Histopathological and reproductive effect of tamsulosin and finasteride on induced Benign prostate hyperplasia in mice

Shukur Mahmood Yaseen; Firas R. Al-Samarai; Huda F. Hasan

doi:10.53730/ijhs.v6nS1.6062

Authors

Shukur Mahmood Yaseen Medical Biology and Anatomy Department, Faculty of Medicine, Diyala University, Iraq
Firas R. Al-Samarai Department of Veterinary Public Health, College of Veterinary Medicine, University of Baghdad, Iraq
Huda F. Hasan Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Baghdad, Iraq

Keywords:

BPH, tamsulosin, finasteride, their Combination, Histopathological Changes, testosterone, Sperm Count %

Abstract

The prostate hyperplasia (BPH) was induced in male mice by subcutaneous injection of testosterone propionate (20 mg/kg) for 30 days. Fifty mice were divided equally into five groups, the first group (G1) was (negative control group), and the second group (G2) was (positive Control group) induced BPH previously and inoculated orally distilled water. The third group (G3) was induced BPH and treated orally with tamsulosin (0.156mg /kg), the fourth group (G4) was induced BPH and treated orally with Finasteride (1.495 mg /kg) and finally, the fifth (G5) was induced BPH and treated orally with a combination of tamsulosin and finasteride (0.0825, 0.7475) mg/kg respectively. The histopathological results showed prostate hyperplasia and abnormal proliferation of the epithelial and stromal cells with inflammation cells accompanying stenosis of acini in G2. Whereas showed a decrease in hyperplasia, and epithelial layer thickness in G3 and G4. Furthermore, prostate shrinkage and the glandular cavities trended towards the normal approach of the prostate in G5. The testosterone levels in G2 showed a significant increase (P<0.05) as compared to other treated groups with a more significant decrease in G4 compared to other treated groups.

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References

Atawia, Reem T.; Mariane G. Tadros; Amani E. Khalif; Hisham A. Mosli, and Ashraf B. Abdel-Naim. (2013). “Role of the Phytoestrogenic, pro-Apoptotic and Anti-Oxidative Properties of Silymarin in Inhibiting Experimental Benign Prostatic Hyperplasia in Rats.” Toxicology Letters 219 (2): 160–69. https://doi.org/10.1016/j.toxlet.2013.03.002.

Askar, S. J. (2017). Role of aqueous extract of Crocus sativus to improvement of sexual dysfunctions induced by imipramine in male mice. The Iraqi Journal of Veterinary Medicine, 41(1), 92–97. https://doi.org/10.30539/iraqijvm.v41i1.88.

Angrimani, Daniel S.R.; Luana C. Bicudo; Nuria Llamas Luceño; Bruno R. Rui; Matheus F. Silva; João D.A. Losano; Bart Leeman’s; Ann Van Soom; and Camila I. Vannucchi (2020). Does Finasteride Treatment for Benign Prostatic Hyperplasia Influence Sperm DNA Integrity in Dogs? Basic and Clinical Andrology 30 (1): 1–8. https://doi.org/10.1186/s12610-020-00108-2.

Benjamin O Odusanya ; Kehinde H Tijani; Emmanuel A Jeje ; Moses A Ogunjimi ; and Rufus W Ojewola (2017). Short-term Effect of Tamsulosin and Finasteride Monotherapy and their Combination on Nigerian Men with Benign Prostatic Hyperplasia. Niger J Surg. Jan-Jun; 23(1): 5–10.

Carson, Culley, and Roger Rittmaster. (2003). “The Role of Dihydrotestosterone in Benign Prostatic Hyperplasia.” Urology. Elsevier Inc. https://doi.org/10.1016/S0090-4295(03)00045-1.

Chiba, Koji; Kohei Yamaguchi; Fuping Li; Makoto Ando; and Masato Fujisawa. (2011). “Finasteride-Associated Male Infertility.” Fertility and Sterility 95 (5): 1786.e9-1786.e11. https://doi.org/10.1016/j.fertnstert.2010.12.001.

Cunha de Medeiros, P; Samelo, R. R ; Silva, A. P. G ; da Silva Araujo Santiago, M ; Duarte, F. A; de Castro, Í. B; and Perobelli, J. E. (2019). Prepubertal exposure to low doses of sodium arsenite impairs spermatogenesis and epididymal histophysiology in rats. Environmental Toxicology, 34(1), 83–91.

Dahm, Philipp; Michelle Brasure; Roderick MacDonald; Carin M. Olson; Victoria A. Nelson; Howard A. Fink; Bruce Rwabasonga; Michael C. Risk; and Timothy J. Wilt. (2017). Comparative Effectiveness of Newer Medications for Lower Urinary Tract Symptoms Attributed to Benign Prostatic Hyperplasia: A Systematic Review and Meta-Analysis. European Urology 71 (4). https://doi.org/10.1016/j.eururo.2016.09.032.

Daud, Dzulsuhaimi; Nur Faza Mohd Fekery; and Nooraain Hashim. (2017). Reproductive Health of Rats with Benign Prostatic Hyperplasia Following Cosmos Caudatus Ethanolic Extract Consumption. Journal of Applied Pharmaceutical Science 7 (6): 202–5. https://doi.org/10.7324/JAPS.2017.70630.

Fano, Diego ; Cinthya Vásquez-Velásquez ; Cynthia Gonzales-Castañeda ; Emanuel Guajardo-Correa ; Pedro A. Orihuela ; and Gustavo F. Gonzales. (2017). “N-Butanol and Aqueous Fractions of Red Maca Methanolic Extract Exerts Opposite Effects on Androgen and Oestrogens Receptors (Alpha and Beta) in Rats with Testosterone-Induced Benign Prostatic Hyperplasia.” Evidence-Based Complementary and Alternative Medicine 2017. https://doi.org/10.1155/2017/9124240.

Flores, RB; DSR Angrimani; BR Rui; MM Brito; RA Abreu; and CI Vannucchi. (2017). “The Influence of Benign Prostatic Hyperplasia on Sperm Morphological Features and Sperm DNA Integrity in Dogs.” Reproduction in Domestic Animals 52 (April). https://doi.org/10.1111/rda.12817.

Gravas, Stavros; and Matthias Oelke. (2010). “Current Status of 5α-Reductase Inhibitors in the Management of Lower Urinary Tract Symptoms and BPH.” World Journal of Urology 28 (1): 9–15. https://doi.org/10.1007/s00345-009-0493-y

Hollingsworth, John M; and Timothy J. Wilt. (2014). “Lower Urinary Tract Symptoms in Men.” BMJ (Online). BMJ Publishing Group. https://doi.org/10.1136/bmj.g4474.

Hyun Hwangbo; Da He Kwon; Eun Ok Choi ; Min Yeong Kim ; Kyu Im Ahn ; Seon Yeong Ji1 ; Jong Sik Kim ; Kyung-Il Kim ; No-Jin Park ; Bum Hoi Kim ; Gi-Young Kim ; Su-Hyun Hong ; Cheol Park ; Ji-Suk Jeong ; and Yung Hyun Choi1. (2018). Corni Fructus attenuates testosterone-induced benign prostatic hyperplasia by suppressing 5α-reductase and androgen receptor expression in rats. Nutrition Research and Practice 2018;12(5):378-386 ⓒ2018 The Korean Nutrition Society and the Korean Society of Community Nutrition.

Ishizuka, Osamu; Osamu Nishizawa; Yoshihiko Hirao; and Shinichi Ohshima. (2002). “Evidence-Based Meta-Analysis of Pharmacotherapy for Benign Prostatic Hypertrophy.” International Journal of Urology 9 (11): 607–12. https://doi.org/10.1046/j.1442-2042.2002.00539.x.

Mbaka, Godwin; Charles Anunobi; Samson Ogunsina; and Daniel Osiagwu. (2017). “Histomorphological Changes in Induced Benign Prostatic Hyperplasia with Exogenous Testosterone and Estradiol in Adult Male Rats Treated with Aqueous Ethanol Extract of Secamone Afzelii.” Egyptian Journal of Basic and Applied Sciences 4 (1): 15–21. https://doi.org/10.1016/j.ejbas.2016.11.003.

Mbaka, Godwin; Steve Ogbonnia; Adeola Sulaiman; and Daniel Osiagwu. (2019). “Histomorphological Effects of the Oil Extract of Sphenocentrum Jollyanum Seed on Benign Prostatic Hyperplasia Induced by Exogenous Testosterone and Estradiol in Adult Wistar Rats.” Avicenna Journal of Phytomedicine 9 (1): 21–33. https://doi.org/10.22038/ajp.2018.24039.1882.

Mokhtari, M.; M. Shariati; and J. Amiri. (2007). “Effects of Tamsolusin on Serum Testosterone and Gonadotropins Concentration in Adult Male Rats.” Journal of Rafsanjan University of Medical Sciences 6 (1): 1–6. http://journal.rums.ac.ir/article-1-282-en.html.

Roehrborn, Claus G.; and Raymond C. Rosen. (2008). “Medical Therapy Options for Aging Men with Benign Prostatic Hyperplasia: Focus on Alfuzosin 10 Mg Once Daily.” Clinical Interventions in Aging. Dove Press. https://doi.org/10.2147/cia.s3635.

Silverberg, Kaylen M.; and Tom Turner. 2012. “Evaluation of Sperm.” In Textbook of Assisted Reproductive Techniques, Volume One: Laboratory Perspectives. Informa Healthcare. https://doi.org/10.3109/9781841849713.004.

Strand, Douglas W.; Daniel N. Costa ; Franto Francis; William A. Ricke ; and Claus G. Roehrborn. (2017). “Targeting Phenotypic Heterogeneity in Benign Prostatic Hyperplasia.” Differentiation. Elsevier Ltd. https://doi.org/10.1016/j.diff.2017.07.005.

Traish, Abdulmaged M.; Karim Sultan Haider; Gheorghe Doros; and Ahmad Haider (2015). Finasteride, Not Tamsulosin, Increases Severity of Erectile Dysfunction and Decreases Testosterone Levels in Men with Benign Prostatic Hyperplasia. Hormone Molecular Biology and Clinical Investigation 23 (3). https://doi.org/10.1515/hmbci-2015-0015.

Wang, Xiaolong; Yiming Wang ; Christian Gratzke ; Christian Sterr ; Qingfeng Yu ; Bingsheng Li ; Frank Strittmatter ; et al. (2019). “Ghrelin Aggravates Prostate Enlargement in Rats with Testosterone-Induced Benign Prostatic Hyperplasia, Stromal Cell Proliferation, and Smooth Muscle Contraction in Human Prostate Tissues.” Oxidative Medicine and Cellular Longevity 2019 (November). https://doi.org/10.1155/2019/4748312.

Yegane Kohestani; Bentolhoda Kohestani; Zahra Shirmohamadi; and Masoumeh Faghani, (2020): Effect of tamsulosin on testis histopathology and serum hormones in adult rats: Experimental study. Int J Reprod Biomed. 2020 Jul; 18(7): 531–538.. doi: 10.18502/ijrm.v13i7.7370

Zhou, Zhongbao; Yuanshan Cui; Jitao Wu; Rui Ding; Tong Cai; and Zhenli Gao. (2019). “Meta-Analysis of the Efficacy and Safety of Combination of Tamsulosin plus Dutasteride Compared with Tamsulosin Monotherapy in Treating Benign Prostatic Hyperplasia.” BMC Urology 19 (1). https://doi.org/10.1186/s12894-019-0446-8.