In vitro antioxidant and antibacterial activity of Quercetin isolated from Indigofera aspalathoides and Quercetin-Zinc metal complex

Shalini Elumalai; Sangeetha Soundararajan; Petchiammal Kanagaraj; Deepa Rani Sadhasivam

doi:10.53730/ijhs.v6nS3.6859

Authors

Shalini Elumalai PG & Research Department of Zoology, Pachaiyappa’s College, Chennai, Tamil Nadu, India
Sangeetha Soundararajan PG & Research Department of Zoology, Pachaiyappa’s College, Chennai, Tamil Nadu, India
Petchiammal Kanagaraj PG & Research Department of Zoology, Pachaiyappa’s College, Chennai, Tamil Nadu, India
Deepa Rani Sadhasivam PG & Research Department of Zoology, Ethiraj College for Women, Chennai, Tamil Nadu, India

Keywords:

indigofera aspalathoides, quercetin, zinc metal complex, antioxidant, antibacterial

Abstract

Plant secondary metabolites play a crucial role in the therapeutic applications due to their non toxic nature. The present study was aimed to explore the antioxidant and antibacterial potential of a natural flavonoid Quercetin isolated from the plant Indigofera aspalathoides and Quercetin-Zinc (Qn-Zn) metal complex. The study results explored that, the both Quercetin and Qn-Zn metal complex displayed a strong free radicals quencher against the DPPH, ABTS and superoxide anion free radicals. In addition to that, it has also possessed antibacterial activity against the gram positive (Staphylococcus aureus, Bacillus Subtilis, Enterococcus faecalis) and gram negative (Escherichia coli, Pseudomonas aeruginosa) human pathogenic bacteria. Moreover, we found that and Qn-Zn metal complex displayed slightly better activity than the Quercetin. Overall, the present findings are demonstrated that, the biological activities of natural flavonoid Quercetin were increased, when it’s combined with Zinc metal complex.

Downloads

Download data is not yet available.

References

Al-Mamun, M., Yamaki, K., Masumizu, T., Nakai, Y., Saito, K., Sano, H. and Tamura, Y., 2007. Superoxide anion radical scavenging activities of herbs and pastures in northern Japan determined using electron spin resonance spectrometry. International journal of biological sciences, 3(6), p.349.

Alonso-Castro, A.J., Domínguez, F., Maldonado-Miranda, J.J., Castillo-Pérez, L.J., Carranza-Álvarez, C., Solano, E., Isiordia-Espinoza, M.A., del Carmen Juárez-Vázquez, M., Zapata-Morales, J.R., Argueta-Fuertes, M.A. and Ruiz-Padilla, A.J., 2017. Use of medicinal plants by health professionals in Mexico. Journal of Ethnopharmacology, 198, pp.81-86.

Arnao, M.B., Cano, A. and Acosta, M., 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food chemistry, 73(2), pp.239-244.

Bathaie, S.Z. and Tamanoi, F., 2015. Mechanism of the anticancer effect of phytochemicals. Academic Press.

Bennetts, H.W., Underwood, E.J. and Shier, F.L., 1946. A specific breeding problem of sheep on subterranean clover pastures in Western Australia. Veterinary Journal, 102, pp.348-352.

Bhuvaneswari, R.A. and Balasundaram, C., 2006. Traditional Indian herbal extracts used in vitro against growth of the pathogenic bacteria–Aeromonashydrophila. Israeli Journal of Aquaculture-Bamidgeh, 58, p.20436.

Blois, M.S., 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), pp.1199-1200.

Bone, K., Simon Mills, M.C.P.P. and Fnimh, M.A., 2012. Principles and practice of phytotherapy: modern herbal medicine. Elsevier Health Sciences.

Bratu, M.M., Birghila, S.E.M.A.G.H.I.U.L., Miresan, H., Negreanu-Pirjol, T., Prajitura, C. and Calinescu, M., 2014. Biological activities of Zn (II) and Cu (II) complexes with quercetin and rutin: Antioxidant properties and UV-protection capacity. Rev. Chim.(Bucharest), 65, pp.544-549.

Costello, L.C. and Franklin, R.B., 2012. Cytotoxic/tumor suppressor role of zinc for the treatment of cancer: an enigma and an opportunity. Expert review of anticancer therapy, 12(1), pp.121-128.

Eisner, T., 1990. Chemical prospecting. A call for action. Ecology, Economic and Ethics: The Broken Circles. Yale University Press, New Haven, CT.

Ferraz, C.R., Franciosi, A., Emidio, N.B., Rasquel-Oliveira, F.S., Manchope, M.F., Carvalho, T.T., Artero, N.A., Fattori, V., Vicentini, F.T., Casagrande, R. and Verri Jr, W.A., 2021. Quercetin as an antiinflammatory analgesic. In A Centum of Valuable Plant Bioactives (pp. 319-347). Academic Press.

Goławska, S., Sprawka, I., Łukasik, I. and Goławski, A., 2014. Are naringenin and quercetin useful chemicals in pest-management strategies?. Journal of pest science, 87(1), pp.173-180.

Hussein, R.A. and El-Anssary, A.A., 2019. Plants secondary metabolites: the key drivers of the pharmacological actions of medicinal plants. Herbal medicine, 1, p.13.

Ikeda, N.E.A., Novak, E.M., Maria, D.A., Velosa, A.S. and Pereira, R.M.S., 2015. Synthesis, characterization and biological evaluation of Rutin–zinc (II) flavonoid-metal complex. Chemico-biological interactions, 239, pp.184-191.

Jain, C., Khatana, S. and Vijayvergia, R., 2019. Bioactivity of secondary metabolites of various plants: a review. Int. J. Pharm. Sci. Res, 10(2), pp.494-504.

Khanam, Z., Wen, C.S. and Bhat, I.U.H., 2015. Phytochemical screening and antimicrobial activity of root and stem extracts of wild Eurycoma longifolia Jack (Tongkat Ali). Journal of King Saud University-Science, 27(1), pp.23-30.

Long, L.H., Kwee, D.C.T. and Halliwell, B., 2000. The antioxidant activities of seasonings used in Asian cooking. Powerful antioxidant activity of dark soy sauce revealed using the ABTS assay. Free radical research, 32(2), pp.181-186.

Maizura, M., Aminah, A. and Wan Aida, W.M., 2011. Total phenolic content and antioxidant activity of kesum (Polygonum minus), ginger (Zingiber officinale) and turmeric (Curcuma longa) extract. International Food Research Journal, 18(2).

Ozturk, S. and Ercisli, S., 2006. Chemical composition and in vitro antibacterial activity of Seseli libanotis. World Journal of Microbiology and Biotechnology, 22(3), pp.261-265.

Philips, A., Philip, S., Arul, V., Padmakeerthiga, B., Renju, V., Santha, S. and Sethupathy, S., 2010. Free radical scavenging activity of leaf extracts of Indigofera aspalathoides-An in vitro analysis. J Pharm Sci Res, 2(6), pp.322-328.

Pietta, P.G., 2000. Flavonoids as antioxidants. Journal of natural products, 63(7), pp.1035-1042.

Rajkapoor, B., Jayakar, B. and Murugesh, N., 2004. Antitumor activity of Indigofera aspalathoides on Ehrlich ascites carcinoma in mice. Indian journal of Pharmacology, 36(1), p.38.

Raju, Y.R., Yugandhar, P. and Savithramma, N., 2014. Documentation of ethnomedicinal knowledge of hilly tract areas of east Godavri district of Andhra Pradesh, India. Int J Pharm Pharm Sci, 6(4), pp.369-374.

Ramya, V., Madhu-Bala, V., Prakash-Shyam, K., Gowdhami, B., Sathiya-Priya, K., Vignesh, K., Vani, B. and Kadalmani, B., 2021. Cytotoxic activity of Indigofera aspalathoides (Vahl.) extracts in cervical cancer (HeLa) cells: Ascorbic acid adjuvant treatment enhances the activity. Phytomedicine Plus, 1(4), p.100142.

Robak, J. and Gryglewski, R.J., 1988. Flavonoids are scavengers of superoxide anions. Biochemical pharmacology, 37(5), pp.837-841.

Rodriguez-Arce, E. and Saldias, M., 2021. Antioxidant properties of flavonoid metal complexes and their potential inclusion in the development of novel strategies for the treatment against neurodegenerative diseases. Biomedicine & Pharmacotherapy, 143, p.112236.

Saeed, N., Khan, M.R. and Shabbir, M., 2012. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC complementary and alternative medicine, 12(1), pp.1-12.

Safari, M. and Ahmady-Asbchin, S., 2019. Evaluation of antioxidant and antibacterial activities of methanolic extract of medlar (Mespilus germanica L.) leaves. Biotechnology & biotechnological equipment, 33(1), pp.372-378.

Shiva, M.P., 1998. Inventory of Forest Resources for Sustainable Management & Biodiversity Conservation with Lists of Multipurpose Tree Species Yielding Both Timber & Non-timber Forest Products (NTFPs), and Shrub & Herb Species of NTFP Importance. Indus Publishing.

Sivagnanam, S.K., Rao, M.R.K. and Balasubramanian, M.P., 2012. Chemotherapeutic efficacy of Indigofera aspalathoides on 20-methylcholanthrene-induced fibrosarcoma in rats. International Scholarly Research Notices, 2012.

Skrovankova, S., Mišurcová, L. and Machů, L., 2012. Antioxidant activity and protecting health effects of common medicinal plants. Advances in food and nutrition research, 67, pp.75-139.

Suhaj, M., 2006. Spice antioxidants isolation and their antiradical activity: a review. Journal of food composition and analysis, 19(6-7), pp.531-537.

Swarnalatha S, Umamaheswari A, Puratchikody, A. Immunomodulatory activity of kaempferol 5-O-β-d-glucopyranoside from Indigofera aspalathoides Vahl ex DC.(Papilionaceae). 2015. Medicinal Chemistry Research, 24(7), pp.2889-2897.

Tamilselvi, N., Dhamotharan, R., Krishnamoorthy, P., Arumugam, P. and Sagathevan, E., 2011. Antifungal Activity of Indigofera aspalathoides (Shivanar Vembu) Vahl ex Dc. Drug Invention Today, 3(11).

Uskokovic-Markovic, S., Milenković, M. and Pavun, L., 2020. Zinc-quercetin complex: From determination to bioactivity. Acta agriculturae Serbica, 25(50), pp.113-120.

Wiegand, I., Hilpert, K. and Hancock, R.E., 2008. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols, 3(2), pp.163-175.

Wilson, E.O. and Peter, F.M., 1988. Screening plants for new medicines. In Biodiversity. National Academies Press (US).

World Health Organization, 2019. WHO global report on traditional and complementary medicine 2019. World Health Organization.

Xie, Y., Yang, W., Tang, F., Chen, X. and Ren, L., 2015. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), pp.132-149.

Yirga, G., Teferi, M. and Kasaye, M., 2011. Survey of medicinal plants used to treat human ailments in Hawzen district, Northern Ethiopia. International Journal of Biodiversity and Conservation, 3(13), pp.709-714.