Morphological and molecular identification of Ascaridia galli isolated from local chicken (Gallus gallus domesticus) in Diayala Province, Iraq

Zainab Fadhil Rhaman; Amer Murhum Al-Amery

doi:10.53730/ijhs.v6nS4.9389

Authors

Zainab Fadhil Rhaman
zainab.khq90@gmail.com
Department of Parasitology, College of Veterinary Medicine, University of Diayala, Iraq
Amer Murhum Al-Amery Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq

Keywords:

Ascaridia spp, (18S rRNA) gene, phylogenetic tree, local chicken, Iraq

Abstract

The current study was conducted to investigate the prevalence of Ascaridia spp in local chickens from the beginning of October 2020 till the end of the March 2021 included examination of 120 local chickens (Gallus gallus domestica) in Diayala province. The total prevalence rate of Ascaridai spp. was 41.66%. Also, this study objective to detect A. spp. by the molecular diagnosis and detection the presence of 18SrRNA gene (724bp).The technique included genomic DNA extraction from adult worms isolated from small intestine of naturally infected local chickens with using tissue DNA extraction kits. This gene was amplified by using specific primers. Polymerase Chain Reaction (PCR) technique on the genomic DNA gave positive results at 724 bp . The randomly ten positive PCR products where sent for sequencing and phylogenetic analysis ,The sequences registered in NCBI gene bank under the accession numbers (MW732174.1), (MW732175.1), (MW732176.1), (MW732177.1), (MW732178.1), (MW732179.1), (MW732180.1), (MW732181.1), (MW732182.1), (MW732183.1) belong to Ascardia galli. S

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References

AL-jaumeili, A. H., & Aljoburi, A. U. J. M. (2015). The Parasites That Infect the Females for Domestic Fowl (Gallus Gallus Domesticus) in The Villages of The City of Sharqat, Salah Al-Deen Province. Tikrit Journal for Agricultural Sciences, 15(4):144-148.‏

Bharat, G. A., Kumar, N. P., Subhasish, B., & Ria, B. (2017). A report of Ascaridia galli in commercial poultry egg from India. J. World Poult. Res, 7: 23-26.‏

Bowman DD. 2009.Georgis’ Parasitology for Veterinarians.9th Ed. Westline industrial drive St. Louis, Missouri, p 142.

Eigaard, N. M., Schou, T. W., Permin, A., Christensen, J. P., Ekstrøm, C. T., Ambrosini, F., ... & Bisgaard, M. (2006). Infection and excretion of Salmonella enteritidis in two different chicken lines with concurrent Ascaridia galli infection. Avian Pathology, 35(6): 487-493.‏

Faraj, A. A., & Al-Amery, A. M. (2020). Microscopic and molecular diagnosis of Ascaridia spp. in domestic pigeons (Columba livia domestica) in baghdad city, iraq. The Iraqi Journal of Agricultural Science, 51(4): 734-743.‏

Kaingu F; Kibor B; Shivairo A.C(2010). Prevalence of gastrointestinal helminthes and coccidia in indigenous chicken from different agroclimatic zones in Kenya. African J Agri Res.5(6):458‒462.

Kassai T. 1999. Veterinary Helminthology, Butterworth-Heineman Publishing, Oxford, UK., p 222.

Okorie-Kanu, O. J., Ezenduka, E. V., Okorie-Kanu, C. O., Ugwu, L. C., & Nnamani, U. J. (2016). Occurrence and antimicrobial resistance of pathogenic Escherichia coli and Salmonella spp. in retail raw table eggs sold for human consumption in Enugu state, Nigeria. Veterinary world, 9(11): 1312-‏ 1319.

Radfar, M. H., Asl, E. N., Seghinsara, H. R., Dehaghi, M. M., & Fathi, S. (2012). Biodiversity and prevalence of parasites of domestic pigeons (Columba livia domestica) in a selected semiarid zone of South Khorasan, Iran. Tropical animal health and production, 44(2), 225-229.‏

Ramadan, H. H., & abouznada, N. Y. (1992). Morphology and life history of Ascaridia galli in the domestic fowl that are raised in Jeddah. Science, 4(1): 87-99.‏

Roussan, D. A., Shaheen, I. A., Khawaldeh, G. Y., Totanji, W. S., & Al-Rifai, R. H. (2012). Simultaneous detection of astrovirus, rotavirus, reovirus and adenovirus type I in broiler chicken flocks. Polish journal of veterinary sciences, 15(2) : 337-344.‏

Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: a laboratory manual (No. Ed. 2). Cold spring harbor laboratory press.‏

Sayyed R; Phulan M; Bhatti W(2000). Incidence of nematode parasites in commercial layers in swat. Pak Vet J. 20(2):107‒108.

Shthar, M. M. (2010). A comparative study of gastrointestinal helminthes infection between local and farm breeding houses in Baghdad province. Al-Anbar Journal of Veterinary Sciences, 3(1): 18-23.

Sivajothi, S., & Reddy, B. S. (2014). Lousicidal effect of deltamethrin in domestic pigeons. Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology, 40(3): 838-839.‏

Skallerup, P., Luna, L. A., Johansen, M. V., & Kyvsgaard, N. C. (2005). The impact of natural helminth infections and supplementary protein on growth performance of free-range chickens on smallholder farms in El Sauce, Nicaragua. Preventive veterinary medicine, 69(3-4): 229-244.‏

Soulsby, E. J. L. (1982). Helminths, arthropods and protozoa of domesticated animals (No. Ed. 7). Bailliere Tindall.‏

Tarbiat, B., Jansson, D. S., & Höglund, J. (2015). Environmental tolerance of free-living stages of the poultry roundworm Ascaridia galli. Veterinary parasitology, 209(1-2): 101-107.‏

Zada L; Rehman T, Niaz S(2015). Prevalence of Ascaridia galli in some poultry farms of district Mardan. J Adv Parasitol. 2(4):75‒79.

Zambrano, L. D., Levy, K., Menezes, N. P., & Freeman, M. C. (2014). Human diarrhea infections associated with domestic animal husbandry: a systematic review and meta-analysis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 108(6): 313-325.‏

Aryani, L. N. A., & Lesmana, C. B. J. (2019). Neuropsychiatric factor and polymorphism gene in internet addiction. International Journal of Health & Medical Sciences, 2(1), 39-44. https://doi.org/10.31295/ijhms.v2n1.90

Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864