Metallothionein gene polymorphism is considered to be a risk factor for chronic diseases
Keywords:
Mutation, ARMS-PCR, Metalothionen, Kidney failureAbstract
Metallothioneins are considered the main proteins to protect the cell from toxins like heavy metals and oxidative stress encoded by the Metallothionein gene. Any mutation in this gene leads to a defect in the function of Metallothionein proteins, leading to an increase in the effect of heavy metals. Reactive oxygen species, the SNP A-G (rs28366003) in promotor of the metallothionein gene converts the A allele to G allele, and therefore decreases the transcription qualification of the Metallothionein gene; the aim of the study is correlation between A-G (rs28366003) polymorphism in promotor of Metallothionein gene and the risk factor as well as detecting the levels of heavy metals in patients with Kidney failure, this study includes 60 persons with Kidney failure and 20 persons as a control group with age gape (30-55) years, the blood sample collects from the subject and separate into two-tube, EDTA tube to extraction the DNA and Gel tube to biochemical test. The result showed that there is a relationship between polymorphism in the promotor of the Metallothionein gene in the site A-G (rs28366003). The distribution of different genotypes is wild A.A. 22 %, hetero AG 25%, and mutant 53% in the patients.
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