Impact of prodigiosin on staphylococcus aureus isolated from acne vulgaris
Keywords:
staphylococcus aureus, prodigiosin, IcaA gene, lipase, biofilmAbstract
Acne is a skin disorder characterized by clogged hair follicles and chronic inflammation of the pilosebaceous follicles. A total of one hundred and five acne swabs were collected from patients with acne vulgaris infection at Baghdad's hospitals. For primary identification, 54 (45%) samples had a positive S. aureus culture on Mannitol salt agar (MSA) for 24 hours at 37°C. In addition, four pre-isolated also identified S. marcescens (pigment producers) which were identified by vitek-2 technique were taken for prodigiosin extraction, mineral salt broth with peptone (0.5 percent) was utilized, followed by pigment extraction, partial purification, also pigment measurement by spectrophotometer. Furthermore, using a microtiter plate assay, the bacterial ability to form biofilms was assessed for 20 S. aureus isolates (multidrug resistant isolates). The results revealed that only 8 strong isolates were biofilm producers, while 5 in addition 7 isolates were moderate also weak biofilm producers. The minimal inhibitory concentration of tetracycline also prodigiosin generated by isolated S. marcescens were determined using the broth microdilution technique for S. aureus isolates (S7 also S8). From overnight cultures of S. aureus (S7 also S8), DNA was effectively extracted.
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