Association between T-182C polymorphism of the norepinephrine transporter SLC6A2(NET) gene and major depressive disorder in Iraqi patients
Keywords:
norepinephrine neurotransmitter, norepinephrine receptors, ARMS-PCR, major depression disorder, SLC6A2Abstract
Major sympathetic nervous system neurotransmitter that is responsible for consciousness, attention, cognitive ability, as well as the function of the majority of body systems, glands, and the immune system, is called Norepinephrine (NE). Norepinephrine transporter (NET) and norepinephrine binding sites are decreased in the brains of MDD patients, showing that dysregulated synaptic NE plays a role in the pathophysiology of MDD. Indeed, different NET polymorphism combinations may be linked to different MDD sub-phenotypes. The aim of the current work is to investigate or detect if there is a relation between T-182C gene polymorphism of SLC6A2 gene with major depressive disorder (MDD) in Iraqi patients.Materials and Methods: Blood specimens were collected from 70 individuals with MDD and 20 healthy individuals as a control group (both patients and control ages ranged between 18-65 years). DNA extracted from white blood cells (WBC), and the T-182C gene was detected and amplified by the PCR-ARMS technique. Results: The distribution of TT, TC, and CC genotype ratio of MDD group was 22.9%, 27.1%, and 50.0%, respectively. While the control group show genotype distribution as 35.0% for TT, 55.0% for TC and 10.0% for CC ratio.
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Ménard, C., G.E. Hodes, and S.J. Russo, Pathogenesis of depression: Insights from human and rodent studies. Neuroscience, 2016. 321: p. 138-162.
Delva, N.C. and G.D. Stanwood, Dysregulation of brain dopamine systems in major depressive disorder. Experimental Biology and Medicine, 2021. 246 (9): p. 1084-1093.
Shadrina, M., E.A. Bondarenko, and P.A. Slominsky, Genetics factors in major depression disease. Frontiers in psychiatry, 2018. 9: p. 334.
Zaki, N.F., et al., Chronobiological theories of mood disorder. European archives of psychiatry and clinical neuroscience, 2018. 268(2): p. 107-118.
O’Donnell, J., et al., Norepinephrine: a neuromodulator that boosts the function of multiple cell types to optimize CNS performance. Neurochemical research, 2012. 37(11): p. 2496-2512.
Gannon, M., et al., Noradrenergic dysfunction in Alzheimer's disease. Frontiers in neuroscience, 2015: p. 220.
Terbeck, S., et al., Noradrenaline effects on social behaviour, intergroup relations, and moral decisions. Neuroscience & Biobehavioral Reviews, 2016. 66: p. 54-60.
Ramos, B. and A. Arnsten, Adrenerg farmakologi og kognisjon: fokus på den prefrontale cortex. Pharmacol. ther, 2007. 113: p. 523-536.
Arnsten, A.F., M.J. Wang, and C.D. Paspalas, Neuromodulation of thought: flexibilities and vulnerabilities in prefrontal cortical network synapses. Neuron, 2012. 76(1): p. 223-239.
Jin, X., et al., Identification of a group of GABAergic neurons in the dorsomedial area of the locus coeruleus. PloS one, 2016. 11(1): p. e0146470.
Hahn, M.K. and R.D. Blakely, The functional impact of SLC6 transporter genetic variation. Annu. Rev. Pharmacol. Toxicol., 2007. 47: p. 401-441.
Ueda, I., et al., Relationship between G1287A of the NET gene polymorphisms and brain volume in major depressive disorder: a voxel-based MRI study. PloS one, 2016. 11(3): p. e0150712.
Haenisch, B., et al., Knockout of the norepinephrine transporter and pharmacologically diverse antidepressants prevent behavioral and brain neurotrophin alterations in two chronic stress models of depression. Journal of neurochemistry, 2009. 111(2): p. 403-416.
Lin, Z. and B. Madras, Human genetics and pharmacology of neurotransmitter transporters. Neurotransmitter transporters, 2006: p. 327-371.
Ryu, S.-H., et al., Association between norepinephrine transporter gene polymorphism and major depression. Neuropsychobiology, 2004. 49(4): p. 174-177.
Cao, S.-X., et al., Association between T-182C, G1287A polymorphism in NET gene and suicidality in major depressive disorder in Chinese patients. International Journal of Psychiatry in Clinical Practice, 2018. 22(4): p. 304-309.
Inoue, K., et al., Positive association between T-182C polymorphism in the norepinephrine transporter gene and susceptibility to major depressive disorder in a Japanese population. Neuropsychobiology, 2004. 50(4): p. 301-304.
Kim, C.-H., et al., A previously undescribed intron and extensive 5′ upstream sequence, but not Phox2a-mediated transactivation, are necessary for high level cell type-specific expression of the human norepinephrine transporter gene. Journal of Biological Chemistry, 1999. 274(10): p. 6507-6518.
Xu, Y., et al., The norepinephrine transporter gene modulates the relationship between urban/rural residency and major depressive disorder in a Chinese population. Psychiatry research, 2009. 168(3): p. 213-217.
Min, W., et al., Monoamine transporter gene polymorphisms affect susceptibility to depression and predict antidepressant response. Psychopharmacology, 2009. 205(3): p. 409-417.
Zill, P., et al., Identification of a naturally occurring polymorphism in the promoter region of the norepinephrine transporter and analysis in major depression. Neuropsychopharmacology, 2002. 26(4): p. 489-493.
Chang, C.-C., et al., Lack of association between the norepinephrine transporter gene and major depression in a Han Chinese population. Journal of Psychiatry and Neuroscience, 2007. 32(2): p. 121-128.
Zhao, X., et al., Association between major depressive disorder and the norepinephrine transporter polymorphisms T-182C and G1287A: a meta-analysis. Journal of affective disorders, 2013. 150(1): p. 23-28.
Kautto, M., et al., Serotonin transporter (5-HTTLPR) and norepinephrine transporter (NET) gene polymorphisms: susceptibility and treatment response of electroconvulsive therapy in treatment resistant depression. Neuroscience letters, 2015. 590: p. 116-120.
Cottingham, C. and Q. Wang, α2 adrenergic receptor dysregulation in depressive disorders: Implications for the neurobiology of depression and antidepressant therapy. Neuroscience & Biobehavioral Reviews, 2012. 36(10): p. 2214-2225.
Andrade, C. and S. Sudha, Electroconvulsive therapy and the alpha-2 noradrenergic receptor: implications of treatment schedule effects. The journal of ECT, 2000. 16(3): p. 268-278.
Kohli, U., et al., Genetic variation in the presynaptic norepinephrine transporter is associated with blood pressure responses to exercise in healthy humans. Pharmacogenetics and genomics, 2011. 21(4): p. 171.
Miret, M., et al., Depressive disorders and suicide: epidemiology, risk factors, and burden. Neuroscience & Biobehavioral Reviews, 2013. 37(10): p. 2372-2374.
Lancini, E., et al., CSF and PET biomarkers for noradrenergic dysfunction in neurodegenerative disease: A systematic review and meta‐analysis. Alzheimer's & Dementia, 2021. 17: p. e055942.
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Get vaccinated when it is your turn and follow the local guidelines. International Journal of Health Sciences, 5(3), x-xv. https://doi.org/10.53730/ijhs.v5n3.2938
Suryasa, I. W., Rodríguez-Gámez, M., & Koldoris, T. (2021). Health and treatment of diabetes mellitus. International Journal of Health Sciences, 5(1), i-v. https://doi.org/10.53730/ijhs.v5n1.2864
Batara, R., Ardani, I. G. A. I., Wardani, I. A. K., Windiani, I. G. A. T., Adnyana, I. G. A. N. S., & Setiawati, Y. (2022). Psychogenic dysphagia in children, and the success of family-based treatment: Case report. International Journal of Health & Medical Sciences, 5(1), 163-168. https://doi.org/10.21744/ijhms.v5n1.1894
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