Phytochemical constituents and anti-inflammatory activities of Clerodendrum petasites

Thidarut Kwuansawat; Waraporn Putalun; Wongwiwat Tassaneeyakul; Pramote Mahakunakorn

doi:10.53730/ijhs.v6nS1.5542

Authors

Thidarut Kwuansawat Applied Thai Traditional Medicine, Faculty of Medicine, Mahasarakham University, Maha and Graduate School, Khon Kaen University, Khon Kaen, Thailand
Waraporn Putalun Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
Wongwiwat Tassaneeyakul Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
Pramote Mahakunakorn
pramah@kku.ac.th
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand

Keywords:

Clerodendrum petasites, inflammation, phytochemicals, phenolic compounds, medicinal plants

Abstract

Inflammation is process of immune responses to microbial infections and cell damage. Macrophages are pro-inflammatory leukocytes crucial for initiation, maintenance, and resolution of inflammation. Root extract of a medicinal plant Clerodendrum petasites (CP), has been widely used as a component of antipyretic and anti-inflammatory remedies. In the present study, we investigated whether CP aerial part (leaf and stem) extracts had the same contents with the root extracts as well as their cytotoxicity and inflammatory effects in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 monocyte/macrophage-like cell model. Analysis of the methanol-extracted CP contents with the high-performance liquid chromatography ultra-violet spectroscopy (HPLC-UV) showed that major phenolic compounds found in the root extract were verbascoside and quercetin, while the aerial parts also additionally contained rosmarinic acid and hispidulin. These CP extracts were not toxic to the cells and able to inhibit production of pro-inflammatory markers, nitric oxide (NO) and prostaglandin E2 (PGE2). In addition, the aerial CP extracts also inhibited the pro-inflammatory cytokines, interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α), suggesting additional role of rosmarinic acid and hispidulin. In conclusion, this study showed that the aerial parts of the CP contained phytochemical constituents and could be used as anti-inflammatory medicinal plants.

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