In Vitro anticholinesterase and antioxidant activity of Thunbergia coccinea leaves extract

P Gayathri; D Chandra Prabha

doi:10.53730/ijhs.v6nS2.8116

Authors

Gayathri. P
gayathrip021996@gmail.com
Research Scholar, Department of Biochemistry, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, Tamilnadu, India
D Chandra Prabha Associate Professor, Department of Biochemistry, Sri Ramakrishna College of Arts and Science for Women, Coimbatore, Tamilnadu, India

Keywords:

Thunbergia coccinea, cholinesterase inhibition, antioxidant activity, ethnomedicinal plant

Abstract

Herbal medicines gained an interest in the development of new drugs as a therapeutic agent for various manifestation. Relevant research reveals that Thunbergia coccinea is a plant which possess antipyretic, anti-inflammatory activity and it has been used as an ethnomedicinal plant. The present study explores the cholinesterase inhibitory potential relevant to neurodegenerative disorder and antioxidant activities of hydroethanolic extract of Thunbergia coccinea leaves. Cholinesterase inhibition was determined by Ellman method at different concentrations. The antioxidant activity was assessed by DPPH, FRAP, ABTS, TRAP, NO radical inhibition assay, Phosphomolybdate assay for total antioxidant capacity, total phenolic content, SOD scavenging activity. The hydroethanolic extract of Thunbergia coccinea leaves express significant amount of antioxidant and anticholinesterase activity against neurodegenerative disorders.

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References

M. Ekor, “The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety,” Frontiers in Pharmacology, vol. 4, p.177, 2014.

Rahul Chandran, Heidi Abrahamse, “Identifying Plant-Based Natural Medicine against Oxidative Stress and Neurodegenerative Disorders”, Oxidative Medicine and Cellular Longevity, vol. 2020, Article ID 8648742, 9 pages, 2020. https://doi.org/10.1155/2020/8648742.

Rouch, I., Dorey, JM., Boublay, N. et al. Personality, Alzheimer's disease and behavioural and cognitive symptoms of dementia: the PACO prospective cohort study protocol. BMC Geriatr 14, 110 (2014). https://doi.org/10.1186/1471-2318-14-110

Serrano-Pozo, Alberto & Frosch, Matthew & Masliah, Eliezer & Hyman, Bradley. (2011). Neuropathological Alterations in Alzheimer Disease. Cold Spring Harbor perspectives in biology. 3. a006189. 10.1101/cshperspect.a006189.

Uddin MS, Stachowiak A, Mamun AA, Tzvetkov NT, Takeda S, Atanasov AG, Bergantin LB, Abdel-Daim MM, Stankiewicz AM. Autophagy and Alzheimer's Disease: From Molecular Mechanisms to Therapeutic Implications. Front Aging Neurosci. 2018 Jan 30;10:04. doi: 10.3389/fnagi.2018.00004. PMID: 29441009; PMCID: PMC5797541.

Nwidu, L., Elmorsy, E., Aprioku, J., Siminialayi, I., & Carter, W. (2018). In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria. Medicines, 5(3), 71. https://doi.org/10.3390/medicines5030071

Sultana, N., & Das, S. (2019). Preliminary Phytochemical Screening and In Vitro Antimicrobial Activity of the leaf extract OF Thunbergia coccinea (Family-Acanthaceae). International Journal of Current Pharmaceutical Research, 11(4), 111–114. https://doi.org/10.22159/ijcpr.2019v11i4.34938

Evans, W.C.. (2009). Trease and Evans' Pharmacognosy: Sixteenth Edition.

Ellman, G. L., Courtney, K. D., Andres, V., Jr, & Feather-Stone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7, 88–95. https://doi.org/10.1016/0006-2952(61)90145-9

Khan, R.A., Khan, M.R. & Sahreen, S. Brain antioxidant markers, cognitive performance and acetylcholinesterase activity of rats: efficiency of Sonchus asper. Behav Brain Funct 8, 21 (2012). https://doi.org/10.1186/1744-9081-8-21

Singleton, V.L. and Rossi, J.A. (1965) Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagent. American Journal of Enology and Viticulture, 16, 144-158.

BLOIS, M. Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181, 1199–1200 (1958). https://doi.org/10.1038/1811199a0.

Sathish Kumar, T., Shanmugam, S., Palvannan, T., Bharathi Kumar, V. (2010). Evaluation of Antioxidant Properties of Elaeocarpus ganitrus Roxb. Leaves. Iranian Journal of Pharmaceutical Research, Volume 7(Number 3), 211-215. doi: 10.22037/ijpr.2010.767.

Ahmed, D., Khan, M. M., & Saeed, R. (2015). Comparative Analysis of Phenolics, Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants (Basel, Switzerland), 4(2), 394–409. https://doi.org/10.3390/antiox4020394.

Pellegrini, N., Serafini, M., Salvatore, S., Del Rio, D., Bianchi, M., & Brighenti, F. (2006). Total antioxidant capacity of spices, dried fruits, nuts, pulses, cereals and sweets consumed in Italy assessed by three different in vitro assays. Molecular nutrition & food research, 50(11), 1030–1038. https://doi.org/10.1002/mnfr.200600067

Umamaheswari, M & Chatterjee, T.K.. (2007). In Vitro Antioxidant Activities of the Fractions of Coccinia Grandis L. Leaf Extract. African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines. 5. 61-73. 10.4314/ajtcam.v5i1.31258.

Pilar Prieto et al., Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E, Analytical Biochemistry, Volume 269, Issue 2, 1999, Pages 337-341, ISSN 0003-2697, https://doi.org/10.1006/abio.1999.4019.

Guno Sindhu Chakraborthy., Free radical scavenging activity of Aesculus indica leaves, International Journal of Partech Research; 2009 1 (3): 524-526.

Wink, M. Introduction: Biochemistry, Physiology and Ecological Functions of Secondary Metabolites. In Annual Plant Reviews; Wink, M., Ed.; Wiley-Blackwell: Oxford, UK, 2010; Volume 40.

Melo, J. B., Agostinho, P., & Oliveira, C. R. (2003). Involvement of oxidative stress in the enhancement of acetylcholinesterase activity induced by amyloid beta-peptide. Neuroscience research, 45(1), 117–127. https://doi.org/10.1016/s0168-0102(02)00201-8

Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X., Kong, M., Li, L., Zhang, Q., Liu, Y., Chen, H., Qin, W., Wu, H., & Chen, S. (2016). An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules, 21(10), 1374. https://doi.org/10.3390/molecules21101374

Olamide E. Adebiyi, Funsho O. Olayemi, Tan Ning-Hua, Zeng Guang-Zhi, In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia, Beni-Suef University Journal of Basic and Applied Sciences, Volume 6, Issue 1, 2017, Pages 10-14, ISSN 2314-8535, https://doi.org/10.1016/j.bjbas.2016.12.003.

Stratil, P., Klejdus, B., & Kubán, V. (2006). Determination of total content of phenolic compounds and their antioxidant activity in vegetables--evaluation of spectrophotometric methods. Journal of agricultural and food chemistry, 54(3), 607–616. https://doi.org/10.1021/jf052334j

Jiménez-Estrada, M., Velázquez-Contreras, C., Garibay-Escobar, A. et al. In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico. BMC Complement Altern Med 13, 12 (2013). https://doi.org/10.1186/1472-6882-13-12

Osawa T. Novel natural antioxidants for utilization in food and biological system. pp. 241–251. In: Postharvest Biochemistry of Plant Food Materials in the Tropics. Uritani I, Garcia VV, Mendoza EM (eds). Japan Scientist Societies Press, Tokyo, Japan (1994)

Hua-Bin Li, Chi-Chun Wong, Ka-Wing Cheng, Feng Chen. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT - Food Science and Technology. Volume 41, Issue 3., 2008, Pages 385-390. ISSN 0023-6438. https://doi.org/10.1016/j.lwt.2007.03.011

Victoria, S & Das, S & Lalhlenmawia, H & Shantabi, Longjam & Sarda, S. (2014). ANTIOXIDANT ACTIVITIES OF THE LEAVES OF THUNBERGIA COCCINEA WALL.

Munteanu, I. G., & Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. International journal of molecular sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380

Kalpana, Duraisamy & Velmurugan, Natarajan & Wahab, Rizwan & Cho, Jae & Hwang, I. & Lee, Yang. (2012). GC-MS Analysis and Evaluation of Antimicrobial, Free Radical Scavenging and In Vitro Cytotoxic Activities of the Methanolic Extract of Rheum Undulatum. Science of Advanced Materials. 4. 1238-1246. 10.1166/sam.2012.1381.

S.C. Shen, W.R. Lee, H.Y. Lin, H.C. Huang, Ko Ch, L.L. Yang., In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E(2) production, Eur J Pharmacol, 446 (2002), pp. 187-194

Nathan C. (1992). Nitric oxide as a secretory product of mammalian cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 6(12), 3051–3064.

B., Mahesh & Shekar, Kokila & Kenganora, Mruthunjaya. (2020). EXPLORATION OF BIOACTIVE COMPONENTS OF THUNBERGIA COCCINEA, ITS PHARMACOGNOSTIC, ANTIOXIDANT, GCMS AND ANTIHYPERGLYCEMIC STUDIES. International Journal of Pharmacy and Pharmaceutical Sciences. 12. 37290. 10.22159/ijpps.2020v12i6.37290.

Khatun, Mahfuza & Islam, Ekramul & Islam Ph.D., Md Rafikul & Rahman, Aziz & Alam, Khurshid & Khondkar, Proma & Rashid, Mamunur & Parvin, Shahnaj. (2013). Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC research notes. 6. 121. 10.1186/1756-0500-6-121.

Damanbhalang Rynjah, & Sumit Das. (2020). In vitro anti-oxidation activity studies of the whole plant (except flower) of Thunbergia coccinea. Journal of Applied Pharmaceutical Research, 8(4), 45-49. https://doi.org/10.18231/j.joapr.2020.v.8.i.4.45.49

Roberta Re, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang, Catherine Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radical Biology and Medicine, Volume 26, Issues 9–10, 1999, Pages 1231-1237, ISSN 0891-5849, https://doi.org/10.1016/S0891-5849(98)00315-3.

Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016;15(1):71. Published 2016 Jul 25. doi:10.1186/s12937-016-0186-5.

Robak, J., & Gryglewski, R. J. (1988). Flavonoids are scavengers of superoxide anions. Biochemical pharmacology, 37(5), 837–841. https://doi.org/10.1016/0006-2952(88)90169-4.

Damanbhalang Rynjah, & Sumit Das. (2020). In vitro anti-oxidation activity studies of the whole plant (except flower) of Thunbergia coccinea. Journal of Applied Pharmaceutical Research, 8(4), 45-49. https://doi.org/10.18231/j.joapr.2020.v.8.i.4.45.49.