Characterization and in-vitro cytotoxicity of lupeol isolated from leaf extract of ficus mysorensis

Deepti Palleda; V Krishna; Venkatesh Ramu

doi:10.53730/ijhs.v6nS5.9105

Authors

Deepti Palleda P.G. Department of Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta 577 451, Karnataka, India
Krishna V
krishnabiotech2003@gmail.com
P.G. Department of Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta 577 451, Karnataka, India
Venkatesh Ramu Department of Studies in Food Technology, Davangere University, Shivagangotri campus, Davanagere - 577507

Keywords:

Ficus mysorensis, in-vitro cytotoxicity, Lupeol, MCF-7

Abstract

Medicinal plant extracts gain more attention in research of modern medical sciences due to their non-lethal activity. The use of traditional medicine practices from plants has been accepted as a main sources for drug discovery in various health disorder especially cancer. Ficus mysorensis has a long history of usage as traditional medicine. The leaf extract of this plant possess phytochemicals such as alkaloids, flavonoids, terpenoids, steroids, saponins, Phenolics and glycosides. The ethanol leaf extract has been subjected to compound isolation and confirmed by GCMS, HPLC Chromatogram, ¹³C and ¹H nuclear magnetic resonance (NMR), IR spectra. Based on spectral studies the isolated compound confirmed that Lupeol. Lupeol compound was evaluated against MCF-7 cell lines by MTT assay. Lupeol induced an effective change in the cell viability of MCF-7 cells with IC50 concentration (198.75 g/ml). Induction of cell death, change in cell morphology and cancerous cells population was observed in the treated cells, but the normal cells was not affected.

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References

Akter, R., Gealt, M. A., Kleve, M. G., & Hossain, M. D. Z. (2016). Cytotoxicity of wortmannin triggers programmed cell death in MCF-7 cells; biochemical and morphological analysis. J Cancer Prev Curr Res, 4(4), 00125.

Aruoma, O. I., Bahorun, T., & Jen, L. S. (2003). Neuroprotection by bioactive components in medicinal and food plant extracts. Mutation Research/Reviews in Mutation Research, 544(2-3), 203-215.

Bafor, E. E., Lim, C. V., Rowan, E. G., & Edrada-Ebel, R. (2013). The leaves of Ficus exasperata Vahl (Moraceae) generates uterine active chemical constituents. Journal of Ethnopharmacology, 145(3), 803-812.

Cherkaoui, A., Hibbs, J., Emonet, S., Tangomo, M., Girard, M., Francois, P., & Schrenzel, J. (2010). Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. Journal of clinical microbiology, 48(4), 1169-1175.

Christophoridou, S., Dais, P., Tseng, L. H., & Spraul, M. (2005). Separation and identification of phenolic compounds in olive oil by coupling high-performance liquid chromatography with postcolumn solid-phase extraction to nuclear magnetic resonance spectroscopy (LC-SPE-NMR). Journal of Agricultural and Food Chemistry, 53(12), 4667-4679.

Colegate, S. M., & Molyneux, R. J. (Eds.). (2007). Bioactive natural products: detection, isolation, and structural determination. CRC press.

Cragg, G. M., Newman, D. J., & Snader, K. M. (1997). Natural products in drug discovery and development. Journal of natural products, 60(1), 52-60.

Dubey, N. K., Kumar, R., & Tripathi, P. (2004). Global promotion of herbal medicine: India's opportunity. Current science, 86(1), 37-41.

El-Olemy, M. M., Al-Muhtadi, F. J., & Afifi, A. F. A. (1994). Experimental phytochemistry: A laboratory manual. King Saud University Press.

Ferreira, J. R. O., Cavalcanti, B. C., da Costa, P. M., de Arantes, F. F. P., de Alvarenga, E. S., Maltha, C. R. A., & Ferreira, P. M. P. (2013). Induction of G2/M arrest, caspase activation and apoptosis by α-santonin derivatives in HL-60 cells. Toxicology in Vitro, 27(5), 1458-1466.

Gerlier, D., & Thomasset, N. (1986). Use of MTT colorimetric assay to measure cell activation. Journal of immunological methods, 94(1-2), 57-63.

Gokhale, A. B., Damre, A. S., & Saraf, M. N. (2003). Investigations into the immunomodulatory activity of Argyreia speciosa. Journal of ethnopharmacology, 84(1), 109-114.

Gullo, V. P. (Ed.). (2013). Discovery of novel natural products with therapeutic potential (Vol. 26). Newnes.

Harborne, A. J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media.

Iman, S., Azhar, I., Hasan, M. M., Ali, M. S., & Ahwed, S. W. (2007). Two triterpenes lupanone and lupeol isolated and identified from Tamarindus indica L. Pak J Pharm Sci, 20, 125-7.

Jin, S. E., Son, Y. K., Min, B. S., Jung, H. A., & Choi, J. S. (2012). Anti-inflammatory and antioxidant activities of constituents isolated from Pueraria lobata roots. Archives of Pharmacal Research, 35(5), 823-837.

Kalegari, M., Miguel, M. D., Dias, J. D. F. G., Lordello, A. L. L., Lima, C. P. D., Miyazaki, C. M. S., & Miguel, O. G. (2011). Phytochemical constituents and preliminary toxicity evaluation of leaves from Rourea induta Planch.(Connaraceae). Brazilian Journal of Pharmaceutical Sciences, 47(3), 635-642.

Kemp, W. (1991). Infrared spectroscopy. In Organic Spectroscopy (pp. 19-99). Palgrave, London.

Kinghorn, A. D., & Balandrin, M. F. (Eds.). (1993). Human medicinal agents from plants. American Chemical Society.

Kunwar, R. M., & Bussmann, R. W. (2006). Ficus (Fig) species in Nepal: a review of diversity and indigenous uses. Lyonia, 11(1), 85-97.

Lambertini, E., Lampronti, I., Penolazzi, L., Khan, M. T. H., Ather, A., Giorgi, G., & Piva, R. (2005). Expression of estrogen receptor α gene in breast cancer cells treated with transcription factor decoy is modulated by Bangladeshi natural plant extracts. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 15(2), 69-79.

Laszczyk, M. N. (2009). Pentacyclic triterpenes of the lupane, oleanane and ursane group as tools in cancer therapy. Planta medica, 75(15), 1549-1560.

Lee, C. K., & Chang, M. H. (2000). The chemical constituents from the heartwood of Eucalyptus citriodora. Journal of the Chinese Chemical Society, 47(3), 555-560.

Lee, T. K. W., Castilho, A., Cheung, V. C. H., Tang, K. H., Ma, S., & Ng, I. O. L. (2011). Lupeol targets liver tumor‐initiating cells through phosphatase and tensin homolog modulation. Hepatology, 53(1), 160-170.

Liby, K. T., Yore, M. M., & Sporn, M. B. (2007). Triterpenoids and rexinoids as multifunctional agents for the prevention and treatment of cancer. Nature Reviews Cancer, 7(5), 357-369.

Lilly V. (2011) Herbal lupeol as potent antidiabetic active principle on Sterptozotoin induced diabetic Wistar rats. 2011. p. 227. Available from: http://www.hdl.handle.net/10603/4775 .

Liu, F., He, Y., Liang, Y., Wen, L., Zhu, Y., Wu, Y., ... & Liu, H. (2013). PI3-kinase inhibition synergistically promoted the anti-tumor effect of lupeol in hepatocellular carcinoma. Cancer Cell International, 13(1), 1-7.

Morris, K. T., Johnson, N., Homer, L., & Walts, D. (2000). A comparison of complementary therapy use between breast cancer patients and patients with other primary tumor sites. The American Journal of Surgery, 179(5), 407-411.

Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63.

Mukherjee, P. K., Nema, N. K., Bhadra, S., Mukherjee, D., Braga, F. C., & Matsabisa, M. G. (2014). Immunomodulatory leads from medicinal plants. Indian Journal of Traditional Knowledge, 13, 235-256.

Nascimento, M., Arruda, A. C., Arruda, M. S. P., Müler, A. H., & Yoshioka, C. Y. (1999). Aripuanin, a megastigmane skeleton from Ficus aripuanensis. Fitoterapia, 70(6), 628-629.

Newman, V., Rock, C. L., Faerber, S., Flatt, S. W., Wright, F. A., Pierce, J. P., & LIVING STUDY GROUP. (1998). Dietary supplement use by women at risk for breast cancer recurrence. Journal of the American Dietetic Association, 98(3), 285-292.

Pandey, M. K., Sung, B., Ahn, K. S., Kunnumakkara, A. B., Chaturvedi, M. M., & Aggarwal, B. B. (2007). Gambogic acid, a novel ligand for transferrin receptor, potentiates TNF-induced apoptosis through modulation of the nuclear factor-κB signaling pathway. Blood, The Journal of the American Society of Hematology, 110(10), 3517-3525.

Popova, I. E., Hall, C., & Kubátová, A. (2009). Determination of lignans in flaxseed using liquid chromatography with time-of-flight mass spectrometry. Journal of Chromatography A, 1216(2), 217-229.

Ratnasooriya, W. D., Jayakody, J. R. A. C., & Dharmasiri, M. G. (1998). An aqueous extract of trunk bark of Ficus religiosa has anxiolytic activity. Medical science research, 26, 817-819.

Saleem, M., Afaq, F., Adhami, V. M., & Mukhtar, H. (2004). Lupeol modulates NF-κB and PI3K/Akt pathways and inhibits skin cancer in CD-1 mice. Oncogene, 23(30), 5203-5214.

Setzer, W. N., & Setzer, M. C. (2003). Plant-derived triterpenoids as potential antineoplastic agents. Mini reviews in medicinal chemistry, 3(6), 540-556.

Siddique, H. R., Mishra, S. K., Karnes, R. J., & Saleem, M. (2011). Lupeol, a novel androgen receptor inhibitor: implications in prostate cancer therapy. Clinical Cancer Research, 17(16), 5379-5391.

Tarapore, R. S., Siddiqui, I. A., Adhami, V. M., Spiegelman, V. S., & Mukhtar, H. (2013). The dietary terpene lupeol targets colorectal cancer cells with constitutively active Wnt/β‐catenin signaling. Molecular nutrition & food research, 57(11), 1950-1958.

Vinutha, B., Prashanth, D., Salma, K., Sreeja, S. L., Pratiti, D., Padmaja, R., & Deepak, M. (2007). Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity. Journal of ethnopharmacology, 109(2), 359-363.

Vyawahare, N. S., Khandelwal, A. R., Batra, V. R., & Nikam, A. P. (2007). Herbal anticonvulsants. J Herbal Med Toxicol, 1(1), 9-14.

Wagner, W. L., Herbst, D. R., & Sohmer, S. H. (1999). Manual of the Flowering Plants of Hawai'i, Vols. 1 and 2 (No. Edn 2). University of Hawai'i and Bishop Museum Press.

Wu, X. T., Liu, J. Q., Lu, X. T., Chen, F. X., Zhou, Z. H., Wang, T., & Fei, S. J. (2013). The enhanced effect of lupeol on the destruction of gastric cancer cells by NK cells. International Immunopharmacology, 16(2), 332-340.

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949