Antioxidant and antibacterial response of hydroalcoholic extract of Plumeria alba leaves

Chon Chee Siang; Nagaraja Suryadevara; Chandrasekaran Selvaraj; V. V. S. S. Appalaraju; Usha Kumari; Sakshi Bajaj

doi:10.53730/ijhs.v6nS1.8703

Authors

Chon Chee Siang Faculty of Pharmacy, AIMST University, Kedah 08100, Malaysia
Nagaraja Suryadevara Faculty of Medicine, Bioscience and Nursing MAHSA University, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia
Chandrasekaran Selvaraj Faculty of Medicine, Bioscience and Nursing MAHSA University, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia
V. V. S. S. Appalaraju Faculty of Pharmacy, MAHSA University, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia
Usha Kumari Faculty of Medicine, AIMST University, Kedah 08100, Malaysia
Sakshi Bajaj Delhi Institute of Pharmaceutical Science and Research (DIPSAR), Delhi Pharmaceutical Science and Research University (DPSRU), Pushp Vihar, New Delhi 110017, India

Keywords:

Plumeria alba, total phenolic content, total flavonoid content, gallic acid, antioxidant

Abstract

Plants are the natural treasure of antioxidants and antimicrobial agents. Present study was intended to evaluate the antioxidant and antimicrobial potential of Plumeria alba leaves hydroalcoholic extract (PALHE). Study involved the phytochemical screening, antioxidant, and antibacterial activity of PALHE. The PALHE was prepared using 95% ethanol as solvent through maceration method. The antioxidant activity involved determination of total phenolic content (TPC) and total flavonoid content (TFC). DPPH (1,1-Diphenyl2-picryl hydrazyl) radical scavenging assay was used for the determination of antioxidant activity of PALHE. The PALHE was investigated for its antibacterial activity against S. aureus and E. coli using well diffusion method. Various phytochemical screening tests were carried out and the results revealed the presence of carbohydrates, reducing sugar, mucilage, proteins, steroids, volatile oil, tannins, phenolic and flavonoids in the PALHE. Besides, moderate antioxidant activity was also revealed through the result of DPPH assay over the ethanolic leaves extract of the PALHE, where the IC50 was found to be 23.96 mcg/ml. Additionally, the TPC and TFC were found to be 71.04 mg (GAE/g of total phenol in terms of gallic acid equivalent) and 75.60 mg (RE/g of total flavonoid in terms of rutin equivalent) respectively.

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References

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Jaju S, Pahwa S, Sangita K, Fuloria N. Pharmacognostical Studies & antibacterial activity of leaves of Murraya Koenigii”, Pharmacognosy Journal. 2009; 1(3): 211.

Radha R, Kavimani S, Ravichandran V. Antitumour activity of methanolic extract of Plumeria alba L. leaves against Dalton lymphoma ascites in mice. International Journal of Health Research. 2008;1(2):79-85.

Choudhary M, Kumar V, Gupta P, Singh S. Investigation of antiarthritic potential of Plumeria alba L. leaves in acute and chronic models of arthritis. BioMed research international. 2014 1;2014.

Lawal OA, Ogunwande IA, Opoku AR. Constituents of essential oils from the leaf and flower of Plumeria alba grown in Nigeria. Natural Product Communications. 2014 ;9(11):1934578X1400901121.

Nor MM, Susanti D, Omar MN. Extraction And Phytochemical Screening From Different Parts Of Franggipani (Plumeria alba).

Porwal O, Nee JLJ, Fuloria S, ozdemir M, Kala D, Answer ET, Fuloria NK. Response Of Hydroalcoholic Extract Of Plumeria alba Leaves Against Periodontal Disease Triggering Microbiota. NVEO – Natural Volatiles & Essential Oils. 2021: 8(1): 13047.

S Vetriselvan, J.Shankar, S.Gayathiri, S.Ishwin, C.Hemah Devi, A.Yaashini, G.Sheerenjet: Comparative evaluation of in vitro antibacterial and antioxidant activity using standard drug and polyherbal formulation. International Journal of Phytopharmacology. 2012; 3(2): 112-116.

S Jaju, AT Patil, NJ Durgakar, NK Fuloria. Phytochemical and antimicrobial activity of stem and leaves of Desmodium gangeticum linn. Hamdard medicus. 2009; 52(4), 131-135.

Nor MM, Zuberdi AM, Omar MN. Evaluation Of The Total Phenol Content And Antioxidant Potential Of Plant Extract (Plumeria alba).

Chattopadhyay A, Dixit B, Nijhawan P, Kamarudheen N, Rao B. Phytochemical screening, in vitro anti quorum sensing activity and antioxidant activity of extracts of Plumeria alba, Pisonia alba and Cynodon dactylon. Journal of Applied Pharmaceutical Science. 2017 ;7(02):162-6.

Rahman H, Reddy VB, Ghosh S, Mistry SK, Pant G, Sibi G. Antioxidant, cytotoxic and hypolipidemic activities of Plumeria alba L. and Plumeria rubra L. American Journal of Life Sciences. 2014 17;2(6-1):11-5.

Balaji K, Ni LH, Rajindran B, Sikarwar MS, Fuloria NK, and Fuloria S. Determination of Total Phenolic, Flavonoid Content and Antioxidant Activity of Terminalia Chebula (Fruit). Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2015; 6(2): 413.

Fuloria S, Fuloria NK, Sundram KM, Sathasivam K., Singh S, Gupta K, Jain A, Sridevi U, HImaja M, Shanker S. Synthesis & discerning of antibiotic potential of PCMX based novel azetidinones. Acta Poloniae Pharmaceutica: Drug Research. 2017; 76(6): 171.

Fuloria NK, Fuloria S, K Sathasivam, S. Karupiah. Synthesis and discerning of antimicrobial potential of novel oxadiazole derivatives of chloroxylenol moiety. Acta Poloniae Pharmaceutica: Drug Research. 2017; 74(6): 1125.

Rohane SH, Chauhan AJ, Fuloria NK, Fuloria S. Synthesis and invitro antimycobacterial potential of novel hydrazones of eugenol. Arabian Journal of Chemistry. 2020; 13(2): 4495. https://doi.org/10.1016/j.arabjc.2019.09.004

Fuloria NK, Singh V, Yar MS, and Ali M. Synthesis, characterization and antimicrobial evaluation of novel imines and thiazolidinones. Acta Poloniae Pharmaceutica-Drug Research. 2009; 66(2): 141.

Gupta R, Fuloria NK, and Fuloria S. Synthesis and antimicrobial profile of some newer 2-amino-thiazole derivatives. Turkish Journal of Pharmaceutical Sciences. 2013; 10(3): 425.

Fuloria NK, Singh V, Shaharyar M, Ali M. Synthesis and antimicrobial evaluation of newer oxadiazoles derived from phenylpropiono hydrazides. Molecules. 2009; 14(5): 1898. https://doi.org/10.3390/molecules14051898

A Husain, Varshney MM, Percha V, Fuloria NK. Synthesis, characterization and biological evaluations of some 5-(substituted amino alkyl)-2-{(1, 3- benzothiazole-2-yl)}-thiazolidine-4 one Mannich bases as potent antibacterial agent. Journal of Applied Pharmaceutical Science 201; 3(4): 135.

Fuloria NK, Singh V, Yar MS, and Ali M. Antimicrobial evaluation of imines and thiazolidinones derived from 3-phenyl propanehydrazide. Acta Poloniae Pharmaceutica-Drug Research. 2009; 66(4): 371.

Fuloria NK, Singh V, Yar MS, and Ali M. Synthesis, characterization and biological studies of novel imines and azetidinone derivatives of haloaryloxy moiety. Asian Journal of Chemistry. 2008; 20(6), 4891.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949

Pahwa S, Fuloria NK, Kumar N, Singh V, Fuloria S. Diversified beauty of Saccharomyces boulardii. Pharmaceutical reviews. 2007, 5(6).

Jiea CK, Fuloria S, Subrimanyan V, Sathasivam K, Meenakshi DU, Kumar V, Chakravarthi S, Kumari U, Sekar M, Wu YS, Fuloria NK. Phytochemical screening and antioxidant activity of Cananga odorata extract. Research Journal of Pharmacy and Technology. 2022; 15(3), 1230.

Sa’ad MA, Kavitha R, Fuloria S, Fuloria NK, Ravichandran M, Lalitha P. Synthesis, Characterization and Biological Evaluation of Novel Benzamidine Derivatives: Newer Antibiotics for Periodontitis Treatment. Antibiotics. 2022, 11(2):207.