In-silico and in-vitro investigation of phytoconstituents present in schleichera oleosa

An enzyme based anti-hyperlipidemic activity

Abstract

Hyperlipidemia is a common condition among today's generation. Only a few medicines are available for treatment, but they pose a risk if used for an extended period of time. As a result, the objective of this study is to find a potential HMG-CoA reductase inhibitor in a natural traditional plant, Schleichera oleosa. The extract was prepared through the cold maceration of the tree's bark with methanol. The preliminary phytochemical assessment was carried out in accordance with the standard test. The HMG-CoA reductase inhibition test kit was used as an in-vitro investigation to investigate the inhibitory potential of phytoconstituents at 2, 10, 20, 40, 60, 80, and 100 µg/ml. For 600 seconds, spectrophotometric scans (340 nm) were performed at 30 second intervals. The in-silico ligand-protein docking approach was used to determine the binding potential of phytoconstituents to a complex of the catalytic part of human HMG-CoA reductase (1HW8). The presence of triterpenes, phenols, tannins, glycosides, carbohydrates, and sterols was found, but flavonoids, alkaloids, and saponins were absent. The phytoconstituents were shown to be effective in the inhibition experiment, with 63.14 ± 0.34 % inhibition at 100 µg/ml, and the extract's IC50 was 54.87 µg/ml.