Polyhydroxybutyrate (PHB) production by Halomonas boliviensis isolated from waste water

https://doi.org/10.53730/ijhs.v6nS2.8961

Authors

  • Anuradha Singh Department of Biotechnology, Faculty of Engineering and Technology Rama University, Kanpur, Uttar Pradesh
  • Vivek Srivastava Department of Biotechnology, Faculty of Engineering and Technology Rama University, Kanpur, Uttar Pradesh

Keywords:

Halotolerant Spp., polyhydroxybutyrate (PHB), bioplastics

Abstract

Plastic materials are often utilized as plastic packaging and have various uses since their longevity and consistency. Plastic products are generally not biodegradable or persist in our surroundings, posing major harm to the ecology. Biopolymers, or bioplastics, are developing as a blessing in the fight against waste buildup. Polyhydroxybutyrate (PHB) is a biopolymer that can be used instead of manufactured polymers. PHB is a fatty storage substance that accumulates inside microbes' cell walls when stressed. Halophilic microbes can be highly useful in manufacturing PHB since they are cheap, and PHB extraction is considerably easy in halo-tolerant species. As a result, our research emphasizes the identification of PHB-generating halotolerant species of microbes using untreated wastewater. Nile blue A and Sudan Black B staining were used to identify PHB favourable strains. The effective microbe generated PHB on a large scale utilizing sewage as the medium.

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References

Anderson AJ, Dawes EA. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 1990;54:450–72.

Choi J, Lee SY (1999) Factors affecting the economics of poly-hydroxyalkanoate production by bacterial fermentation. Appl Microbiol Biotechnol 51:13–21

Fendrich C. Halovibrio variabilis gen. nov. sp. nov, Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid Eubacterium from Great Salt Lake, Utah, USA. Syst Appl Microbiol 1988;11:36–43.

Gede Budasi, I. & Wayan Suryasa, I. (2021). The cultural view of North Bali community towards Ngidih marriage reflected from its lexicons. Journal of Language and Linguistic Studies, 17(3), 1484–1497

Hezayen FF, Rehm BHA, Eberhardt R, Steinb¨ uchel A. Polymer production by two newly isolated extremely halophilic archaea: application of a novel corrosion-resistant bioreactor. Appl Microbiol Biotechnol 2000;54:319–25.

Kustina, K.T., Dewi, G.A.A.O., Prena, G.D., Suryasa, W. (2019). Branchless banking, third-party funds, and profitability evidence reference to banking sector in indonesia. Journal of Advanced Research in Dynamical and Control Systems, 11(2), 290-299.

Law JH, Slepecky RA. Assay of poly-hydroxybutyric acid. J Bacteriol 1961;82:33–6.

Lee SY (1996a) Bacterial polyhydroxyalkanoates. Biotechnol Bioeng 49:1–14

Lee SY (1996b) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol 14:431–438

Lee SY, Lee KM, Chang HN, Steinbüchel A (1994) Comparison of recombinant Escherichia coli strains for synthesis and accumulation of poly-(3-hydroxybutyric acid) and morphological changes. Biotechnol Bioeng 44:1337–1347

Lee SY, Wong HH, Choi J, Lee SH, Lee SC, Han CS. Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation. Biotechnol Bioeng 2000;68:466–70.

Lee SY. Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol 1996;14:431–8.

Lillo JG, Rodriguez-Valera F (1990) Effects of culture conditions on poly(β-hydroxybutyric) acid production by Haloferax mediterranei. Appl Environ Microbiol 56:2517–2521

Lingayya Hiremanth, Narendra Kumar S, Ravishankar H.N, Swathi Angadi and Sukanya P (2015). Design, Screening and Microbial Synthesis of Bio-polymers of Polyhydroxybutyrate from low cost carbon sources. International Journal of Advanced Research, Vol 3. Issue 2. 420-425. DOI: http:// www.journalijar.com/uploads/272_IJAR-5010

Martinez-Canovas MJ, Quesada E, Llamas I, Bejar V. Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 2004;54:733–7.

Niven DF, MacLeod RA. Sodium ion-proton antiport in a marine bacterium. J Bacteriol 1978;134:737–43.

Oren A. Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev 1999;63:334–48.

Oren A. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 2002;28:56–63.

Page WJ, Cornish A (1993) Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-β-hydroxybutyrate. Appl Environ Microbiol 59:4236–4244

Page WJ, Sherburne R, D’Elia L, Graham LL. Poly(-hydroxybutyrate) extrusion from pleomorphic cells of Azotobacter vinelandii UWD. Can J Microbiol 1995;41(Suppl. 1):22–31.

Patnayak, S. and Sree, A. (2005). Screening of Bacterial Associates of Marine Sponges for Single Cell Oil and PUFA. Lett Appl Microbiol. 40(5) p. 358 – 363. DOI:http://www.ncbi.nlm.nih.gov/pubmed/15836739/10.1111/j.1472-65X.2005.01671.x

Quesada E, B´ ejar V, Ferrer MR, Calvo C, Llamas I, Martinez-Canovas F, et al. Moderately halophilic, exopolysaccharide- producing bacteria. In: Ventosa A, editor. Halophilic microorganisms. Berlin: Springer; 2004. p. 297–314.

Quillaguamán J, Delgado O, Mattiasson B, Hatti-Kaul R (2006) Poly (β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1. Enzyme Microb Technol 38:148–154

Quillaguamán J, Guzmán H, Van-Thuoc D, Hatti-Kaul R (2009) Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects. Appl Microbiol Biotechnol (Accepted for publication).

Quillaguamán J, Hashim S, Bento F, Mattiasson B, Hatti-Kaul R (2005) Poly(β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1 using starch hydrolysate as substrate. J Appl Microbiol 99:151-157

Reddy CSK, Ghai R, Kalia V (2003) Polyhydroxyalkanoates: an overview. Bioresour Technol 87:137–146

Rodriguez-Valera F, Lillo JAG (1992) Halobacteria as producers of polyhydroxyalknoates. FEMS Microbiol Rev 103:181–186

Rodriguez-Valera F, Lillo JAG. Halobacteria as producers of poly-hydroxyalkanoates. FEMS Microbiol Rev 1992;103:181–6.

Spiekermann P, Rehm BHA, Kalscheuer R, Baumeister D and Steinbuchel A (1999). A sensitive, viable-colony staining method using nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol. 171, 73 – 80. DOI:10.1007/s00203005068

Steinbuchel A, Aerts K, Babel W, Folner C, Leibergesell M, Wieczorek R. Considerations on the structure and biochemistry of bacterial polyhydroxyalkanoic acid inclusions. Can J Microbiol 1995;41(suppl. 1):94–105.

Steinbüchel A, Füchtenbush B (1998) Bacterial and other biological systems for polyester production. Trends Biotechnol 16:419–427

Steinbuchel. A and Schubert. P. (1988) Expression of Alcaligenes eutrophus polyhydroxybutyric acid – synthetic pathway in Pseudomonas sp. Arch. Microbiol.153, 101-104.

Stuart ES, Tehrani A, Valentin HE, Dennis D, Lenz RW, Fuller RC. Protein organization on the PHA inclusion cytoplamic boundary. J Biotechnol 1998;64:137–44.

Vreeland RH, Anderson R, Murray RGE. Cell wall and phospholipid composition and their contribution to the salt tolerance of Halomonas elongata. J Bacteriol 1984;160:879–83.

Wang F, Lee SY (1997) Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation. Appl Environ Microbiol 63:3703–3706

Published

14-06-2022

How to Cite

Singh, A., & Srivastava, V. (2022). Polyhydroxybutyrate (PHB) production by Halomonas boliviensis isolated from waste water. International Journal of Health Sciences, 6(S2), 14934–14943. https://doi.org/10.53730/ijhs.v6nS2.8961

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Peer Review Articles