Effectiveness of magnetic field in stimulation of biochemical and enzymes activities in seedling and callus of Nigella sativa

Sawsan J. A. AL-Allaf; Rehab A. H. AL-Baker

doi:10.53730/ijhs.v6nS2.5818

Authors

Sawsan J. A. AL-Allaf Dep. Of Biology/ College of Science/ University of Mosul
Rehab A. H. AL-Baker
rehsbio39@uomosul.edu.iq
Dep. Of Environmental Sciences/ College of Environmental and Technologies/ University of Mosul

Keywords:

magnetic field, callus, nigella sativa, antioxidant

Abstract

This current study investigated the influence of Magnetic Field from Winding coil magnet about ( 400 mT ) for different exposure periods ( 0, 30 , 60 , 90 , 120 , 150 min ) on seed germination, callus initiation, and callus fresh weight, protein, carbohydrate and proline content and vitality , in addition to the efficacy of some antioxidant enzymes . The results indicated that the highest seed germination percentage was when exposed for an (90 Minutes). Also found that the best exposure period for acceleration of callus initiation was (30 minutes). Fresh weight, protein, proline, carbohydrates content and callus vitality got a better result under (90 minutes) of magnetic field exposure . On the other hand, the antioxidant enzymes activity significantly increased with 150 minutes M.F treatment.

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References

Abd-Elbaset, M., Arafa, E. S. A., El Sherbiny, G. A., Abdel- Bakky, M. S., & Elgendy, A. N. A. (2017). Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway. Naunyn-Schmiedeberg's archives of pharmacology, 390(1), 69-76.

Abdollahi, F., Amiri, H., Niknam, V., Ghanati, F., & Mahdigholi,

Al-Bakr, Rehab Abdel-Jabbar Hamid (2002) The role of some standard and synthetic growth regulators recently in the development and growth of callus from the plant L. sativa Nigella and the level of active Ingredients. Master Thesis, Public College, University of Mosul, Iraq.

Almoyad, M. (2018). Synergism from combination of targeted therapy and phytochemicals in colorectal cancer.

Almshawit, H., & Macreadie, I. (2017). Fungicidal effect of thymoquinone involves generation of oxidative stress in Candida glabrata. Microbiological research, 195, 81-88.

Anand A., Nagarajan S., Verma A. et al.: Pre-treatment of seeds with static magnetic field ameliorates soil water stress in seedlings of maize (Zea mays L.). — Indian J. Biochem. Biophys. 49: 63– 70, 2012.

Anwar, Y., Ullah, I., Al Johny, B. O., Al-Shehri, A. M., Bakhsh, E. M., Ul-Islam, M., ... & Kamal, T. (2021). Nigella sativa L. seeds extract assisted synthesis of silver nanoparticles and their antibacterial and catalytic performance. Applied Nanoscience, 1- 12.

Asghar, T., Jamil, Y., Iqbal, M., & Abbas, M. (2016). Laser light and magnetic field stimulation effect on biochemical, enzymes activities and chlorophyll contents in soybean seeds and seedlings during early growth stages. Journal of Photochemistry and Photobiology B: Biology, 165, 283-290.

Baghel, L., Kataria, S., & Guruprasad, K. N. (2018). Effect of static magnetic field pretreatment on growth, photosynthetic performance and yield of soybean under water stress. Photosynthetica, 56(2), 718-730.

Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39(1), 205-207.

Baxter, A., Mittler, R., & Suzuki, N. (2014). ROS as key players in plant stress signalling. Journal of experimental botany, 65(5), 1229-1240.

Belyavskaya, N. A. (2001). Ultrastructure and calcium balance in meristem cells of pea roots exposed to extremely low magnetic fields. Advances in Space Research, 28(4), 645-650.

Cakmak, T., Dumlupinar, R. and Erdal, S. 2010. Acceleration of germination and early growth of wheat and bean seedlings grown under various magnetic field and osmotic conditions. Bioelectromagnetics, 30: 1-10.

Chandran, H., Meena, M., Barupal, T., & Sharma, K. (2020). Plant tissue culture as a perpetual source for production of industrially important bioactive compounds. Biotechnology Reports, 26, e00450.

Chaudhry, Z.; Khera, R.A.; Hanif, M.A.; Ayub, M.A.; Sumrra,

E. D. H. (2018). Protective effect of Nigella sativa on 4- nonylphenol-induced nephrotoxicity in Clarias gariepinus (Burchell, 1822). Science of the Total Environment, 619, 692-699.

Ekramian, S., Abbaspour, H., Roudi, B., Amjad, L., & Nafchi, A.

Erol, B. Ü. L. E. N. T., Sari, U., Amasyali, A. S., Ozkanli, S.,

Golbaz, G., & Kaviani, B. (2019). Effect of Magnetic Field on Growth and Development Parameters of Rudbeckia hirta L. Seed in Dry and Humid Conditions. Journal of Ornamental Plants, 9(4), 233-243

Goth, L.(1991). A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta,196 (2-3): 143-152.

Hassanpour, H., Mansourkhaki, M., & Hekmati, M. (2019). Effect of static magnetic field on the growth factors, antioxidant activity and anatomical responses of Silybum marianum seedlings. Journal of Plant Process and Function, 7(28), 9-15.

Herbert, D., Phipps, P. J., & Strange, R. E. (1971). Chapter III chemical analysis of microbial cells. In Methods in microbiology (Vol. 5, pp. 209-344). Academic press.

Javed N., Ashraf M., Akram N., Al-Qurainy F.: Alleviation of adverse effects of drought stress on growth and some potential physiological attributes in maize (Zea mays) by seed electromagnetic treatment. — Photochem. Photobiol. 87: 1354– 1362, 2011.

K. (2019). Effects of static magnetic fields on the antioxidant system of almond seeds. Russian Journal of Plant Physiology, 66(2), 299-307.

Kargarshooraki, E. and Majd, A. 2016. The comparative study of the electromagnetic fields’ effects on seed germination, growth and development indicators of Nigella sativa L. seeds. Journal of Plant Research, 29 (4): 867-873 (In Persian ).

Kaur, G., & Asthir, B. J. B. P. (2015). Proline: a key player in plant abiotic stress tolerance. Biologia plantarum, 59(4), 609-619.

Kim, Y.H. and Yaoo,J.Y.(1996). Peroxidase production from carrot hairy root cell culture.Enzyme and Microbial Technology, 18(7); 531-535.

Kotb, A. M., Abd-Elkareem, M., Abou Khalil, N. S., & Sayed, A.

Latef, A. A. H. A., Dawood, M. F., Hassanpour, H., Rezayian, M., & Younes, N. A. (2020). Impact of the static magnetic field on growth, pigments, osmolytes, nitric oxide, hydrogen sulfide, phenylalanine ammonia-lyase activity, antioxidant defense system, and yield in lettuce. Biology, 9(7).

Liang, C., Wu, R., Han, Y., Wan, T., & Cai, Y. (2019). Optimizing suitable antibiotics for bacterium control in micropropagation of cherry rootstock using a modified leaf disk diffusion method and E test. Plants, 8(3), 66.

M. (2021). Influence of Nigella sativa L. Extract on Physico‒ Mechanical and Antimicrobial Properties of Sago Starch Film. Journal of Polymers and the Environment, 29(1), 201-208.

Maffei, M. E. (2014). Magnetic field effects on plant growth, development, and evolution. Frontiers in plant science, 5, 445.

Marshall, S. V. and GG Skitek 1987. Electromagnetic Concepts and Applications.

McQueen-Mason, S., & Cosgrove, D. J. (1994). Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension. Proceedings of the National Academy of Sciences, 91(14), 6574-6578.

Mroczek-Zdyrska, M., Tryniecki, Ł., Kornarzyński, K., Pietruszewski, S., & Gagoś, M. (2021). Influence of magnetic field stimulation on the growth and biochemical parameters in Phaseolus vulgaris L. Journal of Microbiology, Biotechnology and Food Sciences, 2021, 548-551.

Müftügil, N. (1985). The peroxidase enzyme activity of some vegetables and its resistance to heat. Journal of the Science of Food and Agriculture, 36(9), 877-880.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15(3), 473-497.

Naz, H. (2011). Nigella sativa: the miraculous herb. Pak. J. Biochem. Mol. Biol, 44(1), 44-48.

OBAID, A. (2012). Raja'a Mohammed Obaid AL-Temimi (Doctoral dissertation, Council of the College of Education for Pure Sciences, University of Diyala).

of magnetic fields on germination, early growth characteristics and activities of some enzymes in Nigella sativa L. seeds.

POURAKBAR, L., ASADI, S. M., & ASHRAFI, R. (2012). Effect

Radhakrishnan, R. (2019). Magnetic field regulates plant functions, growth and enhances tolerance against environmental

Reina, F. G., & Pascual, L. A. (2001). Influence of a stationary magnetic field on water relations in lettuce seeds. Part I: Theoretical considerations. Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association, 22(8), 589-595.

S.H. Chapter 13—Cumin. In Medicinal Plants of South Asia; Hanif,M.A., Nawaz, H., Khan, M.M., Byrne, H.J., Eds.; Elsevier: Amsterdam, The Netherlands, 2020; pp. 165–178.

Sahebjamei, H., Abdolmaleki, P., & Ghanati, F. (2007). Effects of magnetic field on the antioxidant enzyme activities of suspension‐ cultured tobacco cells. Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association, 28(1), 42-47.

Shane, S. J., Riley, C., Laurie, J. H., & Boutilier, M. (1951). Thiosemicarbazones in Tuberculosis. Canadian Medical Association Journal, 64(4), 289.

Sogut, S., Hanci, V., ... & Caskurlu, T. U. R. H. A. N. (2017). Comparison of combined antioxidants and thymoquinone in the prevention of testis ischemia–reperfusion injury. Andrology, 5(1), 119-124.

stresses. Physiology and Molecular Biology of Plants, 25(5), 1107- 1119.

Sultan, M. T., Butt, M. S., Ahmad, R. S., Pasha, I., Ahmad, A. N., & Qayyum , M. M. N. (2012). Supplementation of Nigella sativa fixed and essential oil mediates potassium bromate induced oxidative stress and multiple organ toxicity. Pakistan journal of pharmaceutical sciences, 25(1).

Tiji, S., Bouhrim, M., Addi, M., Drouet, S., Lorenzo, J. M., Hano, C., & Mimouni, M. (2021). Linking the phytochemicals and the α- glucosidase and α-amylase enzyme inhibitory effects of Nigella sativa seed extracts. Foods, 10(8), 1818.

Towill, L. E., & Mazur, P. (1975). Studies on the reduction of 2, 3, 5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures. Canadian Journal of Botany, 53(11), 1097-1102.

Trigiano, R. N., & Gray, D. J. (2016). Plant tissue culture, development, and biotechnology. CRC Press..

Ulgen, C., Yildirim, A. B., Sahin, G., & Turker, A. U. (2021). Do magnetic field applications affect in vitro regeneration, growth, phenolic profiles, antioxidant potential and defense enzyme activities (SOD, CAT and PAL) in lemon balm (Melissa officinalis L.). Industrial Crops and Products, 169, 113624.