapeutic Agents. Springer; 2016. 321 p.
Randhawa MA, Gondal MA, Al-Zahrani A-HJ, Rashid SG, Ali A. Synthesis, morphology and antifungal activity of nano-particulated amphotericin-B, ketoconazole and thymoquinone against Candida albicans yeasts and Candida biofilm. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2015;50(2):119–24.
Khan MA, Aldebasi YH, Alsuhaibani SA, AlSahli MA, Alzohairy MA, Khan A, et al. Therapeutic potential of thymoquinone liposomes against the systemic infection of Candida albicans in diabetic mice. PLoS One. 2018 Dec 27;13(12):e0208951.
Al-Thobity AM, Al-Khalifa KS, Gad MM, Al-Hariri M, Ali AA, Alnassar T. In Vitro Evaluation of the Inhibitory Activity of Thymoquinone in Combating Candida albicans in Denture Stomatitis Prevention. Int J Environ Res Public Health [Internet]. 2017 Jul 8;14(7). Available from: http://dx.doi.org/10.3390/ijerph14070743
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
