Laboratory techniques for diagnosing Giardia parasite in dogs in Mosul City, Iraq
Keywords:
laboratory, diagnosing, parasiteAbstract
The results of the current study showed that the different techniques of microscopic examination recorded the highest rate of infection and diagnosis of Giardia in dogs with significant differences at the level of probability p<0.05 It reached 73.4%, followed by the rapid test technique and indirect enzyme linked immunosorbent assay (ELISA) with a rate of 14.2% and 12.2% respectively. With regard to determining the sensitivity and specificity of the rapid test and microscopic examination, a sensitivity of 19% and a specificity of 100% were recorded. The results, using the indirect ELISA showed an infection rate of 12.2% of the total samples. The results showed that the number of positive samples with this technique amounted to 12 samples positive for Giardia lamblia out of the total samples positive for microscopic examination. To confirm the diagnosis of giardiasis, the results using the direct immunofluorescence technique using acridine orange fluorochrome showed the appearance of a glowing brilliance, as the cysts appeared oval green glowing and the trophozoites appeared glowing green pear.
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References
Decock, C., Cadiergues, M. C., Larcher, M., Vermot, S., and Franc, M. (2003). Comparison of two techniques for diagnosis of giardiasis in dogs. Parasite, 10(1), 69-72.
Rajurkar, M. N., Lall, N., Basak, S., and Mallick, S. K. (2012). A simple method for demonstrating the Giardia lamblia trophozoite. Journal of Clinical and Diagnostic Research: JCDR, 6(9), 1492.
Ali, N, and Israa, A.W., 2017. Detection of Giardia infection in dogs of Basrah city., Basrah. Journal .Veterinary. Research,16(2):159-171.
Hassan, Z. I. (2021). Prevalence and Molecular Identification of Giardiasis Isolates from Stray Dogs in Erbil Province, Kurdistan Region, Iraq. Polytechnic Journal, 11(1), 7-12.
Symeonidou, I., Gelasakis, A. Ι., Miliotou, A. N., Angelou, A., Arsenopoulos, K. V., Loukeri, S., and Papadopoulos, E. (2020). Rapid on-site diagnosis of canine giardiosis: time versus performance. Parasites and Vectors, 13(1), 1-10.
Rahman, M. M., Hossain, M. A., Paul, S. K., Ahmed, S., Islam, A., Ehsan, M. A., and Sarkar, S. R. (2014). Detection of Giardia lamblia and Cryptosporidium parvum by direct immunofluorescence assay in stool specimen. Mymensingh Medical Journal: MMJ, 23(3), 426-429.
Uiterwijk, M., Nijsse, R., Kooyman, F. N., Wagenaar, J. A., Mughini-Gras, L., Koop, G., and Ploeger, H. W. (2018). Comparing four diagnostic tests for Giardia duodenalis in dogs using latent class analysis. Parasites and Vectors, 11(1), 1-8.
Uchôa, F. F. D. M., Sudré, A. P., Macieira, D. D. B., and Almosny, N. R. P. (2017). The influence of serial fecal sampling on the diagnosis of giardiasis in humans, dogs, and cats. Revista do Instituto de Medicina Tropical de São Paulo, 59.
Hooshyar, H., Rostamkhani, P., Arbabi, M., and Delavari, M. (2019). Giardia lamblia infection: review of current diagnostic strategies. Gastroenterology and Hepatology from Bed to Bench, 12(1), 3.
Olson, M. E., Leonard, N. J., and Strout, J. (2010). Prevalence and diagnosis of Giardia infection in dogs and cats using a fecal antigen test and fecal smear. The Canadian Veterinary Journal, 51(6), 640.
Alves, J., and Santos, A. (2016). Prevalence of Giardia spp. in young dogs using a combination of two diagnostic methods. Acta Parasitologica, 61(2), 261-266.
Alharbi, A., Toulah, F. H., Wakid, M. H., Azhar, E., Farraj, S., and Mirza, A. A. (2020). Detection of Giardia lamblia by microscopic examination, rapid chromatographic immunoassay test, and molecular technique. Cureus, 12(9).
Barbecho, J. M., Bowman, D. D., and Liotta, J. L. (2018). Comparative performance of reference laboratory tests and in-clinic tests for Giardia in canine feces. Parasites & vectors, 11(1), 1-4.
Saleh, M. N., Heptinstall, J. R., Johnson, E. M., Ballweber, L. R., Lindsay, D. S., Werre, S., and Zajac, A. M. (2019). Comparison of diagnostic techniques for detection of Giardia duodenalis in dogs and cats. Journal of Veterinary Internal Medicine, 33(3), 1272-1277.
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