The efficacy of Carica papaya leaf extract against some bacteria which isolated from cow milk
Keywords:
Bacterial mastitis, Papaya extracts, GC mass of papaya leafAbstract
The advent of science to the search for antibiotics principally depends on medicinal plants as raw materials. Pathogenic bacteria isolated from cow milk represent play a major role in mastitis and are not easy to treat even with the use of a wide range of antibacterial agents. Finding alternative substances that may eliminate those bacteria with fewer side effects especially if they are derived from natural sources. Here, papaya leaf was collected, air-dried, powdered and extracts (PLE) of tow solvents; ethanol (EPLE) and aqueous (EPLA) were used to evaluate their Mueller-Hinton based inhibition activities against Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus (isolated from milk of local cows in the current work). The results showed that the ethanol extract of papaya leaves (EPLE) was highly effective in decreasing the growth of Klebsiella pneumoniae and Staphylococcus aureus, while did not show any activity in inhibiting of E. coli. Compared with the aqueous extract of papaya leaves (EPLA), which did not record any inhibition of the growth of bacteria isolated for the study only in its high concentration (80%) for each (E. coli and Klebsiella pneumoniae).
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Abebe R, Hatiya H, Abera M, and Megersa B, Asmare K. Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hiwassee milk shed, South Ethiopia. BMC Vet Res [Internet]. 2016 Dec 3 [cited 2020 Apr 29]; 12(1):270.
Keefe G. Update on control of staphylococcus aureus and streptococcus agalactiae for management of mastitis. Vol. 28, Veterinary Clinics of North America - Food Animal Practice. 2012. p. 203–16.
Nielsen C, Ostergaard S, Emanuel’s son U, Andersson H, Berglund B, Strindberg E. Economic consequences of mastitis and withdrawal of milk with high somatic cell count in Swedish dairy herds. Animal. 2010 May; 4(10):1758–70.
Pereira UP, Oliveira DGS, Mesquite LR, Costa GM, Pereira LJ. Efficacy of Staphylococcus aureus vaccines for bovine mastitis: A systematic review. Vol. 148, Veterinary Microbiology. 2011. p. 117–24.
Pérez MM, Prenafeta A, Valle J, Penadés J, Rota C, Solano C, et al. Protection from Staphylococcus aureus mastitis associated with poly-N-acetyl β-1,6 glucosamine specific antibody production using biofilm embedded bacteria. Vaccine. 2009 Apr 14; 27(17):2379–86.
Barkema HW, Schukken YH, Zadoks RN. Invited review: The role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis [Internet]. Vol. 89, Journal of Dairy Science. American Dairy Science Association; 2006 [cited 2020 Apr 29]. p. 1877–95.
Schukken YH, Bronzo V, Locatelli C, Pollera C, Rota N, Casula A, et al. Efficacy of vaccination on Staphylococcus aureus and coagulase-negative staphylococci intermammary infection dynamics in 2 dairy herds. J Dairy Sci [Internet]. 2014 [cited 2020 Apr 29]; 97(8):5250–64.
Persson Y, Nyman AKJ, Ground-Andersson U. Etiology and antimicrobial susceptibility of udder pathogens from cases of subclinical mastitis in dairy cows in Sweden. Acta Vet Scand [Internet]. 2011 Jun 8 [cited 2020 Apr 29];53(1):36. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21649936
Ericsson Understudy H, Lindberg A, Persson Waller K, Ekman T, Artursson K, Nilsson-Cost M, et al. Microbial etiology of acute clinical mastitis and agent-specific risk factors. Vet Microbiol. 2009 May 28;137(1–2):90–7.
Hogeveen H, Huijps K, Lam TJGM. Economic aspects of mastitis: New developments [Internet]. Vol. 59, New Zealand Veterinary Journal. 2011 [cited 2020 Apr 29]. p. 16–23.
A. Keba, M. L. Rolon, A. Tamene et al., “Review of the prevalence of foodborne pathogens in milk and dairy products in Ethiopia,” International Dairy Journal, vol. 109, Article ID 104762, 2020.
Ali KhoddamiORCID,Meredith A. Wilkes andThomas H. Roberts.2013 Techniques for Analysis of Plant phenolic compounds.
Oliver SP, Murinda SE. Antimicrobial resistance of mastitis pathogens. Vol. 28, Veterinary Clinics of North America - Food Animal Practice. 2012. p. 165–85.
Gao J, Barkema HW, Zhang L, Liu G, Deng Z, Cai L, et al. Incidence of clinical mastitis and distribution of pathogens on large Chinese dairy farms. J Dairy Sci. 2017 Jun 1; 100(6):4797–806.
Abebe R, Hatiya H, Abera M, Megersa B, Asmare K. Bovine mastitis: Prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Vet Res. 2016 Dec 3; 12(1).
Cheng J, Qu W, Barkema HW, Nobrega DB, Gao J, Liu G, et al. antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds. J Dairy Sci. 2019 Mar 1; 102(3):2416–26.
3-Saran, P. L.; Solanki, I. S. and Choudhary (2016). Papaya Biology, cultivation Production and Uses. CRC Press, Taylor & Francis Group, New York, 266.
Radha krishnan, N.; Lam, K. W and Norhaizan, M. E. (2017). Molecular docking analysis of Carica papaya Linn constituents as antiviral agent. International Food Research Journal, 24(4): 1819-1825.
Mohammed, M.A., Abdulridha, W.M., Abdi, A.N., (2018), Thickness effect on some physical properties of the Ag thin films prepared by thermal evaporation technique, Journal of Global Pharma Technology this, 10(3), pp. 613– 619.
Abdull Razis, A. F.; Ibrahim, M. D. and Kntayya, S. B. (2014). Health benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention, 15(20): 8571–8576.
Suresh, L., R.M. Veerabah and S.R. Gnanasingh, (2010). GC-MS analysis of ethanolic extract of Zanthoxylum rhetsa (roxb.) dc spines. Journal Herbal Med. Toxicol., 4: 191-192.
Omojasola, P. F. and Awe, S. 2004. The Antibacterial activity of the leaf extract of Anacardium occidentale and Gossyptum hirsutum against some selected microorganisms. Bioscience Research Communication 16 (1).
Al-Bayati, F. A. and Sulaiman, K. D., 2008. In Vitro Antimicrobial activity of Salvadorapersica L. Extracts against Some isolated Oral pathogens in Iraq. Turk. J. Biol 57-62.
Ajani, E. O., Bamisaye, F. A. and Minari J. B., 2013. Prospects of Ethnobotanical Uses of Pawpaw (Carica papaya) J. Med. Plants Studies, 1 (4)
Doughari, J. H., 2006. Antimicrobial activity of Tamarindusindica Linn. Tropical J. Pharm. Res. 5(2): 592- 603
Nwofia, G. E., Ogimelukwe, P. and Eji, C., 2012. Chemical composition of leaves, fruit pulp and seed in some morph types of Carica papaya Leaf graphotypes. Int. J. Med. Arom. Plant 2:200- 206.
Omidiwura, B.R.O., 2017. Qualitative and quantitative analysis of pawpaw (Carica papaya) leaf extract and its antimicrobial effect in animal production. Nig. J. Anim. Prod., 44(3):78 – 83.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2022). Post-pandemic health and its sustainability: Educational situation. International Journal of Health Sciences, 6(1), i-v. https://doi.org/10.53730/ijhs.v6n1.5949
Suryasa, I.W., Sudipa, I.N., Puspani, I.A.M., Netra, I.M. (2019). Translation procedure of happy emotion of english into indonesian in kṛṣṇa text. Journal of Language Teaching and Research, 10(4), 738–746
Hepsiba, N., Subhashini, A., Raju, M., & Rao, Y. P. (2018). Changing role of teachers in the present society. International Journal of Health & Medical Sciences, 1(1), 35-38. https://doi.org/10.31295/ijhms.v1n1.37
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