Antibiotics susceptibility against Pseudomonas aeruginosa isolated from different infections

https://doi.org/10.53730/ijhs.v6nS5.9099

Authors

  • Donya, M. Mubdir Maternity and Children's Hospital, Ministry of Health, Iraq
  • Ameem N. Oda College of pharmacy, Al-Ahliyya Amman University, Jordan
  • Dunya N. Oda College of pharmacy, Al-Ahliyya Amman University, Jordan
  • Ilham A. Bunyan Dept. of Microbiology, College of Medicine, University of Babylon, Iraq

Keywords:

Pseudomonas aeruginosa, antibiotics susceptibility, Compact VITEK-2 System, UTIs, wound infection

Abstract

In the present study, a total 70 samples were collected from different sites of infection which include 25(35.7%) samples from bronchial wash, 15 (21.4%) samples from urine, 15 (21.4%) samples from ear swabs, and 15 (21.4%) samples wound swabs), these samples have been collected and tested during period from March 2022 to June 2022. The results showed that, out of 70 samples, 62(88.5%) give positive culture, while 8(11.5%) samples were negative culture. Out of 62 positive culture on different types of growth media, and the bacterial was identified according to gram stain, biochemical tests and Vitek compact system, the results showed that, only 18(29.1%) isolates were related to Pseudomonas aeruginosa, while 44 (70.9%) samples were related to other types of microbial agents, these isolates which include 5/18(28%) isolates from bronchial wash, 4/18(22.1%) isolates from urine, 2/18(11.1%) isolates from ear swabs and 7/18(38.8%) isolates from wound swabs. To confirm the isolates of bacteria was used automated Compact Vitek-2 system use GN-ID cards which contained 64 biochemical tests. 

Downloads

Download data is not yet available.

References

Basu, S., Copana, R., Morales, R., Anugulruengkitt, S., Puthanakit, T., Maramba-Lazarte, C., ... & Bryant, P. A. (2022). Keeping it real: antibiotic use problems and stewardship solutions in low-and middle-income countries. The Pediatric Infectious Disease Journal, 41(3), S18-S25.‏

Coates, A. R., Hu, Y., Holt, J., & Yeh, P. (2020). Antibiotic combination therapy against resistant bacterial infections: synergy, rejuvenation and resistance reduction. Expert review of Anti-infective therapy, 18(1), 5-15.‏

Ryan, M. P., & Pembroke, J. T. (2018). Brevundimonas spp: emerging global opportunistic pathogens. Virulence, 9(1), 480-493.‏

Arirachakaran, P., Luangworakhun, S., Charalampakis, G., & Dahlén, G. (2019). Non‐oral, aerobic, Gram‐negative bacilli in the oral cavity of Thai HIV‐positive patients on Highly‐active anti‐retrovirus therapy medication. Journal of Investigative and Clinical Dentistry, 10(2), e12387.‏

David, S. M., Jayaprakash, C., & Mathew, A. (2020). Isolation, Identification and Antibiotic Susceptibility Testing of Pseudomonas aeruginosa and Acinetobacter baumannii from Endotracheal Secretions in a Tertiary Care Centre. Int. J. Curr. Microbiol. App. Sci, 9(2), 1566-1574.‏

Schito, A. M., & Alfei, S. (2020). Antibacterial activity of non-cytotoxic, amino acid-modified polycationic dendrimers against Pseudomonas aeruginosa and other non-fermenting gram-negative bacteria. Polymers, 12(8), 1818.‏

Mustaqueem, M., Mahto, V., Poddar, C. K., Chouhan, R., Kumar, P. M., & Singh, M. N. (2021). Path-Organism Burden and Antibiogram Outline of Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital of Jamshedpur, Jharkhand, India. morbidity and mortality, 8, 9.‏

Yadav, S. K., Bhujel, R., Mishra, S. K., Sharma, S., & Sherchand, J. B. (2020). Emergence of multidrug-resistant non-fermentative gram negative bacterial infection in hospitalized patients in a tertiary care center of Nepal. BMC research notes, 13(1), 1-6.‏

Behzadi, P., Baráth, Z., & Gajdács, M. (2021). It’s not easy being green: a narrative review on the microbiology, virulence and therapeutic prospects of multidrug-resistant Pseudomonas aeruginosa. Antibiotics, 10(1), 42.‏

Mohamed, A., & Abdelhamid, F. (2020). Antibiotic susceptibility of Pseudomonas aeruginosa isolated from different clinical sources. Zagazig Journal of Pharmaceutical Sciences, 28(2), 10-17.‏

Spencer, H. K., Spitznogle, S. L., Borjan, J., & Aitken, S. L. (2020). An Overview of the Treatment of Less Common Non–Lactose‐Fermenting Gram‐Negative Bacteria. Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, 40(9), 936-951.‏

Rahman, M. A., & Nair, P. O. O. J. A. (2021). PREVALENCE AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS SPECIES ISOLATED FROM CLINICAL SAMPLES IN A TERTIARYCAREHOSPITAL. International Journal of Current Pharmaceutical Research, 13(1), 50-3.‏

de Freitas, P. M., Feitosa, R. J. P., Couto, M. P., dos Santos Pereira, H., Catão, R. M. R., & Santos Filho, L. (2018). Of nosocomial infections by gram-negative non-fermenters and profile of antimicrobial sensitivity in a hospital in Campina Grande-PB. Journal of Biology & Pharmacy and Agricultural Management, 14(2).‏

Pirzadian, J., Harteveld, S. P., Ramdutt, S. N., van Wamel, W. J., Klaassen, C. H., Vos, M. C., & Severin, J. A. (2020). Novel use of culturomics to identify the microbiota in hospital sink drains with and without persistent VIM-positive Pseudomonas aeruginosa. Scientific reports, 10(1), 1-12.‏

McFadden, J. F. (2000) .Biochemical tests for Identification of Medical Bacteria 3rd Ed. The Williams & Wilkins Co., USA. PP: 689 – 691.

Collee, J. G., Fraser, A. G., Marmion, B. P. and Simmons, A. (1996). Mackie and Mecartney. Practical Medical Microbiology. 14thEd. Churchill Living stone, USA. 413 – 424.

Clinical and laboratory standards institute (CLSI). (2019): informational supplement. For slandered antimicrobial susceptibility testing. Assorted slandered. 30(1): 100-s20.

Das, T. (2021). Introductory Chapter: Understanding Infections Caused by Opportunistic Bacterial Pathogens. In Pseudomonas aeruginosa-Biofilm Formation, Infections and Treatments. IntechOpen.‏

Saleh, M. M., Abbas, H. A., & Askoura, M. M. (2019). Repositioning secnidazole as a novel virulence factors attenuating agent in Pseudomonas aeruginosa. Microbial pathogenesis, 127, 31-38.‏

Kwon, K. T., & Armstrong, D. G. (2018). Microbiology and antimicrobial therapy for diabetic foot infections. Infection & chemotherapy, 50(1), 11-20.‏

Theuretzbacher, U., & Piddock, L. J. (2019). Non-traditional antibacterial therapeutic options and challenges. Cell host & microbe, 26(1), 61-72.‏

Pachori, P., Gothalwal, R., & Gandhi, P. (2019). Emergence of antibiotic resistance Pseudomonas aeruginosa in intensive care unit; a critical review. Genes & diseases, 6(2), 109-119.‏

Motbainor, H., Bereded, F., & Mulu, W. (2020). Multi-drug resistance of blood stream, urinary tract and surgical site nosocomial infections of Acinetobacter baumannii and Pseudomonas aeruginosa among patients hospitalized at Felegehiwot referral hospital, Northwest Ethiopia: a cross-sectional study. BMC infectious diseases, 20(1), 1-11.‏

Steelman, V. M., Shaw, C., Shine, L., & Hardy-Fairbanks, A. J. (2019). Unintentionally retained foreign objects: a descriptive study of 308 sentinel events and contributing factors. The Joint Commission Journal on Quality and Patient Safety, 45(4), 249-258.‏

Tong, Y., Zhang, J., Fu, Y., He, X., & Feng, Q. (2022). Risk Factors and Outcome of Sepsis in Traumatic Patients and Pathogen Detection Using Metagenomic Next-Generation Sequencing. Canadian Journal of Infectious Diseases and Medical Microbiology, 2022.‏

Samrot, A. V., Raji, P., Selvarani, A. J., & Nishanthini, P. (2018). Antibacterial activity of some edible fruits and its green synthesized silver nanoparticles against uropathogen–Pseudomonas aeruginosa SU 18. Biocatalysis and agricultural biotechnology, 16, 253-270.‏

Mohamed, A., & Abdelhamid, F. (2020). Antibiotic susceptibility of Pseudomonas aeruginosa isolated from different clinical sources. Zagazig Journal of Pharmaceutical Sciences, 28(2), 10-17.‏

Olivares, E., Badel-Berchoux, S., Provot, C., Prévost, G., Bernardi, T., & Jehl, F. (2020). Clinical impact of antibiotics for the treatment of Pseudomonas aeruginosa biofilm infections. Frontiers in microbiology, 10, 2894.‏

Walker, G. T., Quan, J., Higgins, S. G., Toraskar, N., Chang, W., Saeed, A., ... & Sahm, D. (2019). Predicting antibiotic resistance in gram-negative bacilli from resistance genes. Antimicrobial Agents and Chemotherapy, 63(4), e02462-18.‏

Talebi Bezmin Abadi, A., Rizvanov, A. A., Haertlé, T., & Blatt, N. L. (2019). World Health Organization report: current crisis of antibiotic resistance. BioNanoScience, 9(4), 778-788.‏

Fahim, N. A. E. (2021). Prevalence and antimicrobial susceptibility profile of multidrug-resistant bacteria among intensive care units patients at Ain Shams University Hospitals in Egypt—a retrospective study. Journal of the Egyptian Public Health Association, 96(1), 1-10.‏

Bunyan, I. A., Hadi, O. M., & Al-Mansoori, H. A. (2018). Molecular detection of Metallo-beta lactamase producing Pseudomonas aeruginosa isolated from different sites of infection. Journal of Pharmaceutical Sciences and Research, 10(5), 1072-1078.‏

Bunyan, I. A., Naji, S. S., & Aljodoa, H. H. (2018). Molecular study of adhesive properties in some bacteria isolated from throat infections. Biochem. Cell. Arch, 18, 2013-2021.‏

Published

17-06-2022

How to Cite

Donya, M. M., Oda, A. N., Oda, D. N., & Bunyan, I. A. (2022). Antibiotics susceptibility against Pseudomonas aeruginosa isolated from different infections. International Journal of Health Sciences, 6(S5), 1148–1156. https://doi.org/10.53730/ijhs.v6nS5.9099

Issue

Section

Peer Review Articles