Correction of the degree of endogenic intoxication and lipoproxidation processes by the infusion drug (rheoambrasol) with experimental hypoxia, induced by sodium nitrite

Jalol Djalolidinovic Khujakhmedov; Larisa Ivanovna Shevchenko; Khamid Yakubovich Karimov

doi:10.53730/ijhs.v6nS4.9143

Authors

Jalol Djalolidinovic Khujakhmedov Molecular genetics laboratory "GenoTekhnologiya", Tashkent, Uzbekistan
Larisa Ivanovna Shevchenko Department of Molecular Medicine and Cell Technologies, Republican Specialized Scientific-Practical Medical Center of Hematology, Tashkent, Uzbekistan
Khamid Yakubovich Karimov Department of Molecular Medicine and Cell Technologies, Republican Specialized Scientific-Practical Medical Center of Hematology, Tashkent, Uzbekistan

Keywords:

"Rheoambrasol", nitrite hypoxia, hypoxia-inducible factor (HIF-1α), endogenous intoxication, lipid peroxidation (LPO), antioxidant system (AOS)

Abstract

Rationale (relevance): Almost all known diseases, as a rule, are accompanied by a hypoxic state associated primarily with oxygen deficiency and impaired energy metabolism, which cause damage to the structure and functioning of organs and cells. Using a complex of a polysaccharide with a bioenergetic substrate, a blood-substituting infusion drug"Rheoambrasol" has been developed, which is able to protect cells from free radical oxidation and restore disturbed energy metabolism in cells during hypoxia. Purpose: to evaluate the effectiveness of correction of the hypoxia-inducible factor - HIF-1α, endogenous intoxication, the intensity of lipid peroxidation processes in the liver during experimental nitrite hypoxia with the new blood-substituting infusion drug "Rheoambrasol". Material and methods. The setting of the experimental hypoxia model was carried out in outbred male rats weighing 185 ± 10.2 g by administering sodium nitrite at a dose of 90 mg / kg. In the blood plasma of experimental animals, the content of hypoxia-inducible factor (HIF-1α), indicators of endogenous intoxication, the intensity of lipid peroxidation (LPO) and the activity of antioxidant enzymes systems (AOS) were determined in the liver.

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