Fluoroquinolones resistance of Pseudomonas aeruginosa isolated from different clinical samples
Keywords:
Pseudomonas aeruginosa, resistance, fluoroquinolones, antibioticsAbstract
A total of 156 clinical specimens were collected from patients at Al-Sadder Medical City, Al-Furat al-Awsat Hospital, and the burn center between August 2021 and February 2022. According to medical service utilities, Burn center was the highest with total number of samples with 96 samples and the highest recovery rate 17 isolates followed by Alfurat Al-Awsat Hospital with 32 samples and 4 isolates and finally tame Al-Sadder Medical City with only 28 samples and 2 isolates. The results of 23 isolates of Pseudomonas aeruginosa revealed that the most effective agent was ciprofloxacin with (60%) resistance which represents regression in the overall potential while Norfloxacin showed high resistance percentage (73%) (The least effective among them) followed by Ofaloxacin (69%) and levofloxacin with (65%).genetically, the result of aac(6´)-Ib-cr gene showed that 21/23 isolates gave positive result with percentage (91.3%).
Downloads
References
Abdelrahman, D. N., Taha, A. A., Dafaallah, M. M., Mohammed, A. A., El Hussein, A. R. M., Hashim, A. I., ... & Altayb, H. N. (2020). β-lactamases (bla TEM, bla SHV, bla CTXM-1, bla VEB, bla OXA-1) and class C β-lactamases gene frequency in Pseudomonas aeruginosa isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study. F1000Research, 9.
Abdulameer, H. H., & Abdulhassan, G. A. (2021). Occurrence of Point Mutations in gyrA and parC Genes of Ciprofloxacin-Resistant Pseudomonas aeruginosa Isolated from Burn Infections. Iraqi Journal of Science, 3457-3466
Aboulfotoh Hashish, N. M., Gawad Abbass, A. A., & Khamis Amine, A. E. (2017). Pseudomonas aeruginosa in swimming pools. Cogent Environmental Science, 3(1), 1328841.
El-Badawy, M. F., Alrobaian, M. M., Shohayeb, M. M., & Abdelwahab, S. F. (2019). Investigation of six plasmid-mediated quinolone resistance genes among clinical isolates of pseudomonas: a genotypic study in Saudi Arabia. Infection and Drug Resistance, 12, 915.
Farahi, R. M., Ali, A. A., & Gharavi, S. (2018). Characterization of gyrA and parC mutations in ciprofloxacin-resistant Pseudomonas aeruginosa isolates from Tehran hospitals in Iran. Iranian journal of microbiology, 10(4), 242.
Hassan, J. S., Al-Safar, M. A., & Rhman, T. R. A. (2019). The Role of DNA Gyrase (gyrA) in Ciprofloxacin-Resistant Locally Isolates Pseudomonas aeruginosa in Al-Khadhmiya Teaching Hospital Baghdad, Iraq. Journal of Pure and Applied Microbiology, 13(1), 499-504
Hopkins, K. L., Davies, R. H., & Threlfall, E. J. (2005). Mechanisms of quinolone resistance in Escherichia coli and Salmonella: recent developments. International journal of antimicrobial agents, 25(5), 358-373.
Horcajada, J. P., Montero, M., Oliver, A., Sorlí, L., Luque, S., Gómez-Zorrilla, S., ... & Grau, S. (2019). Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections. Clinical microbiology reviews, 32(4), e00031-19.
Jacoby, G. A. (2005). Mechanisms of resistance to quinolones. Clinical infectious diseases, 41(Supplement_2), S120-S126.
Jones, M. E., Draghi, D. C., Thornsberry, C., Karlowsky, J. A., Sahm, D. F., & Wenzel, R. P. (2004). Emerging resistance among bacterial pathogens in the intensive care unit–a European and North American Surveillance study (2000–2002). Annals of Clinical Microbiology and Antimicrobials, 3(1), 1-11.
Karlowsky, J. A., Lagacé-Wiens, P. R., Simner, P. J., DeCorby, M. R., Adam, H. J., Walkty, A., ... & Zhanel, G. G. (2011). Antimicrobial resistance in urinary tract pathogens in Canada from 2007 to 2009: CANWARD surveillance study. Antimicrobial agents and chemotherapy, 55(7), 3169-3175.
Landman, D., Bratu, S., Kochar, S., Panwar, M., Trehan, M., Doymaz, M., & Quale, J. (2007). Evolution of antimicrobial resistance among Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae in Brooklyn, NY. Journal of antimicrobial chemotherapy, 60(1), 78-82.
Moghadasi, M., Mirzaee, M., & Mehrabi, M. R. (2016). Frequency of quinolone resistance and qnrb and qnrc genes in clinical isolates of Klebsiella pneumoniae. Journal of Medical Bacteriology, 5(5-6), 39-45.
Molapour, A., Peymani, A., Saffarain, P., Habibollah-Pourzereshki, N., & Rashvand, P. (2020). Plasmid-mediated quinolone resistance in Pseudomonas aeruginosa isolated from burn patients in Tehran, Iran. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders), 20(1), 49-55.
Moradali, M. F., Ghods, S., & Rehm, B. H. (2017). Pseudomonas aeruginosa lifestyle: a paradigm for adaptation, survival, and persistence. Frontiers in cellular and infection microbiology, 7, 39.
Muylaert, A., & Mainil, J. G. (2013). Fluoroquinolones resistances: the current situation. In Annales de Medecine Veterinaire (Vol. 157, No. 1, pp. 15-26).
Neuhauser, M. M., Weinstein, R. A., Rydman, R., Danziger, L. H., Karam, G., & Quinn, J. P. (2003). Antibiotic resistance among gram-negative bacilli in US intensive care units: implications for fluoroquinolone use. Jama, 289(7), 885-888.
Nouri, R., Ahangarzadeh Rezaee, M., Hasani, A., Aghazadeh, M., & Asgharzadeh, M. (2016). The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran. brazilian journal of microbiology, 47, 925-930.
PangZ, R., & GLICK, B. (2019). Antibioticresistancein Pseudomonasaeruginosa: mechanismsand alternativetheraG peuticstrategies. BiotechnolAdv, 37(1), 177G192.
Pitt, T. L., Sparrow, M., Warner, M., & Stefanidou, M. (2003). Survey of resistance of Pseudomonas aeruginosa from UK patients with cystic fibrosis to six commonly prescribed antimicrobial agents. Thorax, 58(9), 794-796.
Ragnar Norrby, S., & Lietman, P. S. (1993). Safety and tolerability of fluoroquinolones. Drugs, 45(3), 59-64.
Rajaei, S., Kazemi-Pour, N., & Rokhbakhsh-Zamin, F. (2017). Frequency of plasmid-mediated quinolone resistance genes among clinical isolates of Pseudomonas aeruginosa in Kerman, Iran. Iranian Journal of Medical Microbiology, 11(3), 10-18.
Rocha, A. J., Barsottini, M. R. D. O., Rocha, R. R., Laurindo, M. V., Moraes, F. L. L. D., & Rocha, S. L. D. (2019). Pseudomonas aeruginosa: virulence factors and antibiotic resistance genes. Brazilian Archives of Biology and Technology, 62
Saderi, H., Lotfalipour, H., Owlia, P., & Salimi, H. (2010). Detection of metallo-β-lactamase producing Pseudomonas aeruginosa isolated from burn patients in Tehran, Iran. Laboratory Medicine, 41(10), 609-612.
Saki, M., Farajzadeh Sheikh, A., Seyed-Mohammadi, S., Asareh Zadegan Dezfuli, A., Shahin, M., Tabasi, M., ... & Khani, P. (2022). Occurrence of plasmid-mediated quinolone resistance genes in Pseudomonas aeruginosa strains isolated from clinical specimens in southwest Iran: a multicentral study. Scientific Reports, 12(1), 1-8
Salman, A. D., Zainab, A., & Rahman, E. S. A. (2017). Bacteriological Study of Pseudomonas Aeruginosa Isolated from Different Infections and Study Antimicrobial Activities of Plant Extract Solanum Nigrum Against It. Iraqi journal of Science, 58(4C), 2278-2284.
Shaker, M. M., & Al-Hadrawi, H. A. N. (2021). Measuring the effectiveness of antibiotics against Pseudomonas aeruginosa and Escherichia coli that isolated from urinary tract infection patients in Al-Najaf city in Iraq. Materials Today: Proceedings.
Stehling, E. G., Leite, D. S., & Silveira, W. D. (2010). Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil. The Brazilian Journal of Infectious Diseases, 14(5), 462-467.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Szmolka, A., & Nagy, B. (2013). Multidrug resistant commensal Escherichia coli in animals and its impact for public health. Frontiers in microbiology, 4, 258.
Tohidpour, A., Najar Peerayeh, S., & Najafi, S. (2013). Detection of DNA gyrase mutation and multidrug efflux pumps hyperactivity in ciprofloxacin resistant clinical isolates of Pseudomonas aeruginosa. Journal of Medical Microbiology and Infectious Diseases, 1(1), 1-7.
Widana, I.K., Dewi, G.A.O.C., Suryasa, W. (2020). Ergonomics approach to improve student concentration on learning process of professional ethics. Journal of Advanced Research in Dynamical and Control Systems, 12(7), 429-445.
Williamson, K. S., Richards, L. A., Perez-Osorio, A. C., Pitts, B., McInnerney, K., Stewart, P. S., & Franklin, M. J. (2012). Heterogeneity in Pseudomonas aeruginosa biofilms includes expression of ribosome hibernation factors in the antibiotic-tolerant subpopulation and hypoxia-induced stress response in the metabolically active population. Journal of bacteriology, 194(8), 2062-2073.
Zaidan, M. R., Noor Rain, A., Badrul, A. R., Adlin, A., Norazah, A., & Zakiah, I. (2005). In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop biomed, 22(2), 165-170.
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.
