Evaluation of in-vitro anti-cancer activity of aqueous extract of the C. maxima seed
Keywords:
aqueous extract, C. maxima seed, MCF-7, DPPH assay, anti-cancer activityAbstract
The most frequent cancer in women worldwide is breast cancer, it is also the second leading cause of cancer-related death in women. As a consequence, there is a pressing need to develop more effective cancer medicines. The seed of pumpkin C. maxima reported to have medicinal properties and pharmacological activities like anti-inflammatory, antioxidant and anti-microbial activity. In the present study, we performed DPPH free radical scavenging activity and anti-microbial activity against microorganisms E. coli, P. aeruginosa, A. bracilences, S. aureus using aqueous extract of C. maxima seed were preferred. MCF-7 breast cancer cells were used to test anti-cancer activities In -vitro. The outcomes revealed high anti-oxidant activity of the extract 0.781 ± 0.016 in 100 μg/ml and anti-microbial activity showed the most prominent activity of the extract against all the tested microorganisms. The MTT assay showed IC50 value of 45.40 μg/ml of the extract. Further, the Ao/EtBr staining proved that the methanolic extract of C. maxima possess protective activity against MCF-7 cell lines.
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