Histological and chronological study of myostatin (MSTN) role on muscles growth in domestic broiler chicken

https://doi.org/10.53730/ijhs.v6nS3.6053

Authors

  • Esraa Abdul Khaliq Zegyer Department of Pathological Analyses, College of Science, Wasit University, Wasit- Iraq
  • Ali Mahdi Mutlag Department of Pathological Analyses, College of Science, Wasit University, Wasit- Iraq

Keywords:

skeletal muscle, myostatin (MSTN), immunohistochemistry, broiler chicken

Abstract

Chicken meat have a low-cholesterol levels, low-fat with a high unsaturation amount of fatty acids, which moreover be considered as functional foods, which provide bioactive ingredients with advantageous effects on human health like vitamins and antioxidants. Therefore, this project was designed to focusing on the relationship between increased the growth curve of skeletal muscles and the role of Myostatin (MSTN) in local domestic broiler chicken. The 50 birds of male domestic broiler chicken were collected from commercial field of Al -Kut, and according ages, the birds were divided into five groups (1day, 8 day, 15 day, 22day, and 30day). The samples of skeletal muscles were taken from the breast and leg areas. All samples were exposed to histological investigation by H&E stains, as well as immunohistochemical technique by a primary and secondary antibody of MSTN through paraffin-embedded sections. Histological results apparent a general structure of skeletal muscles at different ages through synchronous growth development of chicken.  Immunohistochemical investigation appeared the different attribution of MSTN reactive in the skeletal muscle sections at sequence ages of chicken. Surface blots analysis of MSTN in skeletal muscle at the five experimental stages was (3.096, 10.049, 12.927, 18.453, 22.744) respectively. 

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Published

15-04-2022

How to Cite

Zegyer, E. A. K., & Mutlag, A. M. (2022). Histological and chronological study of myostatin (MSTN) role on muscles growth in domestic broiler chicken. International Journal of Health Sciences, 6(S3), 2415–2432. https://doi.org/10.53730/ijhs.v6nS3.6053

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Section

Peer Review Articles