Spectrophotometric determination of Mesalazine by a charge transfer complex

Abstract

A simple, developed, fast and accurate spectrophotometric method to determination Mesalazine (MES) in its pure form and pharmaceutical preparation (Pentasa). This method was based on the formation of a charge transfer complex between MES and the Iodine (IOD) reagent to give a purple color product that gives its highest absorption at the wavelength of 514 nm. The best conditions for complex formation were found (time, temperature, optimal reagent concentration and pH). The linearity of the method for the complex consisting ranged from 5-45 µg/ml, the Sandell's index was 0.03278 µg/cm², the molar absorption coefficient was 4670.6175  L/mol.cm and the detection limit was 0.10245 µg /mL, the quantitative limit was 0.31045  µg/ml, the percent recovery range was Rec% between (103.145 - 97.311 ) % and the relative standard deviation range RSD% between (0.128  -  0.263) %. It was found that the method is accurate , precision and has been successfully applied to determinate MES in its pharmaceutical preparation, in direct methods and in multi standard additions.