Qualitative, quantitative phytochemical analysis and in-vitro anti-mycobacterial evaluation of allspice
Keywords:
Pimenta dioica, MABA, Alkaloids, flavonoids, phenolsAbstract
Phytochemical study of plant products has gained popularity in both plant biochemistry and organic chemistry in recent years. One of the current issues in phytochemistry is carrying out all of the above operations with little amounts of material. Pimenta dioica Linn, often called as "All-spice," contains a number of secondary metabolites with medicinal potential. This study examines the key phytochemicals found in the leaves of the medicinally important plant Pimenta dioica on a qualitative and quantitative level. The goal of this study is to evaluate phytochemicals by analysing diverse extracts quantitatively and qualitatively. Quantification and phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, proteins, amino acids, saponins, tannins, terpenoids, and glycosides. In addition, the study found that an ethanolic extract of leaves extract was more effective against mycobacterium tuberculin. A microplate-based Alamar blue assay has been applied to search for novel antimicrobials to treat tuberculosis from phytochemicals in Pimenta dioica.
Downloads
References
Ajiboye, B.O., Ibukun, E.O., Edobor, G., Ojo, A.O., Onikanni S. (2013). Qualitative and quantitative analysis of phytochemicals in Senecio biafrae leaf. Int. J. Inv. Pharm. Sci., 1(5); 428-432.
Borris, R.P. (1996). Natural products research: perspectives from a major pharmaceutical company. J. Nat Prod Res., 51, 29-35.
Brooks, J.T., Kaplan, J.E., Holmes, K.K., Benson, C., Pau, A. & Masur, H. (2009). HIV-Associated Opportunistic Infections-Going, Going, but Not Gone: The Continued Need for Prevention and Treatment Guidelines. Clin Infect Dis, 48(6): 09–11.
Chukwuma, S.E. & Chigozie, M.E. (2016). Qualitative and quantitative determination of phytochemical contents of indigenous Nigerian softwoods. New J of Sci. 1-9.
Collins, L.A, Franzblau, S.G. (1997). Microplate Alamar Blue assay versus BACTEC 460 for high-throughput screening of compounds again Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009.
Dhrubo, J.S. (2016). Oregano: the mountain of joy on taste buds, World J of Pharma Science, 4, 226-234.
Durai, M.V., Balamuniappan, G., Anandalakshmi, R., Geetha, S. & Senthil Kumar, N. (2016). Qualitative and quantitative analysis of phytochemicals in crude extract of big – Leaf mahogany (Swietenia macrophylla King.). Inter. J. of Herbal Medicine, 4(6): 88-91.
Dye, C., Scheele, S., Dolin, P., Pathania, V.& Raviglione, M.C. (1999). WHO Global Surveillance and Monitoring Project. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. JAMA, 282:677-686.
Dye, C. and Williams, B.G. (2010) The Population Dynamics and Control of Tuberculosis. Science, 328, 856-861.
Edem, B.E., Khan, M.E., Ibok, N.U. & Dimlong, L.I. (2016). Qualitative & quantitative phytochemical screening and proximate composition of Bombax buonopozense (Red Silk Cotton Tree) Stem-Back. Journal of Adv. in Nat. Sci., 3(3); 288 -292.
Ekwueme, F.N., Nwodo, O.F. C., Joshua, P.E., Nkwocha, C.& Eluka, P.E. (2015). Qualitative and quantitative phytochemical screening of the aqueous leaf extract of Senna mimosoides : Its effect in in vivo leukocyte mobilization induced by inflammatory stimulus. Int. J. Curr. Microbiol. App. Sci., 4(5): 1176-1188.
Franzblau, S.G. & Witzig, R.S. (1998). Low technology MIC determination with clinical Mycobacterium tuberculosis isolates by using Microplate Almar blue assay. J Clin.Microbiol. 36:362-366.
Gokhale, S.B., Purohit, A.P. & Kokate, C.K. (2020). Pharmacognosy, Pune, Maharashtra: Nirali Prakashan.
Grange, J.M., Schaaf, S.& Zumla, A.L., (2009). Tuberculosis, Saunders,44–59.
Gul, R., Syed, U., Syed, F., Samiullah, S. & Jahan, N. (2017). Preliminary phytochemical screening, quantitative analysis of alkaloids and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to balochistan. The Scientific World Journal, 1-7.
Habila, J.D., Bello, I.A., Dzikwe, A.A., Ladan, Z. & Sabiu, M. (2011). Comparative evaluation of phytochemicals, antioxidant and antimicrobial activity of four medicinal plants native to northern nigeria. Australian J. Basic and App. Sci. 5(5), 537.
Hagerman, A., Muller,I. & Makkar, H. (2000). Quantification of tannins in tree foliage. A laboratory manual, Vienna, 4-7.
Harborne, J.B. (1998). Phytochemical Methods. London: Springer International.
Khandelwal, K.R. (2005) Practical Pharmacognosy Technique and Experiments. Pune, Maharashtra: Nirali Prakashan.
Lorence, D.H., Flynn, T.W. & Wagner, W.L. (1995). Contributions to the Flora of Hawaiian Bishop Museum Occasional Papers, Honolulu, Hawaii: Bishop Museum Press, 41, 19–58.
Mandewale, M.C., Thorat, B.R. & Yamgar, R.S. (2015). Synthesis and anti-mycobacterium study of some fluorine containing Schiff bases of quinoline and their metal complexes. Der Pharma Chemica, 7(5):207-215.
Maria, C.S. Lourenco, Marcus, V.N. Desouza & Alessandra, C. Pinheiro. (2007). Evaluation of Anti-Tubercular activity of nicotinic and isoniazid analogues. ARKIVOC., 181-191.
Mir, M.A., Sawhney, S.S. & Jassal, M.M.S. (2013). Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale, Wudpecker. J. Pharm. Pharmocol.,2(1), 01-05.
Mukherjee, P.K. (2002). Quality Control of Herbal Drugs. New Delhi: Business Horizon Pharmaceutical Publishers.
Prabhavathi, R.M., Prasad, M.P.& Jayaramu, M. (2016). Studies on Qualitative and quantitative phytochemical analysis of Cissus quadrangularis. Adv. in App. Sci. Res., 7(4), 11-17.
Rao, N.B. & Kumara, O.S. (2015). Phyto chemical analysis of all spices Pimenta dioica leaf extract, World J of Pharmacy and Pharma Sci. 4, 1400-1404.
Roghini, R. & Vijayalakshmi, K. (2018). Phytochemical screening, quantitative analysis of flavonoids and minerals in ethanolic extract of Citrus paradisi. Int J of Pharma Sci and Res.,9(11): 4859-4864.
Schecter, G.F., Scott, C., True, L., Raftery, A., Flood, J. & Mase, S. (2010). Linezolid in the Treatment of Multidrug-Resistant Tuberculosis. Clinical Infectious Diseases, 50(1): 49–55.
Rinartha, K., & Suryasa, W. (2017). Comparative study for better result on query suggestion of article searching with MySQL pattern matching and Jaccard similarity. In 2017 5th International Conference on Cyber and IT Service Management (CITSM) (pp. 1-4). IEEE.
Rinartha, K., Suryasa, W., & Kartika, L. G. S. (2018). Comparative Analysis of String Similarity on Dynamic Query Suggestions. In 2018 Electrical Power, Electronics, Communications, Controls and Informatics Seminar (EECCIS) (pp. 399-404). IEEE.
Kurniawan, A., Turchan, A., Utomo, B., Parenrengi, M. A., & Fauziah, D. (2022). The change of BDNF expression in traumatic brain injury after Kaempferia galanga L. administration: An experimental study. International Journal of Health & Medical Sciences, 5(1), 101-113. https://doi.org/10.21744/ijhms.v5n1.1847
Published
How to Cite
Issue
Section
Copyright (c) 2022 International journal of health sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles published in the International Journal of Health Sciences (IJHS) are available under Creative Commons Attribution Non-Commercial No Derivatives Licence (CC BY-NC-ND 4.0). Authors retain copyright in their work and grant IJHS right of first publication under CC BY-NC-ND 4.0. Users have the right to read, download, copy, distribute, print, search, or link to the full texts of articles in this journal, and to use them for any other lawful purpose.
Articles published in IJHS can be copied, communicated and shared in their published form for non-commercial purposes provided full attribution is given to the author and the journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
This copyright notice applies to articles published in IJHS volumes 4 onwards. Please read about the copyright notices for previous volumes under Journal History.








