In-vitro antioxidant activity of hydroalcoholic extract of crinum latifolium using different method
Keywords:
crinum latifolium, quantitative phytochemical, antioxidant activityAbstract
The purpose of this research is to look for phytochemicals and determine the antioxidant potential of C. latifolium aerial parts. The qualitative analysis of several phytochemical components was determined using a well-known test methodology. The 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay, hydrogen peroxide, and nitric oxide radical scavenging method were used to investigate the antioxidant activity of a hydro ethanol extract of Crinum latifolium bark in vitro. Phytochemical studies discovered the existence of phenols and flavonoids. The extract demonstrated dose-dependent free radical scavenging capabilities in the models studied. For the DPPH method, aerial portions of Crinum latifolium extract had an IC50 value of 46.35µg/ml, which was close to that of ascorbic acid (IC50=20.03g/ml). The IC50 value for the Superoxide radical scavenging assay was determined to be 60.94µg/ml, which is comparable to ascorbic acid (IC50= 20.91g/ml). The IC50 value was determined to be 50.30µg/ml in reducing power determination, which was close to that of ascorbic acid (IC50=20.84g/ml). The phytochemical profile and antioxidant activity of Crinum latifolium, which will be exploited for medicinal purposes, are described in this paper.
Downloads
References
Halliwell B, Gutteridge JM. Oxygen toxicity, oxygen radicals, transition metals and diseases. Biochem J 1984;219:1-4.
Maxwell SR. Prospect for the use of antioxidant therapies. Drugs 1995;49:45-361.
Rice-Evans CA, Miller NJ, Paganga G. Structure antioxidant activity relationships of flavonoids and phenolic acids. Free RadicBiol Med 1996;20:933-56.
Prior RL, Cao G. Variability in dietary antioxidant related natural product supplements: The need for methods of standardization. J Am Nutraceutical Assoc 1999;2:46-56.
Dhawan BN. Centrally acting agents from Indian plants. In: Koslow SH, Murthy RS,Coelho GV, editors. Decade of the Brain: India/USA Research in Mental Health and Neurosciences. Rockville: National Institute of Mental Health; 1995. p. 197-202.
Sainkhediya J, Ray S. Studies on the threatened ethnomedicinal plants used by tribals of harda district of MP. Int J Sci Res 2014;3:2590-3.
Kirktikar KR, Basu BD. Indian medicinal plants, international book distributors. Vol. I. Dehradun, India; 1987.
Tambekar DH, Dahikar SB. Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens. J Adv Pharm Tech Res 2011;2:24-9.
Joshi S, Parkhe G, Aqueel N, Dixit N, Jain DK. Estimation of total phenolic, total flavonoids and total protein content of hydroalcoholic extract of Anacyclus pyrethrum. Pharmacologyonline 2019; 1:27-33.
Pradhan A, Jain P, Pal M, Chauhan M, Jain DK. Qualitative and quantitative determination of phytochemical contents of hydroalcoholic extract of salmaliamalabarica. Pharmacologyonline 2019; 1:21-26.
ParkheG, JainP. Study of antioxidantpotential of hydroalcoholic extract of Anethumgraveolens. Career.IntJSci Technol.2018;1(2):39-45.
Robak J and Gryglewski RJ. 1998. Flavonoids are scavenger of superoxide anions. BiochemPharmacol. 37: 837-841.
Chanda S and Dave R. 2009. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Afr J Microbiol Res. 3: 981-996.
S. Luqman, S. Srivastava, R. Kumar, A. K. Maurya, and D. Chanda, “Experimental assessment of Moringaoleifera leaf and fruit for its antistress, antioxidant, and scavenging potential using in vitro and in vivo assays,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 519084, 2012.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
