Studies on Cytotoxic and Genotoxic potential of Ethanolic extract of Lawsonia inermis leaves

https://doi.org/10.53730/ijhs.v6nS1.5842

Authors

  • Mohammad Munawar T Department of Biological and Chemical Engineering, Mekelle Institute of Technology (MIT), Mekelle University, Mekelle-1632, Ethiopia
  • Chinta Koteswara Rao Data Processing officer, A.P.State Biodiversity Board, Guntur-522508, Andhra Pradesh, India
  • Andanamala Vijay Rajesh Dean (Administration) and Professor, S&H Department, Vikas Group of Institution, Vijayawada-521212, Andhra Pradesh, India
  • Udit Narayan Sharma B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut- 250005, Uttar Pradesh, India
  • Istuti Gupta B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut- 250005, Uttar Pradesh, India
  • Surya Prakash D.V. Assistant Professor, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut- 250005, Uttar Pradesh, India

Keywords:

Lawsonia inermis, Cytotoxicity, Genotoxicity, Miotic index, Blood sample

Abstract

Lawsonia inermis is widely known as henna and belongs to Lythraceae family. It contains various phytochemica constituents in every part of plant and shows various pharmacological properties. Secondary metabolites are acts as antioxidants and these are very high in this plant species. In this research studies, ethanolic extract of Lawsonia inermis was performed in the blood sample culture for cytotoxicity and genotoxicity effects. The both of the effects of plant extracts were evaluated through measurement of mitotic index (MI), sister chromatid exchanges (SCE) and cell proliferation kinetics (CPK).  The blood sample culture was treated with control and various concentrations of extracts (500μg/ml and 1000μg/ml). The mean of MI, SCE and CPK for control were found to be 4.51,  5.19 and 0.52 respectively where as for 500μg/ml of Lawsonia inermis extracts are 4.33, 4.87 and 0.50 respectively and for 1000 μg/ml extracts are 4.18, 4.68 and 0.48 respectively. From the results, we can conclude that the MI and frequency of SCES of ethanolic plant extracts are remarkably similar to that of control which indicates that the Lawsonia inermis plant extracts have no substantial cytotoxicity and genotoxicity effects in cultured blood lymphocytes.

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Published

11-04-2022

How to Cite

Munawar T, M., Rao, C. K., Rajesh, A. V., Sharma, U. N., Gupta, I., & Surya Prakash, D. V. (2022). Studies on Cytotoxic and Genotoxic potential of Ethanolic extract of Lawsonia inermis leaves. International Journal of Health Sciences, 6(S1), 4271–4279. https://doi.org/10.53730/ijhs.v6nS1.5842

Issue

Vol. 6 No. S1 (2022)

Section

Peer Review Articles
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