Comparative evaluation of antimicrobial efficacy of different endodontic sealers against Enterococcus Faecalis
An in-vitro study
Keywords:
Agar diffusion test, Enterococcus faecalis, inhibition zoneAbstract
Aim: The aim and objective of this invitro study was to determine antimicrobial activity of root canal sealer (AH Plus, Sealapex, MTA Fillapex) against Enterococcus faecalis. Materials and Methods: Three different commercially available root canal sealers namely - AH-Plus (Dentsply), MTA Fillapex (Angelus, bioceramic sealer) and Sealapex sealer (Kerr) were evaluated for their antimicrobial potential against Enterococcus faecalis (E.faecalis )using agar diffusion method. Wells were formed in the agar plates by removing agar at equal distance and then, filled with the endodontic sealers- AH Plus, Sealapex and MTA Fillapex. Standard antibiotic disc of co-trimoxazole was kept as a control. Petri dishes were inoculated at 37 ℃ at a time interval of 24 and 48 hours. The diameter of growth of inhibition zone were measured by using Antibiotic zone scale (HIMEDIA). The difference between the groups were evaluated by one -way ANOVA and intergroup evaluation was done by Tukey’s post hic test. Result: AH-Plus sealer showed larger zone of inhibition as compared to sealapex and MTA Fillapex sealer against E.faecalis at 24 and 48 hours. Co- trimoxazole used as control exhibited the highest antimicrobial efficacy against E.faecalis at 24 and 48 hour. MTA Fillapex showed least antimicrobial action.
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