Biosynthesis and antibacterial activity of Passiflora incarnata mediated copper oxide nanoparticles
Keywords:
passiflora incarnata, biosynthesis, antibacterial activity, listeria monocytogenesAbstract
Plant mediated nanoparticle synthesis is a rapidly growing field of study around the world. Plant biomolecules make non-toxic, stable, and cost-effective copper oxide nanoparticles In this study, eco-friendly CuONPs were prepared through a biosynthetic approach using Passiflora incarnata L. leaf extract. The synthesized CuONPs were characterized using UV-Visible spectroscopy (UV-Vis), scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Dynamic light scattering (DLS). Furthermore, the antibacterial activity was evaluated against Listeria monocytogenes. The SEM results indicated that rod-shaped CuONPs with an average particle size of 49.8 nm (size range of 39-63 nm) were synthesized and shown good antibacterial activity against Listeria monocytogenes. CuONPs produced from Passiflora incarnata leaf extract could be a potential antibacterial agent for treating foodborne infections caused by Listeria monocytogenes. However, more research is needed to determine how it works on the target microorganism.
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A.P. Angeline Mary a, A. T. A. b., ⇑, R. Subramanian c. (2019). Sugarcane juice mediated synthesis of copper oxide nanoparticles, characterization and their antibacterial activity. doi:10.1016/j.jksus.2019.03.003
Ahmad2, S. M. H. A. F. M. S. (2019). Terminalia belerica Mediated Green Synthesis of Nanoparticles of Copper, Iron and Zinc Metal Oxides as the Alternate Antibacterial Agents Against some Common Pathogens. doi:10.1007/s12668-019-0601-4
AKHTER, S. M. H. (2019). GREEN SYNTHESIS OF NANOMETALS USING MEDICINAL PLANT EXTRACT AND EVALUATION OF THEIR ANTIBACTERIAL EFFICACY.
Akl M. Awwad1, a. M. W. A. (2020). Biosynthesis of copper oxide nanoparticles using Ailanthus altissima leaf extract and antibacterial activity. doi:10.5281/zenodo.3670918
ARDEEP KUMAR1, A. G. N., SANDEEP PUNIA3, MANOJ KUMAR4, ZAHOOR ABBAS4, FALAK THAKRAL3, HARDEEP SINGH TULI3. (2019). SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF CUO NANOPARTICLES. doi:10.22159/ijap.2020v12i1.36271
Awwad, A. M., Albiss, B.A2, Salem N.M.3. (2015). Antibacterial Activity of synthesized Copper Oxide Nanoparticles using Malva sylvestris Leaf Extract.
Balazs, A. C., Emrick, T., & Russell, T. P. (2006). Nanoparticle polymer composites: where two small worlds meet. Science, 314(5802), 1107-1110. doi:10.1126/science.1130557
Baqer, A. A., Matori, K. A., Al-Hada, N. M., Kamari, H. M., Shaari, A. H., Saion, E., & Chyi, J. L. Y. J. J. o. M. S. M. i. E. (2018). Copper oxide nanoparticles synthesized by a heat treatment approach with structural, morphological and optical characteristics. 29(2), 1025-1033.
Bhakya, S., Muthukrishnan, S., Sukumaran, M., Muthukumar, M., Kumar, S. T., Rao, M. J. J. o. B., & Biodegredation. (2015). Catalytic degradation of organic dyes using synthesized silver nanoparticles: a green approach. 6(5), 1.
Bhavika Turakhia, M. B. D., Mysore Sridhar Santosh, Sejal Shah. (2020). Green synthesis of copper oxide nanoparticles: a promising approach in the development of antibacterial textiles. doi:10.1007/s11998-019-00303-5
Bradley, P. (1992). British herbal compendium. Volume 1. A handbook of scientific information on widely used plant drugs. Companion to Volume 1 of the British Herbal Pharmacopoeia.
Bruneton, J. (1995). Pharmacognosy, phytochemistry, medicinal plants Lavoisier Publishing Inc, c. In: o Springer Verlag, Secaucus, New Jersey, USA.
Chari, N., Felix, L., Davoodbasha, M., Sulaiman Ali, A., & Nooruddin, T. (2017). In vitro and in vivo antibiofilm effect of copper nanoparticles against aquaculture pathogens. Biocatalysis and Agricultural Biotechnology, 10, 336-341. doi:10.1016/j.bcab.2017.04.013
Chatzimitakos, T. G., & Stalikas, C. D. (2016). Qualitative Alterations of Bacterial Metabolome after Exposure to Metal Nanoparticles with Bactericidal Properties: A Comprehensive Workflow Based on (1)H NMR, UHPLC-HRMS, and Metabolic Databases. J Proteome Res, 15(9), 3322-3330. doi:10.1021/acs.jproteome.6b00489
Dodoo-Arhin, D., Leoni, M., & Scardi, P. (2012). Microemulsion Synthesis of Copper Oxide Nanorod-Like Structures. Molecular Crystals and Liquid Crystals, 555(1), 17-31. doi:10.1080/15421406.2012.634357
Dorner, L., Cancellieri, C., Rheingans, B., Walter, M., Kagi, R., Schmutz, P., . . . Jeurgens, L. P. H. (2019). Cost-effective sol-gel synthesis of porous CuO nanoparticle aggregates with tunable specific surface area. Sci Rep, 9(1), 11758. doi:10.1038/s41598-019-48020-8
Fleming, C., Trevors, J. J. W., Air, & Pollution, S. (1989). May Beth Adams, J. Michael Kelly, and Nelson T. Edwards,'Growth of Pinus taeda L. seedlings Varies with Family and Ozone Exposure Level', Water, Air and Soil Pollut. 38, 137. 42(447).
Gebremedhn, K., Kahsay, M. H., Aklilu, M. J. J. o. P., & Pharmacology. (2019). Green synthesis of CuO nanoparticles using leaf extract of catha edulis and its antibacterial activity. 7(7), 327-342.
Gunalan, S., Sivaraj, R., & Rajendran, V. J. P. i. N. S. M. I. (2012). Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. 22(6), 693-700.
Hamelian, M., Hemmati, S., Varmira, K., & Veisi, H. J. J. o. t. T. I. o. C. E. (2018). Green synthesis, antibacterial, antioxidant and cytotoxic effect of gold nanoparticles using Pistacia Atlantica extract. 93, 21-30.
K, V., S, S., P, M., S, M., & S, S. (2021). Ecofriendly green synthesis, characterization and biomedical applications of CuO nanoparticles synthesized using leaf extract of Capsicum frutescens. Journal of Environmental Chemical Engineering, 9(5). doi:10.1016/j.jece.2021.106299
Katwal, R., Kaur, H., Sharma, G., Naushad, M., & Pathania, D. (2015). Electrochemical synthesized copper oxide nanoparticles for enhanced photocatalytic and antimicrobial activity. Journal of Industrial and Engineering Chemistry, 31, 173-184. doi:10.1016/j.jiec.2015.06.021
Kaviyarasu, K., Magdalane, C. M., Jayakumar, D., Samson, Y., Bashir, A., Maaza, M., . . . Kennedy, J. J. J. o. K. S. U.-S. (2020). High performance of pyrochlore like Sm2Ti2O7 heterojunction photocatalyst for efficient degradation of rhodamine-B dye with waste water under visible light irradiation. 32(2), 1516-1522.
Kaviyarasu, K., Mola, G. T., Oseni, S. O., Kanimozhi, K., Magdalane, C. M., Kennedy, J., & Maaza, M. J. J. o. M. S. M. i. E. (2019). ZnO doped single wall carbon nanotube as an active medium for gas sensor and solar absorber. 30(1), 147-158.
Laha, D., Pramanik, A., Laskar, A., Jana, M., Pramanik, P., & Karmakar, P. (2014). Shape-dependent bactericidal activity of copper oxide nanoparticle mediated by DNA and membrane damage. Materials Research Bulletin, 59, 185-191. doi:10.1016/j.materresbull.2014.06.024
LewisOscar, F., MubarakAli, D., Nithya, C., Priyanka, R., Gopinath, V., Alharbi, N. S., & Thajuddin, N. (2015). One pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains of Pseudomonas aeruginosa. Biofouling, 31(4), 379-391. doi:10.1080/08927014.2015.1048686
Mayekar, J., Dhar, V., Radha, S. J. I. J. o. S., & Research, E. (2014). Synthesis of copper oxide nanoparticles using simple chemical route. 5, 928-930.
Mehdizadeh, T., Zamani, A., & Abtahi Froushani, S. M. (2020). Preparation of Cu nanoparticles fixed on cellulosic walnut shell material and investigation of its antibacterial, antioxidant and anticancer effects. Heliyon, 6(3), e03528. doi:10.1016/j.heliyon.2020.e03528
Mittal, A., & Chisti, Y. J. A. andBanerjeeUC2013Biotechnol. 31, 346-356.
Muhammad Hafeez*1, R. A., Jahanzeb Khan2, Bilal Akram2, Muhammad Naeem Ahmad1, Muhammad Usman Hameed3, Sirajul Haq. (2019). Populus ciliata mediated synthesis of copper oxide nanoparticles for potential biological applications. doi:10.1088/2053-1591/ab0601
Nabila, M. I., & Kannabiran, K. (2018). Biosynthesis, characterization and antibacterial activity of copper oxide nanoparticles (CuO NPs) from actinomycetes. Biocatalysis and Agricultural Biotechnology, 15, 56-62. doi:10.1016/j.bcab.2018.05.011
Nethravathi, P., Kumar, M. P., Suresh, D., Lingaraju, K., Rajanaika, H., Nagabhushana, H., & Sharma, S. J. M. S. i. S. P. (2015). Tinospora cordifolia mediated facile green synthesis of cupric oxide nanoparticles and their photocatalytic, antioxidant and antibacterial properties. 33, 81-88.
Nwanya, A. C., Ndipingwi, M. M., Mayedwa, N., Razanamahandry, L., Ikpo, C. O., Waryo, T., . . . Ezema, F. I. J. E. A. (2019). Maize (Zea mays L.) fresh husk mediated biosynthesis of copper oxides: Potentials for pseudo capacitive energy storage. 301, 436-448.
Nwanya, A. C., Razanamahandry, L. C., Bashir, A., Ikpo, C. O., Nwanya, S. C., Botha, S., . . . Maaza, M. J. J. o. h. m. (2019). Industrial textile effluent treatment and antibacterial effectiveness of Zea mays L. Dry husk mediated bio-synthesized copper oxide nanoparticles. 375, 281-289.
Osaili, T. M., Albiss, B. A., Al–Nabulsi, A. A., Alromi, R. F., Olaimat, A., Al‐Holy, M., . . . Holley, R. (2019). Effects of metal oxide nanoparticles with plant extract on viability of foodborne pathogens. Journal of Food Safety, 39(5). doi:10.1111/jfs.12681
Pallela, P. N. V. K., Ummey, S., Ruddaraju, L. K., Kollu, P., Khan, S., & Pammi, S. J. S. A. S. (2019). Antibacterial activity assessment and characterization of green synthesized CuO nano rods using Asparagus racemosus roots extract. 1(5), 1-7.
Pammi5, P. N. V. K. P. S. U. L. K. R. P. K. S. K. S. V. N. (2019). Antibacterial activity assessment and characterization of green synthesized CuO nano rods using Asparagus racemosus roots extract. doi:10.1007/s42452-019-0449-9
Patil, A. S. (2010). . doi:10.5897/JMPR10.061
Phiwdang, K., Suphankij, S., Mekprasart, W., & Pecharapa, W. (2013). Synthesis of CuO Nanoparticles by Precipitation Method Using Different Precursors. Energy Procedia, 34, 740-745. doi:10.1016/j.egypro.2013.06.808
Qamar, H., Rehman, S., Chauhan, D. K., Tiwari, A. K., & Upmanyu, V. J. I. J. o. N. (2020). Green synthesis, characterization and antimicrobial activity of copper oxide nanomaterial derived from Momordica charantia. 15, 2541.
Raffi, M., Mehrwan, S., Bhatti, T. M., Akhter, J. I., Hameed, A., Yawar, W., & ul Hasan, M. M. (2010). Investigations into the antibacterial behavior of copper nanoparticles against Escherichia coli. Annals of Microbiology, 60(1), 75-80. doi:10.1007/s13213-010-0015-6
Rahimi-Nasrabadi, M., Pourmortazavi, S. M., Davoudi-Dehaghani, A. A., Hajimirsadeghi, S. S., & Zahedi, M. M. (2013). Synthesis and characterization of copper oxalate and copper oxide nanoparticles by statistically optimized controlled precipitation and calcination of precursor. CrystEngComm, 15(20). doi:10.1039/c3ce26930b
Rajeshwari Sivaraj a, b., ⇑,1, Pattanathu K.S.M. Rahman a,1, P. Rajiv b,2, S. Narendhran b,2, R. Venckatesh c,3. (2014). Biosynthesis and characterization of Acalypha indica mediated copper oxide nanoparticles and evaluation of its antimicrobial and anticancer activity. doi:10.1016/j.saa.2014.03.027
Rathnakumar, S. S., Noluthando, K., Kulandaiswamy, A. J., Rayappan, J. B. B., Kasinathan, K., Kennedy, J., . . . Chemical, A. B. (2019). Stalling behaviour of chloride ions: a non-enzymatic electrochemical detection of α-Endosulfan using CuO interface. 293, 100-106.
Ren, G., Hu, D., Cheng, E. W., Vargas-Reus, M. A., Reip, P., & Allaker, R. P. (2009). Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents, 33(6), 587-590. doi:10.1016/j.ijantimicag.2008.12.004
S. Suresh, R. I., G. Kalaiyan, S. Thambidurai, P. Kannan, K.M. Prabu, N. Suresh, R. Jothilakshmi, S. Karthick Kumar, M. Kandasamy. (2020). Green Synthesis of Copper Oxide Nanostructures using Cynodon dactylon and Cyperus rotundus Grass Extracts for Antibacterial Applications. doi:10.1016/j.ceramint.2020.02.015
Saif, S., Tahir, A., Asim, T., & Chen, Y. (2016). Plant Mediated Green Synthesis of CuO Nanoparticles: Comparison of Toxicity of Engineered and Plant Mediated CuO Nanoparticles towards Daphnia magna. Nanomaterials (Basel), 6(11). doi:10.3390/nano6110205
Sankar, R., Karthik, A., Prabu, A., Karthik, S., Shivashangari, K. S., Ravikumar, V. J. C., & Biointerfaces, S. B. (2013). Origanum vulgare mediated biosynthesis of silver nanoparticles for its antibacterial and anticancer activity. 108, 80-84.
Sankar, R., Maheswari, R., Karthik, S., Shivashangari, K. S., & Ravikumar, V. (2014). Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles. Mater Sci Eng C Mater Biol Appl, 44, 234-239. doi:10.1016/j.msec.2014.08.030
Sankar, R., Prasath, B. B., Nandakumar, R., Santhanam, P., Shivashangari, K. S., & Ravikumar, V. (2014). Growth inhibition of bloom forming cyanobacterium Microcystis aeruginosa by green route fabricated copper oxide nanoparticles. Environ Sci Pollut Res Int, 21(24), 14232-14240. doi:10.1007/s11356-014-3362-1
Sarkar, J., Chakraborty, N., Chatterjee, A., Bhattacharjee, A., Dasgupta, D., & Acharya, K. (2020). Green Synthesized Copper Oxide Nanoparticles Ameliorate Defence and Antioxidant Enzymes in Lens culinaris. Nanomaterials (Basel), 10(2). doi:10.3390/nano10020312
Sathiyavimal, S., Vasantharaj, S., Bharathi, D., Saravanan, M., Manikandan, E., Kumar, S. S., & Pugazhendhi, A. (2018). Biogenesis of copper oxide nanoparticles (CuONPs) using Sida acuta and their incorporation over cotton fabrics to prevent the pathogenicity of Gram negative and Gram positive bacteria. J Photochem Photobiol B, 188, 126-134. doi:10.1016/j.jphotobiol.2018.09.014
Sau, T. K., & Rogach, A. L. J. A. M. (2010). Nonspherical noble metal nanoparticles: colloid‐chemical synthesis and morphology control. 22(16), 1781-1804.
Seerangaraj Vasantharaj, S. S., Elayaperumal, Manikandan, PII:, P., Reference:, D., date:, T. a. i. R., date:, R. d. A., . . . Arivalaga. (2018). Synthesis of ecofriendly copper oxide nanoparticles for fabrication over textile fabrics: Characterization of antibacterial activity and dye degradation potential. doi:10.1016/j.jphotobiol.2018.12.026
1016/ACCEPTED
Sepasgozar, S. M. E., Mohseni, S., Feizyzadeh, B., & Morsali, A. J. B. o. M. S. (2021). Green synthesis of zinc oxide and copper oxide nanoparticles using Achillea Nobilis extract and evaluating their antioxidant and antibacterial properties. 44(2), 1-13.
Shaheen, T. I., Fouda, A., Salem, S. S. J. I., & Research, E. C. (2021). Integration of cotton fabrics with biosynthesized CuO nanoparticles for bactericidal activity in the terms of their cytotoxicity assessment. 60(4), 1553-1563.
Shahsavani, E., Feizi, N., & DEHNO, K. A. (2016). Copper oxide nanoparticles prepared by solid state thermal decomposition: synthesis and characterization.
Shameli, K., Bin Ahmad, M., Jaffar Al-Mulla, E. A., Ibrahim, N. A., Shabanzadeh, P., Rustaiyan, A., . . . Zidan, M. (2012). Green biosynthesis of silver nanoparticles using Callicarpa maingayi stem bark extraction. Molecules, 17(7), 8506-8517. doi:10.3390/molecules17078506
Silva, N., Ramirez, S., Diaz, I., Garcia, A., & Hassan, N. (2019). Easy, Quick, and Reproducible Sonochemical Synthesis of CuO Nanoparticles. Materials (Basel), 12(5). doi:10.3390/ma12050804
Sorbiun1, M., & , E. S. M., Ali Ramazani1,2 *, Asemeh Mashhadi Malekzadeh1. (2018). . doi:10.22036/ncr.2018.01.001
Sreeju, N., Rufus, A., & Philip, D. (2017). Studies on catalytic degradation of organic pollutants and anti-bacterial property using biosynthesized CuO nanostructures. Journal of Molecular Liquids, 242, 690-700. doi:10.1016/j.molliq.2017.07.077
Ssekatawa, K., Byarugaba, D. K., Angwe, M. K., Wampande, E. M., Ejobi, F., Nxumalo, E., . . . biotechnology. (2022). Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances. 10.
Ssekatawa, K., Byarugaba, D. K., Kato, C. D., Ejobi, F., Tweyongyere, R., Lubwama, M., . . . Wampande, E. M. J. J. o. N. R. (2020). Nanotechnological solutions for controlling transmission and emergence of antimicrobial-resistant bacteria, future prospects, and challenges: a systematic review. 22(5), 1-30.
Tshireletso, P., Ateba, C. N., & Fayemi, O. E. (2021). Spectroscopic and Antibacterial Properties of CuONPs from Orange, Lemon and Tangerine Peel Extracts: Potential for Combating Bacterial Resistance. Molecules, 26(3). doi:10.3390/molecules26030586
Wang, B., Wu, X.-L., Shu, C.-Y., Guo, Y.-G., & Wang, C.-R. J. J. o. M. C. (2010). Synthesis of CuO/graphene nanocomposite as a high-performance anode material for lithium-ion batteries. 20(47), 10661-10664.
Xie, H., Wang, J.-R., Yau, L.-F., Liu, Y., Liu, L., Han, Q.-B., . . . Jiang, Z.-H. J. M. (2014). Quantitative analysis of the flavonoid glycosides and terpene trilactones in the extract of Ginkgo biloba and evaluation of their inhibitory activity towards fibril formation of β-amyloid peptide. 19(4), 4466-4478.
Yang, H., Liu, Q., Li, F., Jin, C., & Yu, R. J. A. p. l. (2006). Symmetry of unoccupied electronic states in the high-T c superconductor Sr 2 CuO 2+ δ Cl 2− y studied by electron energy-loss spectroscopy. 88(8), 082502.
Nyandra, M., Kartiko, B.H., Susanto, P.C., Supriyati, A., Suryasa, W. (2018). Education and training improve quality of life and decrease depression score in elderly population. Eurasian Journal of Analytical Chemistry, 13(2), 371-377.
Widyaningrum , I. ., Wibisono, N. ., & Kusumawati, A. H. . (2020). Effect of extraction method on antimicrobial activity against staphylococcus aureus of tapak liman (elephantopus scaber l.) leaves. International Journal of Health & Medical Sciences, 3(1), 105-110. https://doi.org/10.31295/ijhms.v3n1.181
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