Use molecular method to detect the screw worm fly in  Al-Diwaniyah province

Haneen Habeeb Noor Al-Hameedawi; Ghaidaa Abass Jasim

doi:10.53730/ijhs.v6nS4.9330

Authors

Haneen Habeeb Noor Al-Hameedawi
vet20.pst17@qu.edu.iq
University of Al-Qadisiyah, College of veterinary medicine
Ghaidaa Abass Jasim University of Al-Qadisiyah, College of veterinary medicine

Keywords:

Chrysomya bezziana, PCR, 16S rRNA gene

Abstract

Chrysomya bezziana this study was carried out between October, 2021 to March 31, 2022 in six region from Al-Diwaniyah province. Observe the influence of the month and type of animal on the region's. The total number of animals infected was 33 (1) cattle, (7) lambs, and (35) sheep. Al-muhnawa had the highest rate of infestation, while Al-hamza had the lowest rate of infestation. The highest rate of infestation recorded in March ()% and the lowest in February ()%.Conventional PCR (Polymerase Chain Reaction) was used for molecular identification. A DNA extraction was performed on 50 randomly chosen larvae from the total population. The size of the PCR product (548 pb) For Chrysomya bezziana, from the infected animals larvae were taken to DNA extracted and used following targeting 16S rRNA gene product size (548 bp). There follows sequence analysis to obtain the nucleotide (16S rRNA) gene, and the sequence was recoded in the National Center for Biotechnology (NCBI) with accession numbers (ON059979, ON059980, ON059981, ON059982, ON059983, ON059984, ON059985)Iraq. This gene's homology sequence identity percentage was calculated by comparing it to another globally registered gene (NCBI).

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