Novel method for determination of Vitamins E and C by Cloud-point extraction
Keywords:
cloud point extraction method, vitamin E, vitamin CAbstract
A new, simple, fast and accurate spectrophotometric method has been found for the determination of vitamin E and C in their complexes by cloud point method. The Beer-Lambert Law was complied with for a range of concentrations ranging from 5-50 µg.ml-1 for vitamin E and 5-40 µg.ml-1 for vitamin C, and the value of the correlation coefficient R2: 9987 and 9997, and the value of the molar absorption coefficient Ԑ: 24421.25 and 10760.93, Sandel's sensitivity was 0.0176 μg.cm-2 and 0.0164 μg.cm-2 for vitamins E and C, respectively, while the detection limit was 0.0008 and 0.0009, and the quantitative limit was 0.0026 and 0.0030 for vitamins E and C, respectively. The method has been successfully applied for the determination of vitamin E and C in their pure forms and pharmaceutical preparations.
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