DNA damage assessment of Hela cancer cell line by biosynthetic zinc oxide nanoparticles
Keywords:
biosynthetic, green nanoparticles, cancer cell, hela, GC-MSAbstract
The current study examined the genotoxic effect of the biosynthetic zinc oxide nanoparticles ZnONPs using the Vitex agnus-castus plant, which contains effective compounds that inhibit the growth of pure cancer cells. The phenotypic and structural characteristics of the biosynthesized nanoparticles were detected using scanning electron microscopy (SEM). Most of the ZnONPs were dense and spherical with sizes ranging from (20-61) nanometers, on the cervical cancer cell line Hela and compared with the normal line Human Foreskin Fibroblast cells (HFF) using green dye. Comet assay technique and genotoxicity experiments were carried out at the Iraqi Center for Cancer Research / Al-Mustansiriya University. The results of this study showed the presence of damage to the DNA of cervical cancer cells Hela, which stopped cell division and entry. Cells are in the stage of programmed death a result of the release of reactive oxygen radicals (ROS) and changing cell functions by linking the nanomaterial to the genetic material. This gives hope for discovering a successful treatment to eliminate cervical cancer Hela or any type of cancer using methods Biosynthesis of nanoparticles.
Downloads
References
Thandra, K. C., Barsouk, A., Saginala, K., Aluru, J. S., & Barsouk, A. (2021). Epidemiology of lung cancer. Contemporary Oncology, 25(1), 45.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063897/
Castle, P. E., Einstein, M. H., and Sahasrabuddhe, V. V. (2021). Cervical cancer prevention and control in women living with human immunodeficiency virus. CA: a cancer journal for clinicians.https://acsjournals.onlinelibrary.wiley.com/doi/full/10.3322/caac.21696
Novio, F. (2020). Design of Targeted Nanostructured Coordination Polymers (NCPs) for Cancer Therapy. Molecules, 25(15), 3449.https://www.mdpi.com/1420-3049/25/15/3449.
Ziad T. Khodair, Mohanad W. Mahdi Alzubaidy, Asmaa M. Salih Almohaidi, Ammar Ahmed Sultan, Sana M. H. AL-Shimmary, and Sarah S. Albusultan.(2019). Synthesis of copper oxide nanoparticles (CuO-NPs) and its evaluation of antibacterial activity against P. aeruginosa biofilm gene’s. Technologies and Materials for Renewable Energy, Environment and Sustainability AIP Conf. Proc. 2190, 020006-1–020006-7; https://doi.org/10.1063/1.5138492 Published by AIP Publishing. 978-0-7354-1937-7/.
Mohanad W. Mahdi Alzubaidy, Asmaa M. Salih Almohaidi, Ammar Ahmed Sultan, and Sana M. H. ALShimmary.(2019).Virulence gene of Pseudomonas aeruginosa with nanoparticle. AIP Conf. Proc. 2123, 020039-1–020039-9; https://doi.org/10.1063/1.5116966 Published by AIP Publishing. 978-0-7354-1863-9/.
Bloh, A. H., Naji, H. K., & Obead, A. R. (2021). Novelty Predictor ZnO Nano practical for Cancer Treatment. Indian Journal of Forensic Medicine & Toxicology, 15(4). https://web.p.ebscohost.com/abstract?direct=true&profile=ehost&scope=site&authtype=crawler&jrnl=09739122&AN=153355850&h=RgSgmJxx3BbR239j%2bw2oC9P%2bT8Irx2RdrNLucgtUym0547OAUu%2fgNRvZ0HflG6OGykImI%2fkv19GsL3DlV3FGxQ%3d%3d&crl=c&resultNs=AdminWebAuth&resultLocal=ErrCrlNotAuth&crlhashurl=login.aspx%3fdirect%3dtrue%26profile%3dehost%26scope%3dsite%26authtype%3dcrawler%26jrnl%3d09739122%26AN%3d153355850 .
Hamrayev, H., Shameli, K., and Yusefi, M. (2020). Preparation of zinc oxide nanoparticles and its cancer treatment effects: A review paper. Journal of Advanced Research in Micro and Nano Engineering, 2(1), 1-11. https://www.akademiabaru.com/submit/index.php/armne/article/view/2962
Hamrayev, H., Shameli, K., & Korpayev, S. (2021). Green Synthesis of Zinc Oxide Nanoparticles and Its Biomedical Applications: A Review. Journal of Research in Nanoscience and Nanotechnology, 1(1), 62-74. https://www.akademiabaru.com/submit/index.php/jrnn/article/view/3595
Wu, H. and J. Zhang. (2018) "Chitosan-Based Zinc Oxide Nanoparticle for Enhanced Anticancer Effect in Cervical Cancer: A Physicochemical and Biological Perspective." Saudi Pharm J 26, no: 205-10. http://dx.doi.org/10.1016/j.jsps.2017.12.010
Latif, B. M. A., & Alzubaidy, M. W. M. (2021). Effect Of Zinc Oxide Nanoparticles On Activity Of Cell Line (B16) Causes Skin Cancer. NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, 472-478.
https://www.nveo.org/index.php/journal/article/view/3645
Pillai, A. M., Sivasankarapillai, V. S., Rahdar, A., Joseph, J., Sadeghfar, F., Rajesh, K., & Kyzas, G. Z. (2020). Green synthesis and characterization of zinc oxide nanoparticles with antibacterial and antifungal activity. Journal of Molecular Structure, 1211, 128107.
https://www.sciencedirect.com/science/article/abs/pii/S0022286020304324
Řiháček, T., Horák, M., Schachinger, T., Mika, F., Matějka, M., Krátký, S., ... & Müllerová, I. (2021). Beam shaping and probe characterization in the scanning electron microscope. Ultramicroscopy, 225, 113268.
https://www.sciencedirect.com/science/article/abs/pii/S0304399121000589
Mitteer, D. R., & Greer, B. D. (2022). Using GraphPad Prism’s Heat Maps for Efficient, Fine-Grained Analyses of Single-Case Data. Behavior Analysis in Practice, 1-10. https://link.springer.com/article/10.1007/s40617-021-00664-7
Ababutain, I. M., & Alghamdi, A. I. (2021). In vitro anticandidal activity and gas chromatography-mass spectrometry (GC-MS) screening of Vitex agnus-castus leaf extracts. PeerJ, 9, e10561.https://peerj.com/articles/10561/
Gur, T., Meydan, I., Seckin, H., Bekmezci, M., & Sen, F. (2022). Green synthesis, characterization and bioactivity of biogenic zinc oxide nanoparticles. Environmental Research, 204, 111897. https://www.sciencedirect.com/science/article/abs/pii/S0013935121011920
Jiang, J., Pi, J., and Cai, J. (2018). The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorganic chemistry and applications. https://www.hindawi.com/journals/bca/2018/1062562/
Li, F., Song, L., Yang, X., Huang, Z., Mou, X., Syed, A., ... & Zheng, L. (2020). Anticancer and genotoxicity effect of (Clausena lansium (Lour.) Skeels) Peel ZnONPs on neuroblastoma (SH-SY5Y) cells through the modulation of autophagy mechanism. Journal of Photochemistry and Photobiology B: Biology, 203, 111748. https://www.sciencedirect.com/science/article/abs/pii/S1011134419315490.
Mohanad W. Mahdi Alzubaidy. (2022). Evaluation the Breast Cancer Cell Line DNA Damage by Biosynthetic Silver-Green Nanoparticles. Texas Journal of Agriculture and Biological Sciences, 3, 14–19. Retrieved from https://zienjournals.com/index.php/tjabs/article/view/1254
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). The COVID-19 pandemic. International Journal of Health Sciences, 5(2), vi-ix. https://doi.org/10.53730/ijhs.v5n2.2937
Thaib, P. K. P., & Rahaju, A. S. (2022). Clinicopathological profile of clear cell renal cell carcinoma. International Journal of Health & Medical Sciences, 5(1), 91-100. https://doi.org/10.21744/ijhms.v5n1.1846
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
