Genotypic assay to determine some virulence factors of Uropathogenic E. coli (UPEC) isolates

Tsahel H. Al-Dulaimi; Ilham A Bunyan; Thikra A. Banimuslem

doi:10.53730/ijhs.v6nS4.6289

Authors

Tsahel H. Al-Dulaimi Student at University of Babylon, College of Medicine, Iraq
Ilham A Bunyan
Ilhamalsaedi2oo8@gmail.com
Dept. of Microbiology, College of Medicine, University of Babylon, Iraq
Thikra A. Banimuslem Al-Qasim Green University, College of Science, Babylon, Iraq

Keywords:

Gene, PCR, E. coli, Urinary tract infections, Hemolysin

Abstract

A total of 179 urine samples were collected from patients suffering from urinary tract infections were admitted and visit Al-Hilla General Teaching Hospital in Al-Hilla City, during a period from April 2021 to December 2021, from both sex (male and female). Out of 179,123 (68.7%) were positive culture, whereas 56 (31.3%) samples showed no bacterial growth, To confirm the identification of E. coli by use selective media (EMB agar medium, biochemical tests, automated Vitek 2 system and 16s RNA specific primer by the presence of (1492 bp) compared with allelic ladder, it was found that, E. coli were deliberated the main an etiological causes UTI to other types bacteria which constitute 56/123 (45.5%), [45/56 (80.4%) from female and 11/56 (19.6%) from male], while 67/123 (54.4%) were related to other types of bacteria. Molecular detection of some virulence factors genes were studied, out of 56 E. coli isolates, hlyA gene was detected in 21/56 (37.5%) isolates by the presence of (1177 bp) and sat gene was detected in 35/56 (62.5%) isolates by the presence of (410 bp) compared with allelic ladder.

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