Cytotoxicity and antimicrobial activity of black cumin reinforced with hydroxyapatite crystals by green synthesis process
An invitro study
Keywords:
black cumin, green synthesis, hydroxyapatite crystals nigella sativaAbstract
Introduction: Nigella sativa L. is an herb belonging to the Ranunculaceae family that grows in countries bordering the Mediterranean Sea. They are traditional Arab herbal medicines. The seed of N. sativa (black cumin or Habbatul Barakah) reported to have antibacterial, antifungal, antitumor, antihypertensive, antioxidant, hepatoprotective histamine release inhibitors. The recent development in the field of nanotechnology is by the imminent application of nano-hydroxyapatite in dentistry for remineralization of enamel. The aim of the study is to incorporate the black cumin seed extract with hydroxyapatite crystals into a new formulation for the first time to evaluate the antimicrobial and cytotoxic properties and to characterize their structural and morphological properties. Materials and method: The organisms used were Streptococcus mutans, Staphylococcus aureus, E. Faecalis and Candida albicans. Agar well diffusion method was used to assess the antimicrobial efficacy of the nanoparticles at various concentrations that ranges from 25 µL, 50 µL and 100 µL. The synthesized nanoparticles were characterized by using ultraviolet double beam spectrophotometers in the wavelength range of 250-750nm. Results: Zone of inhibition was found to be highest at 100 µL concentration against Staphylococcus mutans and Candida albicans.
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