Lip L protein antibodies
A new promising diagnostic marker for tuberculosis
Keywords:
Lipolytic enzymes, Tuberculosis (TB), IgG antibodiesAbstract
Background: Tuberculosis (TB) is as yet quite possibly the most over the top dreadful sickness, primarily in Southeast Asia, with the highest incidence in five countries i.e. India, Indonesia followed by China, Philippines, and Pakistan (in that order). In order to combat the rising tuberculosis (TB) load, new diagnostic tests are urgently needed. Because immunoassays are fast, quite simple, and cheap and may suggest the prospect of identifying cases overlooked by routine sputum smear microscopy, an efficient in vitro diagnostic test of tuberculosis based on serological approaches would be considered as an appealing advancement. Lipolytic enzymes, like lipases and esterases, are more important for mycobacterial survival. Lipolytic enzymes, including as lipases and phospholipases, have been found to help M. tuberculosis access host lipids. The main purpose of the current study was to compare the level of antibodies against membrane bound lipolytic enzymes in Tuberculosis patients and control subjects. Methods: To detect serum IgG antibodies against lipolytic enzymes, recombinant proteins were created and employed in an ELISA test. LipL antibody assay were done in serum samples of patients of tuberculosis (PTB, EPTB & MDR TB) and healthy controls.
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