Comparative evaluation of periodontal ligament cell viability of permanent teeth in five different storage media followed by simulated avulsion injury: An in vitro study

Dharati Patel; Harsh Mistry; Chhaya Patel; Anvi Shah; Vrunda Parikh; Shreya Mistry

doi:10.53730/ijhs.v6nS5.9022

Authors

Dharati Patel Senior Lecturer, Department of Pediatric and Preventive Dentistry, Narsinhbhai Dental College and Hospital, Sankalchand Patel University, S.P. Sahakar Vidhyadham, Ambaji Gandhinagar Link Road, Near Kamana char rasta, Visnagar, Gujarat-384315
Harsh Mistry Senior Lecturer, Department of Pediatric and Preventive Dentistry, Narsinhbhai Dental College and Hospital, Sankalchand Patel University, S.P. Sahakar Vidhyadham, Ambaji Gandhinagar Link Road, Near Kamana char rasta, Visnagar, Gujarat-384315
Chhaya Patel Reader, Department of Pediatric and Preventive Dentistry, Karnavati School of Dentistry, Karnavati University, Uvarsad, Gandhinagar, Gujarat
Anvi Shah Senior Lecturer, Department of Pediatric and Preventive Dentistry, Ahmedabad Dental College and Hospital, Bhadaj, Ahmedabad, Gujarat-384315
Vrunda Parikh MDS, Associate Pediatric Dentist, Gujarat Multispeciality Dental Clinic, Gandhinagar, Gujarat
Shreya Mistry Senior Lecturer, Department of Periodontology, Karnavati School of Dentistry, Karnavati University, Uvarsad, Gandhinagar, Gujarat

Keywords:

Tooth Avulsion, Avulsion Storage Media, Periodontal Ligament Fibers, Reimplantation, Cell Viability, HBSS, Aloe Vera, Milk, Egg White, Coconut water

Abstract

Aim: To evaluate and compare the viability of periodontal ligament cells of avulsed teeth in five different storage media followed by simulated avulsion injury. Settings and Design: Seventy-five premolars extracted for orthodontic therapeutic purposes were randomly and equally ivided into five groups based on storage media used [A: HBSS (control), B: Milk (experimental), C: Aloe Vera (experimental), D: Egg white (experimental), E: Coconut water (experimental)].Methods and Material: Following extractions, the teeth were placed in one of the five different storage media for 60 minutes, following which the scrapings of the PDL were collected in Falcon tubes which already contained collagenase enzyme in 2.5 mL of Phosphate buffered saline. The tubes were subsequently incubated and centrifuged. Then acquired PDL cells were stained with Trypan Blue dye and were counted under an optical microscope. Statistical Analysis Used: Results were subjected to statistical analysis using ANOVA test and Post Hoc Tukey test. p-value < 0.05 is considered to be significant. Results: HBSS showed the highest percentage of viable cells (80.14%), followed by Egg white (74.01%) and Aloe Vera (73.68%). Milk and Coconut water showed the least percent of viable cells 63.20%, 63.58% respectively.

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