Nitrofurantoin: Furious against bacteria? An in vitro study to test nitrofurantoin as an intracanal medicament against Enterococcus Faecalis

Navjot Singh Mann; Ashu Jhamb; Kunal Sharma; Manu Rana; Divya Batra

doi:10.53730/ijhs.v6nS2.7188

Authors

Navjot Singh Mann Professor and Department Head, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital Gulabgarh, Derabassi
Ashu Jhamb Professor, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital Gulabgarh, Derabassi
Kunal Sharma Student in Postgraduate Studies, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital Gulabgarh, Derabassi
Manu Rana Reader, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital Gulabgarh, Derabassi
Divya Batra Reader, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital Gulabgarh, Derabassi

Keywords:

nitrofurantoin, MTAP, enterococcus faecalis, calcium hydroxide

Abstract

In vitro evaluation of nitrofurantoin as an intracanal medicament against Enterococcus Faecalis. Materials and Methods: 150 single-rooted premolar teeth were chosen and instrumented using a Protaper gold rotary file system; the root canals of these teeth were inoculated for 21 days with an Enterococcus faecalis bacterial solution. The canals were inoculated with Nitrofurantoin, Calcium hydroxide, and MTAP after 21 days and left for 7 days. On the eighth day, the samples were examined to determine the degree of infection. The study's findings revealed that both Nitrofurantoin and MTAP were very successful, with no Colony Forming Units (CFU) production in their respective groups. The current investigation indicated that nitrofurantoin is effective in eradicating E. faecalis cells in pure culture as well as root canals.

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References

Siqueira JF Jr., Rocas IN. Analysis of microorganisms linked with failure endodontic therapy using polymerase chain reaction. Oral Surgery Oral Medicine Oral Pathology Oral radiology and endoscopy, 2004;97:85-94.

C. Sedgley, G. Buck, and O. Appelbe, "Prevalence of Enterococcus faecalis in endodontic patients utilising culture and PCR," Journal of Endodontia, vol. 32, no. 2, pp. 104–109, 2006.

Microorganisms in root canal-treated teeth from a German population, I. N. Rôças, M. Hulsmann, and J. F. Siqueira Jr., Journal of Endodontia, vol. 34, no. 8, pp. 926–931, 2008.

Q. Wang, C. F. Zhang, C. H. Chu, and X. F. Zhu, "Prevalence of Enterococcus faecalis in saliva and filled root canals of teeth associated with apical periodontitis," International Journal of Dental Research, vol. Journal of Oral Science, vol. 4, no. 1, 2012, pp. 19–23.

Hancock, H. H. III, A. Sigurdsson, M. Trope, and J. Moiseiwitsch, "Bacteria isolated following unsatisfactory endodontic treatment in a North American population," Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 91, no. 5, pp. 579–586, 2001.

Biofilms: survival mechanisms of clinically important bacteria, R. M. Donlan and J. W. Costerton, Clinical Microbiology Reviews, vol. 15, no. 2, pp. 167–193, 2002.

Endodontic Topics, vol. 10, no. 1, pp. 30–76, 2005. M. Hülsmann, O. A. Peters, and P. M. H. Dummer, "Mechanical preparation of root canals: setting aims, techniques, and means."

P. V. Abbott, W. R. Hume, and J. W. Pearman, "Antibiotics and Endodontics," Australian Dental Journal, vol. 35, no. 1, 1990, pp. 50–60.

J. J. Segura-Egea, K. Gould, B. H. en, and colleagues, "Antibiotics in Endodontics: A Review," International Endodontic Journal, vol. 50, no. 12, pp. 1169–1184, 2017.

10th. American Dental Association Council on Scientific Affairs, "Antibiotic Use in Dentistry," Journal of the American Dental Association, vol. 128, no. 5, p. 648, 1997.

A. Parhizkar, H. Nojehdehian, and S. Asgary, "Triple antibiotic paste: significant roles and uses in endodontics: a review," Restorative Dentistry & Endodontics, vol. 43, no. 3, 2003.2018 item e28

A. Karczewski, S. A. Feitosa, E. I. Hamer, and colleagues, "Clindamycin modified triple antibiotic nanofibers: a stain-free antimicrobial intracanal drug delivery system," Journal of Endodontia, vol.155–162, vol. 44, no. 1, 2018.

B. A. Cunha, Obstetrical & Gynecological Survey, vol. 44, no. 5, pp. 399–406, 1989.

"Nitrofurantoin is active against vancomycin-resistant enterococci," Antimicrobial Agents and Chemotherapy, vol. 45, no. 1, pp. 324–326, 2001.

Biofilms: survival mechanisms of clinically important bacteria, R. M. Donlan and J. W. Costerton, Clinical Microbiology Reviews, vol. 15, no. 2, pp. 167–193, 2002.

S. M. Ozbek, A. Ozbek, and A. S. Erdorgan, "Real-time PCR SYBR green analysis of Enterococcus faecalis in samples from Turkish patients with primary endodontic infections and failed endodontic therapy," Journal of Applied Oral Science, vol. 17, no. 5, pp. 370–374, 2009.

Love RM. Endodontic failure caused by Enterococcus faecalis: a mechanism. International Endod J 2001; 34:399-405.

Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms of Enterococcus faecalis resistance to calcium hydroxide. International Endod J 2002;35:221-8.

Nerwich, D. Figdor, D. Endo, and H. Messer. The pH of root dentin changed over a 4-week period after root canal dressing with calcium hydroxide. Endocrinology 1993;96:302-6.

Minana M, Carnes D, Walker WI. pH alterations at the surface of root dentin following intracanal calcium hydroxide dressing. Endocrinology 2001;27:43-5.

S. S. Long, C. G. Prober, and M. Fischer, "Principles and Practice of Pediatric Infectious Diseases," 292-Antimicrobial Agents, 5th edition, 2018, pp. 1499–1531.e3.

P. M. Fitzpatrick and C. C. McOsker, "Nitrofurantoin: Mechanism of Action and Implications for Resistance Development in Common Uropathogens," Journal of Antimicrobial Chemotherapy, vol. 33, Supplement A, pp. 23–30, 1994.

A. A. Algarni, G. H. Yassen, and R. L. Gregory, "Inhibitory effect of antibiotic-loaded gels on biofilm formation by Enterococcus faecalis and Porphyromonas gingivalis," Journal of Oral Science, vol. 57, no. 3, pp. 213–218, 2015.

M. A. Mozayeni, A. Haeri, O. Dianat, and A. R. Jafari, "In vitro research of the antimicrobial effects of four intracanal medicaments on Enterococcus faecalis," Iranian Endodontic Journal, vol. 9, no. 3, pp. 195–198, 2014.

K. Ravi, "Antimicrobial Efficacy of Different Intracanal Medication Against Enterococcus Faecalis," Journal of Pharmaceutical Sciences and Research, vol. 9, no. 10, pp. 1861–1863, 2017.

J. Sabarathinam and N. P. Muralidharan, "In vitro evaluation of the antimicrobial efficiency of four different intracanal medicaments on contaminated removed teeth," Drug Invention Today, vol. 10, no. 2, pp. 3026–3029, 2018.

R. Halkai, M. H. Hegde, and K. Halkai, "Enterococcus faecalis can survive extreme challenges: an overview," Nitte University Journal of Health Science, vol. 2, no. 3, pp. 49–53, 2012.