Development and validation of RP – HPLC method for quantitation of luliconazole in bulk and formulation
Keywords:
luliconazole, analytical method validation, HPLCAbstract
A new simple, specific, precise & validated Analytical Method has been developed for the determination of Luliconazole in bulk and pharmaceutical formulation. The separation was achieved on a C18 ODS (250×4.6 mm,5µm) or using mobile phase consisting of methanol: water (85:15) at a flow rate of 1.0 ml/min. Detection was carried out at 296nm. The retention time of Luliconazole was found to be 4.2min. the calibration curve found linear between range of 20-60 µg/ml with a regression coefficient of 0. 9998. The percentage recovery of Luliconazole was found to be in the range of 90-110%. The method was validated in accordance with International Conference on Harmonization. LOD & LOQ for Luliconazole were found to be 0.24μg & 0.748μg/ml respectively. Present method is simple, precise and can be used in routine analysis of Luliconazole in bulk and pharmaceutical lotion.
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