Development and validation of an RP-HPLC method for estimating nutraceutical lutein
Keywords:
Lutein, analytical method development, validation, nutraceutical, RP-HPLCAbstract
Background: Nutraceuticals have long been thought of as natural, safe supplements that can help prevent disease, replace prescription medications, compensate for poor diet, and boost overall health. One such nutraceutical is Lutein, a pigmented xanthophyll carotenoid, which offers a plethora of uses and health benefits. Lutein, like many carotenoids is a lipid soluble pigment which faces major solubility and bioavailability issues owing to its lipophilicity, due to which its estimation is indeed challenging. And the analysis of a simple solvent extract of Lutein is complicated and lacks any detailed method for its estimation. Additionally, literature review indicates use of very complicated mobile phases and tedious solvent extraction procedures involved in the estimation of Lutein. Objective: The manuscript describes the development and validation of a rapid, sensitive and specific Reverse Phase-High Performance Liquid Chromatographic (RP-HPLC) method involving Ultraviolet (UV) detection for the determination and quantification of nutraceutical Lutein in bulk and capsule dosage form.
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