In vitro antilithiatic and antioxidant potential of ethanolic extract of bauhinia variegata leaves

Harshita Mishra; Kirti Rai; Albert Alex

doi:10.53730/ijhs.v6nS5.9354

Authors

Harshita Mishra
harshi9.hm@gmail.com
Research Scholar, HIMT College of Pharmacy, Greater Noida, Uttar Pradesh, India
Kirti Rai HIMT College of Pharmacy, Greater Noida, Uttar Pradesh, India
Albert Alex HIMT College of Pharmacy, Greater Noida, Uttar Pradesh, India.

Keywords:

Antilithiatic activity, Bauhinia variegate, Kidney stone, antioxidant, Calcium Oxalate

Abstract

Calcium oxalate crystals are responsible for formation of urinary calculi. Traditional therapy based on plants and herbal resources have found beneficial for treatment of kidney stones. The present study aimed to screen antilithiatic activity and antioxidant potential of ethanolic extract of Bauhinia variegate leaves. The ethanolic extract of leaves was obtained through continuous heat extraction process and evaluated for the presence of secondary metabolites, total phenolic content and total flavonoid contents. The high phenolic content in leaves is attributable to the presence of phenol, flavonoid, and tannin which all have antioxidant properties, and same was evaluated and confirmed using nitric oxide scavenging method and assessing reducing power of extract. Antilithiatic potential of few secondary metabolites like triterpenoids was confirmed by evaluation calcium oxalate dissolving ability of plant’s ethanolic extract. The urinary calculi dissolving ability was of extract was assessed in vitro. The overall findings confirmed the antilithiatic and antioxidant potential of ethanolic extract of Bauhinia variegate Leaves.

Downloads

Download data is not yet available.

References

Dave, R; Nagani, K; Chanda S. 2010. Pharmacognostic studies and physicochemical properties of the Polyalthia longifolia var. pendula leaf. Pharmacognosy Journal, 2(13), 572-576.

Delfan, B; Baharvand-Ahmadi, B; Bahmani, et al. 2015. An ethnobotanical study of medicinal plants used in treatment of kidney stones and kidney pain in Lorestan province, Iran. Journal of Chemical and Pharmaceutical Sciences, 8(4), 693-699.

Deyong, Z.H.; Lei, W.A.; Yunting, L.E. 2012. Correlation among SDS sedimentation value, swelling index of glutenin and solvent retention capacity of spring wheat. Notulae Scientia Biologicae, 4(2), 132-135.

Ainsworth, E.A.; Gillespie, K.M. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols, 2(4), 875-877.

Atanassova, S; Neykov, K; Gutzow, I. 2000. Solubility and dissolution kinetics of calcium oxalate renal calculi in solutions, containing DL-lysine: in vitro experiments. Journal of crystal growth. 212(1-2), 233-238.

Bensatal, A; Ouahrani, M.R. 2008. Inhibition of crystallization of calcium oxalate by the extraction of Tamarix gallica L. Urological Research. 36(6), 283-287.

Chaudhary, A; Singla, S.K.; Tandon C. 2010. In vitro evaluation of Terminalia arjuna on calcium phosphate and calcium oxalate crystallization. Indian journal of pharmaceutical sciences. 72(3), 340.

Duh, P.D.; Yen, G.C.; Yen, W.J., et al. 2004. Effects of pu-erh tea on oxidative damage and nitric oxide scavenging. Journal of Agricultural and Food Chemistry. 52(26), 8169-8176.

Kachkoul, R.; Squalli, Housseini, T; Mohim, M; et al. 2019. Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones. J Integr Med, 17(6), 430–437.

Khan, S.R.; Pearle, M.S.; Robertson, W.G.; et al. 2016. Kidney stones. Nature reviews Disease primers. 2(1), 1-23.

L. Yachi, S. Bennis, Z. Aliat, A. Cheikh, et al. 2018. In vitro litholytic activity of some medicinal plants on urinary stones. African Journal of Urology, 24(3), 197-201.

Lin, JY; Tang, CY. 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food chemistry. 101(1),140-147.

Momin, RK; Kadam, VB. 2011. Determination of ash values of some medicinal plants of genus Sesbania of Marathwada region in Maharashtra. J Phytol. 3(12), 52-54.

Moore K, Roberts LJ. 1998. Measurement of lipid peroxidation. Free radical research. 28(6), 659-671.

Nielsen, SS. 2010. Determination of moisture content. In Food analysis laboratory manual, pp. 17-27. Springer, Boston, MA.

Nirmal, SA; Pal, SC; Mandal, SC; Patil AN. 2012. Analgesic and anti-inflammatory activity of β-sitosterol isolated from Nyctanthes arbortristis leaves. Inflammopharmacology, 20(4), 219-24.

Rinartha, K., Suryasa, W., & Kartika, L. G. S. (2018). Comparative Analysis of String Similarity on Dynamic Query Suggestions. In 2018 Electrical Power, Electronics, Communications, Controls and Informatics Seminar (EECCIS) (pp. 399-404). IEEE.

Roy, H; Chakraborty, A; Bhanja, S; et al. 2010. Preliminary phytochemical investigation and anthelmintic activity of Acanthospermum hispidum DC. Journal of Pharmaceutical Science and Technology. 2(5), 217-221.

Royer, DL. 2001. Stomatal density and stomatal index as indicators of paleoatmospheric CO2 concentration. Review of Palaeobotany and Palynology. 14(1-2), 1-28.

Scales, Jr CD; Smith, AC; Hanley, JM; Saigal CS. 2012. Urologic Diseases in America Project. Prevalence of kidney stones in the United States. European urology. 62(1), 160-165.

Sivarajan, VV; Balachandran, I. Ayurvedic drugs and their plant sources. Oxford and IBH publishing; 1994.

Sluiter, A; Ruiz, R; Scarlata, C. et al. 2005. Determination of extractives in biomass. Laboratory analytical procedure (LAP).1617, 1-9.

Xu, DL; Mo Y. 2011. Determination of foreign organic matter in Yemugua tablets by NIR correlation coefficient method. Chinese Journal of Pharmaceutical Analysis. 31(7), 1423-4.