Molecular characterization of some apricot Prunus armeniaca L. and plum Prunus domistica L. cultivars grown in northern Iraq using RAPD markers
Keywords:
apricot, plum, RAPD markers, molecular characteristicsAbstract
The current research aimed to use the Random Amplification Polymorphic DNA (RAPD) markers based on the polymerase chain reaction (PCR) technique to find genetic variation between six cultivars of Prunus armeniaca L. and Prunus domistica L planted in North Iraq. DNA was extracted from young plant leaves, Thirteen primers were used in RAPD reactions, and samples were migrated using electrophoresis using an agarose gel. The results showed that the concentration of the extracted DNA ranged between (25.8 - 66.3) μg/ml, and the results showed a discrepancy in the number and molecular size of the DNA bands for each cultivar, as the total number of bands reached (1038), of which 882 were polymorphic, and 156 monomorphic bands.
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References
Canli FA (2008). Progress in genetic mapping of Prunus species. Erciyes University J. Inst. Sci. Tech., 24(1-2): 414 – 424
Al-Mousawi, Ali Hussein (1987), Botany Classification Science, first edition, Ministry of Higher Education and Scientific Research, University of Baghdad, 379 p. (in Arabic)
Das B., N. Ahmed , P. Singh (2011) Prunus diversity- early and present development : A review . nternational Journal of Biodiversity and Conservation Vol. 3(14), pp. 721-734
Moussalli, Hossein Ali. 2000. Apricots, cultivation, varieties, pests, manufacture and preservation of their products. First edition. Aladdin House Publications. Syria.
Takeda, T., T. Shimada ,K.Nomura , T. ozaki.(1998) Classification of apricot varieties by RAPD analysis . J,Japan Soc. Hort . Sci .67(1):21-27
AL-apras, Nawras, Faisal Hamed, Stefano Badulusi and Ahmed Mohamed Muhanna. (2012) Artemisia herba-alba Asso using RAPD technology and its wild spread in the Qalamoun region - Syria. Arab Journal of Pharmaceutical Sciences - Journal of the Union of Arab Universities. 4 (8) pp. 390-396. (in Arabic)
Shehab, M. O. M. (2020). Taxonomic study of cultivars of the genus Rosaceae (Pyrus L.) cultivated in northern Iraq. PhD thesis - College of Education for Pure Sciences - University of Mosul. Iraq(in Arabic)
Al-Azzawi, Adnan Fadil, Aqeel Hussein Al-Assi, Sara Qahtan Soliman. (2013) Diversity Analysis of Some Cotton Varieties Gossypium hirsutum L. Using RADP Markers. Zagazig J. Agric. Res vol. 40 No. 4. (in Arabic)
Ercisli, S.; Agar,G.; Yildirim,N.; Esitken, A.;Orhan, E.( 2009) Identification of apricot cultivars in Turkey (Prunus armeniaca L.) using RAPD markers. Romanian Biotechnological Letters, 14, 4, 4582-4588 85- 196.
Nei, M. and Li, W. H. (1979). Mathmatical model for studying genetic variation in terms of restriction endonucleases, math. Acad. Sci, USA. 76: 5269- 5273
Sneath, P.H.; Sokal, R.R. (1973). "Numerical Taxonomy the Principles and Practice of Numerical Classification". WH. Freeman and Co., San Francisco. 573p.
Rohlf, F. J. (1993). Numerical taxonomy and multivariate analysis system. version 1-8. Exeter Software. Setauket. N.Y.
Casas A.M., E. Igartua, G. Balaguer, M.A. Moreno.(1999). Genetic diversity of Prunus rootstocks analyzed by RAPDS markers. Euphytica 110(2):139-149
Al-Samarrai, Rana Hashem Alloush, Talib Awaid Al-Khazraji, Khalil Ibrahim Abbas Al-Shammari (2014). Use RAPD indicators Random Amplification Polymorphic DNA and specialized gene RbcL in determining the genetic relationship between Among some genus of Lathyrus L. growing in central and northern Iraq. The Journal of Tikrit University of Agricultural Sciences - A special issue of the Third Specialized Conference, pp. 274-282. (in Arabic)
Tingey, S. V. and Tufo Del, J. P. (1993). Genetic analysis with RAPDmarkers. Plant physiology. 101: 349- 352 pp
Nizam, Mohamed Qais (2014) studying genetic diversity within the wild peach species in Latakia Governorate. Master's thesis. Faculty of Agriculture. Tishreen University. Syria. (in Arabic)
Al-Qaisi, Imad Khalaf Khader. (2013) Estimate of genetic action in some field traits in Zea mays. Doctoral thesis, Faculty of Agriculture and Forestry, University of Mosul. (in Arabic)
Yadav, J. P.; Kumar, S.; Yadav, M.; Kadyan, S.; and Yadav, S. (2014). Assesment of genetic diversity using RAPD marker among different accessions of Salvadora Oleoides of North- west India. Bioresearch Bulletin, Volume 4.1.
Bai D., Brandle J. and Reeleder R.(1997). Genetic diversity in north American ginseng (Paxa quinquefolius L.) Grown in Ontario by RAPD analysis . Genome , 40:111 -115
Penner G.A., Clarke J.,Bezta L.J.and Leisle D.(1995).Identification of RAPD markers linked to a gene governing Candmium uptake in durum wheat .Genome , 38:543 – 547.
Al-Karkhi, Hadeel Abdullah Hatem, Jassim Mohamed Aziz Al-Jubouri, Nowruz Abdul Razzaq Taher. (2018). Estimating the genetic dimension of several genetic structures of baking wheat aestivum triticum L. based on RAPD technology. Tikrit University of Agricultural Sciences Journal. 18 (1) p. 49 - 66. (in Arabic)
Williams, C. E. and St- Clair, D. A. (1993). Phonetic relationships and Level of variability detected by restriction fragment length polymorphism and random amplified DNA analysis of cultivated and wild accessions of Lycopersican esculentum Genome. 36: 619-930
Warburton M.L., Bliss F.A. (1996) Genetic Diversity in Peach (Prunus persica L. Batch) Revealed by Randomly Amplified Polymorphic DNA (RAPD) Markers and Compared to Inbreeding Coefficients . J. AMER. SOC. HORT. SCI .121(6). 1012–1019.
Mir, J. I.; Ahmed, N.; Rashid, R.; Wan, S. H.; Sheikh, M.A.; Mir, H.; Parveen, I.; Shah, S. (2012)Genetic diversity analysis in apricot (Prunus armeniaca L.) germplasm using RAPD markers. Indian Jornal of Biotechnology, 11, 187-190
Martínez-Mora C., J. Rodríguez, J. L. Cenis and L. Ruiz-García (2009) Genetic variability among local apricots (Prunus armeniaca L.)from the Southeast of Spain. Spanish Journal of Agricultural Research . 7(4), 855-868
Lisek, A.; Korbin, M.; Rozpara, E(2006) Using simply generated RAPD markers to distinguish between sweet cherry (Prunus avium L.) cultivars. Journal of Fruit and Ornamental Plant Research, 14 , 2006, 51-58
Ait bella Y, Bouda S, Haddioui A (2018) Phenotypic Diversity Analysis of Plum (Prunus domestica L.) Cultivars. Phytomorphology. 68 (3&4): 93-101
Aryani, L. N. A., & Lesmana, C. B. J. (2019). Neuropsychiatric factor and polymorphism gene in internet addiction. International Journal of Health & Medical Sciences, 2(1), 39-44. https://doi.org/10.31295/ijhms.v2n1.90
Widana, I.K., Sumetri, N.W., Sutapa, I.K., Suryasa, W. (2021). Anthropometric measures for better cardiovascular and musculoskeletal health. Computer Applications in Engineering Education, 29(3), 550–561. https://doi.org/10.1002/cae.22202
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